Automated kinetic determination of angiotensin-converting enzyme in serum.

Harjanne, Aira

doi:10.1093/clinchem/30.6.901

Cited by 26 publications

(4 citation statements)

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“…ACE extract (10 μl) was added to the mixture and the absorbance at 340 nm was measured using an ELISA reader Expert 96 (ASYS Hi-tech, Eugendorf, Austria) over a period of 25 min at 37°C. For determination of the IC 50 value for ACE-inhibitory activity of each peptide fraction, the activity of ACE in units/l was calculated according to the following formula (Harjanne 1984):where V t is the final assay volume; V s is the volume of ACE present in the assay; d is the light path (cm); Δε is the absorbance change at 340 nm obtained by the complete hydrolysis of 1 m m of FAPGG to FAP and GG and ΔA/min is the decrease in absorbance at 340 nm per minute. The IC 50 value was determined from a plot of percentage of ACE inhibitory activity against the peptide concentration.…”

Section: Methodsmentioning

confidence: 99%

Section: Determination Of the Ace-inhibitory Activitymentioning

confidence: 99%

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Biological activity of camel milk casein following enzymatic digestion

Salami

Moosavi‐Movahedi

Moosavi-Movahedi

et al. 2011

Journal of Dairy Research

134

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The aim of this study was to investigate the effects of enzymatic hydrolysis with digestive enzymes of camel whole casein and beta-casein (β-CN) on their antioxidant and Angiotensin Converting Enzyme (ACE)-inhibitory properties. Peptides in each hydrolysate were fractionated with ultra-filtration membranes. The antioxidant activity was determined using a Trolox equivalent antioxidant capacity (TEAC) scale. After enzymatic hydrolysis, both antioxidant and ACE-inhibitory activities of camel whole casein and camel β-CN were enhanced. Camel whole casein and β-CN showed significant ACE-inhibitory activities after hydrolysis with pepsin alone and after pepsinolysis followed by trypsinolysis and chymotrypsinolysis. Camel β-CN showed high antioxidant activity after hydrolysis with chymotrypsin. The results of this study suggest that when camel milk is consumed and digested, the produced peptides start to act as natural antioxidants and ACE-inhibitors.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of the Ace-inhibitory Activitymentioning

confidence: 99%

Biological activity of camel milk casein following enzymatic digestion

Salami

Moosavi‐Movahedi

Moosavi-Movahedi

et al. 2011

Journal of Dairy Research

134

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“…ACE activity assays were made at 340 nm and 37°C using a measuring time of 10 min. One unit (U) of ACE activity was expressed as the amount of the enzyme that will hydrolyze 1 µmol of the substrate FAPGG into FAP and glycyl-glycine in 1 min at 37°C ( 14 ). Normalized plasma ACE activity was determined at the end of the measurements, by dividing the ACE value to total plasma protein value for each animal.…”

Section: Methodsmentioning

confidence: 99%

Impact of chronic administration of anabolic androgenic steroids and taurine on blood pressure in rats

Roşca

Stoian

Badiu

et al. 2016

Braz J Med Biol Res

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Supraphysiological administration of anabolic androgenic steroids has been linked to increased blood pressure. The widely distributed amino acid taurine seems to be an effective depressor agent in drug-induced hypertension. The purpose of this study was to assess the impact of chronic high dose administration of nandrolone decanoate (DECA) and taurine on blood pressure in rats and to verify the potentially involved mechanisms. The study was conducted in 4 groups of 8 adult male Wistar rats, aged 14 weeks, treated for 12 weeks with: DECA (A group); vehicle (C group); taurine (T group), or with both drugs (AT group). Systolic blood pressure (SBP) was measured at the beginning of the study (SBP1), 2 (SBP2) and 3 months (SBP3) later. Plasma angiotensin-converting enzyme (ACE) activity and plasma end products of nitric oxide metabolism (NOx) were also determined. SBP3 and SBP2 were significantly increased compared to SBP1 only in the A group (P<0.002 for both). SBP2, SBP3 and ACE activity showed a statistically significant increase in the A vs C (P<0.005), andvs AT groups (P<0.05), while NOx was significantly decreased in the A and AT groups vs controls (P=0.01). ACE activity was strongly correlated with SBP3 in the A group (r=0.71, P=0.04). These findings suggest that oral supplementation of taurine may prevent the increase in SBP induced by DECA, an effect potentially mediated by angiotensin-converting enzyme.

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“…(FAPGG) (Sigma Diagnostic-305, St Louis, MO, USA) was used as a substrate according to the method described by Harjanne (16). Three determinations were performed from each serum sample and the mean result was chosen as the final value.…”

Section: Furiulacr Yloil-l-phenyl-alanil-glicilglicinementioning

confidence: 99%

Serum angiotensin converting enzyme and C4 protein of complement as a combined diagnostic index in alcoholic liver disease

Sopeña

Martínez-Vázquez

Fernández-Rodrı́guez

et al. 1996

Liver

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Diagnosis of liver cirrhosis relies on hepatic biopsy. So far, attempts have failed to achieve a serologic test that differentiates cirrhosis from other hepatic conditions. The aim of this work was to assess the diagnostic value of the ratio of serum angiotensin converting enzyme activity (SACE) and the levels of protein C4 of serum complement (SACE/C4) in differentiating cirrhotic from noncirrhotic alcoholic liver diseases. In this study, 68 active alcoholic patients (17 with fatty liver or minimal changes, 11 with acute alcoholic hepatitis and 40 with cirrhosis) were included. Twenty healthy subjects were studied as a control group. Liver biopsy was performed in all patients. SACE levels were significantly higher in the group with cirrhosis when compared with the group of patients without cirrhosis and the control. On the other hand, serum C4 level decreased as liver damage progressed. SACE values above 25 IU/l had a sensitivity of 92.5 percent (95 percent confidence interval, 87.5 to 97.5) and a specificity of 79 percent (95% percent confidence interval, 70.5 to 87.5), in detecting those patients with liver cirrhosis. The sensitivity further increased to 95 percent (95 percent confidence interval, 90.5 to 99.5) and the specificity to 100 percent when the SACE/C4 ratio was used and a cutoff point of 145 was chosen. To conclude, in alcoholics SACE is specifically elevated in patients with cirrhosis, and the SACEIC4 ratio is a excellent biochemical index for the diagnosis of cirrhosis in alcoholic I patients. Cupyright 0 Munksgaard 1996 LNER ISSN 0106.9543

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Automated kinetic determination of angiotensin-converting enzyme in serum.

Cited by 26 publications

References 0 publications

Biological activity of camel milk casein following enzymatic digestion

Biological activity of camel milk casein following enzymatic digestion

Impact of chronic administration of anabolic androgenic steroids and taurine on blood pressure in rats

Serum angiotensin converting enzyme and C4 protein of complement as a combined diagnostic index in alcoholic liver disease

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