Automated Immunonephelometric Assay for Human Alpha-1-Microglobulin

Lammers, Manfred; Gruber, Walter; Frese, Concepcion; Metzmann, Erwin

doi:10.1159/000418769

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“…In literature, the intra-and inter-assay precision of the di-microglobulin determination with an immunochemical method is given as 3-1 1% CV (13,14,16,27). This compares reasonably well with the between-run CV of 11 -16% of our method with use of the Behring standard.…”

Section: Discussionsupporting

confidence: 73%

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The Determination of α₁-Microglobulin by Means of an Automated Latex Immunoassay

Štraub

Baard²,

Jour³

et al. 1995

CCLM

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Summary: Polystyrene (latex) particles coated with human anti-aj-microglobulin antibodies are used in an automated "kinetic" assay for a r microglobuiin in urine. For values below 12 mg/1, there was no significant difference between two kinds of standard, but above 12 mg/1 the results depend on the origin of the ai-microglobulin standard.Correlation between values obtained with both standards was good (r 2 = 0.968). The method has a between-run imprecision (CV) of 11 -16%. Comparison with two commercial enzyme immunoassays gave a bias of -10% to + 40%, while two nephelometric methods differed by 0% to 23%, possibly because the standards used in these methods were of different origin. These results indicate the necessity of standardization of the determination of aimicroglobulin. The detection limit of our method was 0.8 mg/1, enabling the application of the method for epidemiological investigations.

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Section: Discussionsupporting

confidence: 73%

“…cti-Micrpglobulin has been determined with radial immunodiffiision (9,10), turbidimetric and nephelometric methods (11,12,13), and enzyme immunoassays (14)(15)(16)(17)(18). For an assay to be used in epidemiology, it is necessary that proteins and enzymes can be detected in urine of apparently healthy persons.…”

Section: Introductionmentioning

confidence: 99%

The Determination of α₁-Microglobulin by Means of an Automated Latex Immunoassay

Štraub

Baard²,

Jour³

et al. 1995

CCLM

View full text Add to dashboard Cite

show abstract

Automated Immunonephelometric Assay for Human Alpha-1-Microglobulin

Cited by 1 publication

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The Determination of α₁-Microglobulin by Means of an Automated Latex Immunoassay

The Determination of α₁-Microglobulin by Means of an Automated Latex Immunoassay

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Automated Immunonephelometric Assay for Human Alpha-1-Microglobulin

Cited by 1 publication

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The Determination of α1-Microglobulin by Means of an Automated Latex Immunoassay

The Determination of α1-Microglobulin by Means of an Automated Latex Immunoassay

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The Determination of α₁-Microglobulin by Means of an Automated Latex Immunoassay

The Determination of α₁-Microglobulin by Means of an Automated Latex Immunoassay