Automated Design of Electron Mirrors for Multipass Electron Microscopy and 4D-STEM+EELS

Harvey, Tyler R.; Ophus, Colin

doi:10.1017/s1431927622011497

Cited by 1 publication

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…STEM SPA resolution can potentially be improved by phase retrieval methods [23,24]. Practical considerations constrain the combination of these methods with EELS but hold promise for the future [25][26][27][28]. Processing algorithms used in this work are standard SPA algorithms and therefore optimised for TEM phase contrast images.…”

Section: Discussionmentioning

confidence: 99%

Localising elements in single-particle reconstructions by REEL-EM: Reconstructed Electron Energy-Loss - Elemental Mapping

Pfeil-Gardiner,

Rosa,

Riedel

et al. 2024

Preprint

View full text Add to dashboard Cite

For structures determined by single particle cryo-EM, no technique currently exists for mapping elements to defined locations, leading to errors in the assignment of metals and other ions, cofactors, substrates, inhibitors, and lipids that play essential roles in activity and regulation. Elemental mapping in the electron microscope is well established for dose-tolerant samples but is challenging for biological samples, especially in a cryo-preserved state. Here, we combine electron energy-loss spectroscopy (EELS) with single-particle image processing to allow elemental mapping in cryo-preserved macromolecular complexes. Proof-of-principle data show that our method, REEL-EM, allows 3D reconstruction of EELS data, such that a high total electron dose is accumulated across many copies of a complex. Working with two test samples, we demonstrate that we can reliably localise abundant elements. We discuss the current limitations of the method and potential future developments.

show abstract