Automated instrument for absorption-corrected molecular fluorescence measurements by the cell shift method

Christmann, D. R.; Crouch, S. R.; Timnick, Andrew.

doi:10.1021/ac00225a034

Cited by 28 publications

(9 citation statements)

References 22 publications

(16 reference statements)

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“…We also took into consideration that the red shift may have been due to re-absorption of the fluorescence emission since this is commonly observed in concentrated systems. Evidence of re-absorption usually comes in the form of relatively small Stoke's shifts, narrowing of the emission band as well as significant changes in the fluorescent lifetime of the fluorophore [28]. Further analysis of our data showed the opposite effect in all three cases.…”

Section: Intermolecular Interaction In the Solid Statementioning

confidence: 57%

The Role of Intermolecular Interactions in Solid State Fluorescent Conjugated Polymer Chemosensors

et al. 2011

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A functionalized fluorescent conjugated polymer, tolylterpyridine poly(p-phenyleneethynylene-thienyleneethynylene (ttp-PPETE), was designed and synthesized to detect trace amounts of toxic transition metal pollutants in ground water. Photophysical studies in tetrahydrofuran (THF) successfully demonstrated this polymer as a selective and sensitive chemosensor for Ni(2+) and Co(2+) in aqueous solution. Solid state composites of these chemosensors have now been prepared which can be modified to provide for inexpensive and portable field based chemical detection. A solid composite of ttp-PPETE, blended with poly (methyl methacrylate) shows UV-vis absorption and fluorescence emission spectra which are red- shifted when compared to solution phase spectra, suggesting an increase in conjugation in the solid state. An additional absorption peak, not present in solution, is also observed in the solid state. The presence of this new peak provides evidence of interacting FCP chains in the solid state. Concentration dependent experiments were done on the solid composite showing red-shifted emission peaks accompanied by a significant reduction in the fluorescent quantum yield. These observations are consistent with the formation of aggregated polymer species in the solid state. Intermolecular interactions of this type can be manipulated in the design of sensitive and selective solid state fluorescent conjugated polymer sensors.

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Section: Intermolecular Interaction In the Solid Statementioning

confidence: 57%

The Role of Intermolecular Interactions in Solid State Fluorescent Conjugated Polymer Chemosensors

et al. 2011

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“…In the 1970's and 1980's it was recognized that using a vibrating mirror [16,17], or shifting [18] or rotating [9,19] the cell could account for inner filter effects. Improvement of these methods, such as automating the cuvette translation [20][21][22], continued with Tucker et al publishing a related tutorial article [23] in 1992. Accounting for inner filter effects with alternative methods, such as Raman scattering [24], continue.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence measurements for evaluating the application of multivariate analysis techniques to optically thick environments.

Reichardt¹,

Timlin²,

Jones³

et al. 2010

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Laser-induced fluorescence measurements of cuvette-contained laser dye mixtures are made for evaluation of multivariate analysis techniques to optically thick environments. Nine mixtures of Coumarin 500 and Rhodamine 610 are analyzed, as well as the pure dyes. For each sample, the cuvette is positioned on a two-axis translation stage to allow the interrogation at different spatial locations, allowing the examination of both primary (absorption of the laser light) and secondary (absorption of the fluorescence) inner filter effects. In addition to these expected inner filter effects, we find evidence that a portion of the absorbed fluorescence is re-emitted. A total of 688 spectra are acquired for the evaluation of multivariate analysis approaches to account for nonlinear effects.

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“…Since the design of this prototype fluorescence detection system is different from our previous study, an analytical model based on Beer-Lambert law [16][17][18][19] is developed to predict the fluorescence intensity that increases with the amplification of DNA concentration during PCR thermal cycling in this prototype. The predicted values can be used as a basis for determining DNA initial copies in PCR mix and identifying the noise characters of the results that are obtained through a signal filtering algorithm over fluorescence images captured by the CCD detector.…”

Section: Introductionmentioning

confidence: 99%

Development of a CCD-based fluorimeter for real-time PCR machine

Lee

Chang

Chen

2005

Sensors and Actuators B: Chemical

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Automated instrument for absorption-corrected molecular fluorescence measurements by the cell shift method

Cited by 28 publications

References 22 publications

The Role of Intermolecular Interactions in Solid State Fluorescent Conjugated Polymer Chemosensors

The Role of Intermolecular Interactions in Solid State Fluorescent Conjugated Polymer Chemosensors

Fluorescence measurements for evaluating the application of multivariate analysis techniques to optically thick environments.

Development of a CCD-based fluorimeter for real-time PCR machine

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