Automated, high-throughput DNA extraction protocol for disposable label free, microfluidics integrating DNA biosensor for oil palm pathogen, Ganoderma boninense

Nucleic Acid Extraction Methods and Technologies for On-Site Plant–Pathogen Diagnostics

“…Paul and his co-workers developed a polymer-based microneedle patch in combination with a real-time polymerase chain reaction (RT-PCR) approach for on-site diagnosis of potato late blight fungus in potato and tomato plants . Ayoib et al reported the limitations of conventional nucleic acid extraction methods and proposed on-chip DNA extraction tools as a convincing alternative that uses the physical advantages of microfluidics . Additionally, a loop-mediated isothermal amplification (LAMP) method provided successful nucleic acid amplifications using low-purity nucleic-acid samples.…”

“…21 Ayoib et al reported the limitations of conventional nucleic acid extraction methods and proposed on-chip DNA extraction tools as a convincing alternative that uses the physical advantages of microfluidics. 23 Additionally, a loop-mediated isothermal amplification (LAMP) method provided successful nucleic acid amplifications using low-purity nucleic-acid samples. Hence, development of a LAMP-based plant-pathogen detection technique accelerated the development of fieldbased nucleic acid extraction methods without requiring a laboratory environment and trained personnel.…”

Problems and Prospects of Improving Abiotic Stress Tolerance and Pathogen Resistance of Oil Palm

“…The detection technology of oil palm base rot mainly includes extraction methods and model establishment, which is conducive to early detection and control of the disease and guarantees the growth and development of oil palm. The DNA extraction method combined with a disposable microfluidic device and a biosensor realizes the automated, high-throughput, label-free detection of the pathogens of oil palm base rot [79]. There is a good correlation between ergosterol and the degree of infection of oil palm.…”

Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

“…Alternative to conventional methods, microfluidic systems offer miniaturization of lab-scale applications that allow operating at a few μL or even nL and achieving better accuracy, reduced analysis time, and higher reliability of the entire isolation process while minimizing the risk of cross-contamination. , The ability to precisely control fluid at the microscale has opened up numerous possibilities for replacing batch-top equipment with continuous flow processes and various functional components such as channels, valves, pumps, mixers, and sensors in microfluidic systems. − Over the recent years, microfluidic isolation of DNA has evolved from microcapillary chromatography columns , to microfluidic devices and platforms in various designs such as cartridges, , centrifugal devices (so-called lab-on-a-disc ), − glass, and polydimethylsiloxane (PDMS) microchips. − Microfluidic platforms incorporating surface-modified magnetic beads enable the selective purification of targeted DNA from other substances under the influence of a magnetic field created by electromagnets, , integrated soft magnets, , or external permanent magnets . Electromagnets flexibly control the magnitude and form the magnetic field; however, the heat generation due to the coil-carrying current results in a deterioration of the stability of the magnetic field, especially when strong magnetic fields are required .…”