Automated liquid chromatographic analysis of the anti-tumorigenic drugs etoposide (VP 16-213) and teniposide (VM 26)

Werkhoven-Goewie, C.E.; Brinkman, U.A.Th.; Frei, R.W.; Ruiter, C. De; Vries, J. de

doi:10.1016/s0378-4347(00)85101-4

Cited by 43 publications

(5 citation statements)

References 9 publications

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“…In order to understand the distribution of etoposide into tumor it is necessary to develop a sensitive method for quantifying the drug in different biological matrices. Several methods have been used for the determination of etoposide in plasma using high-performance liquid chromatography (HPLC) with UV detection (Holthius et al, 1981;Shirazi et al, 2001;Kato et al, 2003;Dhanaraju et al, 2013;Zhang et al, 2010), fluorescence detection (Strife et al, 1981;Werkhoven-Goewie et al, 1983;Manouilov et al, 1998) and electrochemical detection (Duncan et al, 1986;Cai et al, 1999;Pérez-Urizar et al, 1996). Modern chromatographic methods, such as high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-qTOF-MS/MS) have also been published for etoposide quantification in biological matrices (Liu et al, 2011;Sachin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

HPLC‐UV method for quantifying etoposide in plasma and tumor interstitial fluid by microdialysis: application to pharmacokinetic studies

Pigatto

Mossmann

Costa

2014

Biomedical Chromatography

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A simple and sensitive bioanalytical method was developed and validated for determination of etoposide in plasma and microdialysis samples of Walker-256 tumor-bearing rats. A microdialysis probe was implanted in the center of a subcutaneous tumor and Ringer's solution was used as perfusion medium. Chromatographic separation was conducted on a Shimadzu CLC-C8 column using a mobile phase consisting of water-acetonitrile (70:30; v/v) adjusted to pH 4.0 ± 0.1 with formic acid at a gradient flow rate of 1.0-0.6 mL/min, an injection volume of 30 μL and UV detection at 210 nm. Microdialysate samples were analyzed without processing and plasma samples (100 μL) were spiked with phenytoin as internal standard (IS) (1 µg/mL) followed by extraction with tert-butyl methyl ether. The organic layer was evaporated and reconstituted with 100 μL of mobile phase before injection. The methods for plasma and microdialysate were linear in the ranges of 25-10,000 ng/mL and of 10-1500 ng/mL, respectively. All the validation parameters such as intra- and inter-day precision and accuracy and stability were within the limits established by international guidelines. The present method was successfully applied in the investigation of etoposide pharmacokinetics in rat plasma and microdialysate tumor samples following a single 15 mg/kg intravenous dose.

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Section: Introductionmentioning

confidence: 99%

HPLC‐UV method for quantifying etoposide in plasma and tumor interstitial fluid by microdialysis: application to pharmacokinetic studies

Pigatto

Mossmann

Costa

2014

Biomedical Chromatography

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“…Nagai et al (1998) reported the determination of teniposide in human plasma using UV detection, but the sensitivity was low (1 μg/mL; Nagail et al, 1998). Reported analytical methods are time-consuming or require a long analysis time (Canal et al, 1986;Werkhoven-Goewie et al, 1983;Strife, 1981), and do not meet the requirements for the high-throughput determination of biological samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of teniposide in rat plasma by ultra performance liquid chromatography electrospray ionization tandem mass spectrometry after intravenous administration

Wang

Zhang

Guan

et al. 2009

Biomedical Chromatography

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A novel, specific and rapid ultra performance liquid chromatography electrospray ionization tandem mass spectrometry method has been developed and validated for determination of teniposide in rat plasma. A one-step liquid-liquid extraction method was used and the separation was carried out on an Acquity UPLC(TM) BEH C(18) column with gradient elution using a mobile phase composed of acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.20 mL/min. A triple quadrupole tandem mass spectrometer in multiple-reaction monitoring mode via an electrospray ionization interface was used for the detection of teniposide. The detection was complete within 3.0 min. A linear calibration curve was obtained over the concentration range 10-10,000 ng/mL for teniposide, with a lower limit of quantification of 10 ng/mL. The intra-day precision and inter-day precision (relative standard deviation) were less than 10.23 and 13.09%, respectively. The developed method was applied for the first time to the pharmacokinetic study of teniposide in rats following a single intravenous administration of 4.5 mg/kg teniposide.

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“…For the determination of etoposide and teniposide, several HPLC systems have been reported, mostly using UV [4-8] and fluorescence detection [9,10]. For the determination of the hydroxy acid metabolite, a method involving fluorescence detection was described by Strife et al.…”

Section: Introductionmentioning

confidence: 99%

Computerized cyclic voltammetric detectionafter HPLC of the antineoplastic agentsetoposide, teniposide, adriamycin and itsmetabolite adriamycinol in urine samples

Ploegmakers

Moritz

Toll

et al. 1989

Journal of Analytical Methods in Chemistry

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A computerized electrochemical detection system for application after HPLC, provided with a cyclic voltammetric oxidative and reductive module, is described for the on-line qualitative determination of electroactive antineoplastic agents and metabolites in urine samples, collected from cancer patients, following intravenous administration. The application of two cyclic voltammetric detection modes provides an insight into both oxidative and reductive electrode reactions of compounds, passing the detector and the occurrence of (it)reversible chemical and electrochemical processes at the electrode surface. In this way, redox properties of drugs and metabolites characteristic of their molecular structure, can be established, which may provide information related to their (enzymatic) bioactivation. In the cyclic voltammetric mode, the system permits automatic detection of a compound in the cell, recording, storage and plotting of voltammograms and calculation of the retention times, the half-wave potentials and the peak potentials of each scan of all individual compounds. For routine use, storage of 68 voltammograms on-line is sufficient for the analysis of biological samples in clinical-pharmacological research. Special attention has been paid to automatic, multi-reference-point component detection. Based on their concentrations in urine, the oxidative cyclic voltammetric mode, using a glassy carbon electrode, permits the determination of etoposide and teniposide, whereas the reductive cyclic voltammetric mode, with a static mercury drop electrode, permits the determination of adriamycin and its metabolite adriamycinol.

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Automated liquid chromatographic analysis of the anti-tumorigenic drugs etoposide (VP 16-213) and teniposide (VM 26)

Cited by 43 publications

References 9 publications

HPLC‐UV method for quantifying etoposide in plasma and tumor interstitial fluid by microdialysis: application to pharmacokinetic studies

HPLC‐UV method for quantifying etoposide in plasma and tumor interstitial fluid by microdialysis: application to pharmacokinetic studies

Determination of teniposide in rat plasma by ultra performance liquid chromatography electrospray ionization tandem mass spectrometry after intravenous administration

Computerized cyclic voltammetric detectionafter HPLC of the antineoplastic agentsetoposide, teniposide, adriamycin and itsmetabolite adriamycinol in urine samples

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