Automated Counting of Daphnid Neonates, <i>Artemia</i> Nauplii, and Zebrafish Eggs: A Proof of Concept

Abreu, S. N.; Jesus, Fátima; Domingues, Inês; Batista, Filipa; Pereira, Joana Luísa; Serpa, Dalila; Soares, Amadeu M.V.M.; Martins, Rui Escadas; Silva, Miguel Oliveira e

doi:10.1002/etc.5323

Cited by 3 publications

(1 citation statement)

References 22 publications

(21 reference statements)

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“…Ecotoxicology testing using in vivo assays is, in general, labour intensive, whereby the manipulation of organisms is mainly manual, which may increase data variability and decrease reproducibility. In this way, microfluidics automation of organisms sorting, collection and positioning and chemical addition and dilution, combined with powerful data collection and analysis tools offer a significant upgrade to ecotoxicological tests (Campana and Wlodkowic, 2018a;Abreu et al, 2022). However, there are also some limitations for its development, such as organism size, which is an important parameter to the development of micro/millifluidic devices, currently limited to organisms < mm in size (Campana and Wlodkowic, 2018b).…”

Section: Innovative Approaches To Assessing Nms Toxicity Using Daphni...mentioning

confidence: 99%

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

et al. 2023

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The importance of the cladoceran Daphnia as a model organism for ecotoxicity testing has been well-established since the 1980s. Daphnia have been increasingly used in standardised testing of chemicals as they are well characterised and show sensitivity to pollutants, making them an essential indicator species for environmental stress. The mapping of the genomes of D. pulex in 2012 and D. magna in 2017 further consolidated their utility for ecotoxicity testing, including demonstrating the responsiveness of the Daphnia genome to environmental stressors. The short lifecycle and parthenogenetic reproduction make Daphnia useful for assessment of developmental toxicity and adaption to stress. The emergence of nanomaterials (NMs) and their safety assessment has introduced some challenges to the use of standard toxicity tests which were developed for soluble chemicals. NMs have enormous reactive surface areas resulting in dynamic interactions with dissolved organic carbon, proteins and other biomolecules in their surroundings leading to a myriad of physical, chemical, biological, and macromolecular transformations of the NMs and thus changes in their bioavailability to, and impacts on, daphnids. However, NM safety assessments are also driving innovations in our approaches to toxicity testing, for both chemicals and other emerging contaminants such as microplastics (MPs). These advances include establishing more realistic environmental exposures via medium composition tuning including pre-conditioning by the organisms to provide relevant biomolecules as background, development of microfluidics approaches to mimic environmental flow conditions typical in streams, utilisation of field daphnids cultured in the lab to assess adaption and impacts of pre-exposure to pollution gradients, and of course development of mechanistic insights to connect the first encounter with NMs or MPs to an adverse outcome, via the key events in an adverse outcome pathway. Insights into these developments are presented below to inspire further advances and utilisation of these important organisms as part of an overall environmental risk assessment of NMs and MPs impacts, including in mixture exposure scenarios.

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Section: Innovative Approaches To Assessing Nms Toxicity Using Daphni...mentioning

confidence: 99%

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

et al. 2023

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Using channel pruning–based YOLOv5 deep learning algorithm for accurately counting fish fry in real time

Xu,

Chen,

et al. 2024

Aquacult Int

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A Compact and Efficient Brine Shrimp Hatching Device

Cardoso

2023

BIOT

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Background: Artemia is considered an indispensable live food for rearing many fish and crustacean larvae and can be used in toxicity tests as a safe, practical, and economical method. It is straightforward to create and manipulate; however, many cysts do not hatch as expected. Methods: A new device was used to hatch Artemia salina. The device includes a tubular-shaped reservoir, lid with a central hole, heater with a thermostat, air compressor, three porous stones, funnel-shaped bottom, light source, collection valve, switch, and electrical outlet, which permits better control of temperature and aeration in a single apparatus. Different devices were used for comparative analysis. Results: Hatched eggs of Artemia showed that the device was 97% efficient; in comparison, commercial and other devices hatched approximately less than 80% of the cysts. Although Artemia is an easy-to-handle animal, the device described here facilitates changes in the growth parameters and produces more nauplii larvae. Conclusion: This paper presents a new device that is effective, compact, and successful for hatching brine shrimp cysts, thereby improving Artemia production.

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Automated Counting of Daphnid Neonates, Artemia Nauplii, and Zebrafish Eggs: A Proof of Concept

Cited by 3 publications

References 22 publications

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

Using channel pruning–based YOLOv5 deep learning algorithm for accurately counting fish fry in real time

A Compact and Efficient Brine Shrimp Hatching Device

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