Automated laser-assisted synthesis of microarrays for infectious disease research

Paris, Grigori; Heidepriem, Jasmin; Tsouka, Alexandra; Mende, Marco; Eickelmann, Stephan; Loeffler, Felix F.

doi:10.1117/12.2516781

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…Thus, to have a more reliable prediction, a minimization of the plastic deformation is desirable. In a previous work [42], material deposition with laser powers below 60 mW have been observed, although we cannot detect surface expansions for those powers. Thus, for laser powers below the 60 mW threshold, it is assumed that only a minimal surface expansion occurs and a negligible amount of plastic deformation takes place, resulting in an almost completely reversible process.…”

Section: Quantitative Analysis Of the Donor Surface Expansioncontrasting

confidence: 63%

“…A complete description of the used lasing setup is available in [42]. In brief, the lasing system contains a 405 nm wavelength laser, which has a Gaussian beam profile of up to 300 mW power (iBeam smart 405-S, TOPTICA Photonics AG, Germany) that is coupled to a laser scanning system (intelliSCAN III 10, SCANLAB, Germany), equipped with an F-Theta-lens (JENar 170-355-140, JENOPTIK Optical Systems GmbH, Germany).…”

Section: Lasing Setupmentioning

confidence: 99%

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Laser-induced forward transfer of soft material nanolayers with millisecond pulses shows contact-based material deposition

Paris

Klinkusch

Heidepriem

et al. 2020

Applied Surface Science

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Section: Quantitative Analysis Of the Donor Surface Expansioncontrasting

confidence: 63%

Section: Lasing Setupmentioning

confidence: 99%

Laser-induced forward transfer of soft material nanolayers with millisecond pulses shows contact-based material deposition

Paris

Klinkusch

Heidepriem

et al. 2020

Applied Surface Science

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“…To our knowledge, this work is the first, showing the synthesis of glycopeptides with defined valencies and spacing in situ on different commercially available microarrays to investigate the effect of substrate functionalization. Our technology relies on readily available compounds (Eickelmann et al, 2019) and can be fully automated (Paris et al, 2019). This enables us to screen a diverse collection of glycopeptides with their corresponding lectins.…”

Section: Discussionmentioning

confidence: 99%

Probing Multivalent Carbohydrate-Protein Interactions With On-Chip Synthesized Glycopeptides Using Different Functionalized Surfaces

et al. 2021

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Multivalent ligand–protein interactions are a commonly employed approach by nature in many biological processes. Single glycan–protein interactions are often weak, but their affinity and specificity can be drastically enhanced by engaging multiple binding sites. Microarray technology allows for quick, parallel screening of such interactions. Yet, current glycan microarray methodologies usually neglect defined multivalent presentation. Our laser-based array technology allows for a flexible, cost-efficient, and rapid in situ chemical synthesis of peptide scaffolds directly on functionalized glass slides. Using copper(I)-catalyzed azide–alkyne cycloaddition, different monomer sugar azides were attached to the scaffolds, resulting in spatially defined multivalent glycopeptides on the solid support. Studying their interaction with several different lectins showed that not only the spatially defined sugar presentation, but also the surface functionalization and wettability, as well as accessibility and flexibility, play an essential role in such interactions. Therefore, different commercially available functionalized glass slides were equipped with a polyethylene glycol (PEG) linker to demonstrate its effect on glycan–lectin interactions. Moreover, different monomer sugar azides with and without an additional PEG-spacer were attached to the peptide scaffold to increase flexibility and thereby improve binding affinity. A variety of fluorescently labeled lectins were probed, indicating that different lectin–glycan pairs require different surface functionalization and spacers for enhanced binding. This approach allows for rapid screening and evaluation of spacing-, density-, ligand and surface-dependent parameters, to find optimal lectin binders.

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“…The lasing setup is part of an automated synthesizer, which was previously reported [33]. It contains a 405 nm wavelength diode laser (iBeam smart 405-S, TOPTICA Photonics AG, Munich, Germany) that has a Gaussian beam profile and a maximum power of 300 mW, which is only used in the continuous wave regime (>10 −6 s).…”

Section: Lasing Setupmentioning

confidence: 99%

Development and Experimental Assessment of a Model for the Material Deposition by Laser-Induced Forward Transfer

Paris

Bierbaum

Paris³

et al. 2022

Applied Sciences

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The potential to deposit minute amounts of material from a donor to an acceptor substrate at precise locations makes laser-induced forward transfer (LIFT) a frequently used tool within different research fields, such as materials science and biotechnology. While many different types of LIFT exist, each specialized LIFT application is based on a different underlying transfer mechanism, which affects the to-be-transferred materials in different ways. Thus, a characterization of these mechanisms is necessary to understand their limitations. The most common investigative methods are high-speed imaging and numerical modeling. However, neither of these can, to date, quantify the material deposition. Here, analytical solutions are derived for the contact-based material deposition by LIFT, which are based on a previously observed equilibrium state. Moreover, an analytical solution for the previously unrecognized ejection-based material deposition is proposed, which is detectable by introducing a distance between the donor and acceptor substrates. This secondary mechanism is particularly relevant in large scale production, since each deposition from a donor substrate potentially induces a local distance between the donor and acceptor substrates.

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Automated laser-assisted synthesis of microarrays for infectious disease research

Cited by 4 publications

References 15 publications

Laser-induced forward transfer of soft material nanolayers with millisecond pulses shows contact-based material deposition

Laser-induced forward transfer of soft material nanolayers with millisecond pulses shows contact-based material deposition

Probing Multivalent Carbohydrate-Protein Interactions With On-Chip Synthesized Glycopeptides Using Different Functionalized Surfaces

Development and Experimental Assessment of a Model for the Material Deposition by Laser-Induced Forward Transfer

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