Automated enzymatic measurement of adenosine deaminase isoenzyme activities in serum

Muraoka, Toshiharu; Katsuramaki, Tuneo; Shiraishi, Hiroaki; Yokoyama, Mineyuki

doi:10.1016/0003-2697(90)90455-i

Cited by 78 publications

(56 citation statements)

References 13 publications

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“…It has been demonstrated that the presence of these isoenzymes in serum, regardless of their amount, has a diagnostic value. Previous studies found the elevated activity of ADA2 in a variety of conditions such as acquired immunodeWciency syndrome (AIDS), T-cell lymphoblastic malignancies, tuberculosis, liver diseases, rheumatoid arthritis, and myasthenia gravis (MG) [6,[8][9][10][11][12][13][14]. We have recently reported the similar data for primary immunodeWciency disorders (PIDs) [7].…”

Section: Introductionmentioning

confidence: 57%

“…One unit of ADA is deWned as the amount of enzyme that converts 1 mM adenosine to inosine and ammonia per minute under standard assay conditions and is expressed as IU/l. The enzyme is stable for at least 24 h at 25°C, 7 days at 4°C, and 3 months at ¡20°C [26,28]. To minimize the measurement errors, all the assays were carried out three times and the mean value is presented.…”

Section: Ada Assaymentioning

confidence: 99%

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Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

et al. 2011

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Adenosine deaminase (ADA) plays a crucial role in the development and maintenance of normal immune system. So, any immunological imbalances could associate with its altered activity in serum. This study evaluated the activity of total ADA and its isoenzymes in serum of 45 patients with systemic lupus erythematosus (SLE). Included were 23 patients with active SLE, 22 during the inactive phase of the disease, and 45 healthy subjects. Our results provided evidence that the significantly elevated total ADA activity in serum of SLE patients is correlated mainly with the increased ADA2 level. The highest mean ADA2 activity during the relapse phase of the disease could be an indication to the macrophages, the main source of ADA2. It might be concluded that ADA and its isoenzymes analysis in serum of patients could be used as a useful and non-invasive diagnostic tool in evaluation of SLE active phase and the disease severity.

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Section: Introductionmentioning

confidence: 57%

Section: Ada Assaymentioning

confidence: 99%

Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

et al. 2011

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“…In addition, ADA2 has a lower optimal pH and is more resistant to inhibition by EHNAthan ADA1 or the ADA1 + CP isozyme (13). These properties have been utilized for the individual measurements of the total ADA,ADA1 and ADA2 activities (7).…”

Section: Discussionmentioning

confidence: 99%

Isozyme Analysis of the High Serum Adenosine Deaminase Activity in Patients with Myasthenia Gravis.

et al. 1995

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The serum activities ofadenosine deaminase (ADA) and its isozymes (ADA 1 and ADA2) were measured in 31 patients with myasthenia gravis (MG). As compared with 50 normal controls, in MGthe total activity and ADA1 were significantly high (p<0.01, respectively), and ADA2 tended to be high. The total activity and ADA2 were higher in generalized MGthan in ocular MG. ADA 2 was significantly high in grade IIB patients as compared with grade I patients divided by Osserman's classification (p<0.05). Patients with bulbar signs showed a significantly high total ADA(p<0.05) with an increasing tendency for ADA2. There was a significant elevation of the total activity and ADA2 in patients with positive anti-acetylcholine-receptor antibody as compared to those of the negative antibody (p<0.05). It was concluded that total ADA,reflecting the increase of both ADA1 and ADA2, is high in MG;and the measurement ofADA2 is more important because ADA2 was increased with advancing clinical MGgrade. (Internal Medicine 34: 81-84, 1995)

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“…The conversion of hydrogen peroxide by peroxidase into the quinone dyes 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine was then monitorized at 550 nm in a thermostatized spectrophotometer at 37 ° C (UVIKON 933 UV/Visible). ADA2 activity was measured by the same method, in the presence of 0.1 mM of erythro-9-(2-hydroxy-3-nonyl) adenine hydrochloride (EHNA, Sigma), a potent selective inhibitor of ADA1 [11]. ADA1 activity was then calculated by subtracting ADA2 activity from total ADA activity.…”

Section: Methodsmentioning

confidence: 99%

Adenosine Deaminase Two and Immunoglobulin M Accurately Differentiate Adult Sneddon’s Syndrome of Unknown Cause

et al. 2018

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Background: The association that exists between livedo reticularis (LR) and stroke is known as Sneddon’s syndrome (SnS). The disorder is classified as primary SnS (PSnS), if the cause remains unknown and secondary SnS. The condition is rare and it occurs mainly sporadically. In 2014, 2 independent teams described a new genetic disorder with childhood-onset, which was called deficiency of adenosine deaminase 2 (DADA2), characterized by recurrent fevers and vascular pathologic features that included LR and stroke. All the patients carried recessively inherited mutations in cat eye syndrome chromosome region candidate 1 gene (CECR1), encoding the adenosine deaminase 2 (ADA2) protein. Genetic testing is the standard for the diagnosis of DADA2. However, the diagnostic accuracy of more affordable laboratorial analysis in CECR1-mutated individuals remains to be established. We aim to determine whether plasma ADA2 activity and serum immunoglobulin M (IgM) levels can distinguish (1) DADA2 from other adult patients within the SnS spectrum, and (2) healthy CECR1 heterozygous (HHZ) from healthy controls (HC). Methods: ADA2 activity in plasma and serum IgM concentrations was measured in adult patients within the SnS spectrum, healthy first-degree relatives and HC. Genetic results were used as the reference standard. The primary outcome measures were sensitivity and specificity derived from receiver operating curve analysis. Results: A total of 73 participants were included in the study: 26 patients with PSnS with no CECR1 mutation (PSnS), 6 bi-allelic (DADA2 patients) and 7 HHZ CECR1 mutations and 34 HC. Plasma ADA2 activity and serum IgM levels were significantly lower in DADA2 patients than in PSnS. With the use of the best indexes, plasma ADA2 activity differentiated PSnS from DADA2 with a sensitivity and specificity of 100.0% and HHZ from HC with a sensitivity of 97.1% and specificity of 85.7%. Serum IgM levels also differentiated PSnS from DADA2 with a sensitivity of 85.2% and specificity of 83.3%. Conclusion: Serum IgM levels might be used as a triage tool and plasma ADA2 activity performs perfectly as a diagnostic test for DADA2 in adult patients within the SnS spectrum. ADA2 activity in plasma also reliably distinguishes HHZ from HC.

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Automated enzymatic measurement of adenosine deaminase isoenzyme activities in serum

Cited by 78 publications

References 13 publications

Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

Isozyme Analysis of the High Serum Adenosine Deaminase Activity in Patients with Myasthenia Gravis.

Adenosine Deaminase Two and Immunoglobulin M Accurately Differentiate Adult Sneddon’s Syndrome of Unknown Cause

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