Automated cellular stimulation with integrated pneumatic valves and fluidic capacitors

Adeoye, Damilola I.; Wang, Yao; Davis, Joshua J.; Roper, Michael G.

doi:10.1039/d2an01985j

Cited by 2 publications

(1 citation statement)

References 40 publications

(67 reference statements)

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“…These data suggest that islets showing a double peak are metabolically more active, perhaps reflecting islet functional heterogeneity. Such double-peak responses can be sporadically found in the data presented by other groups, 44 , 45 , 46 but we are the first to highlight the pattern and show that it is glucose dependent. To investigate the metabolism underlying a double-peak response, we took advantage of the optical window underneath the islets to simultaneously measure GSIS ( Figures 6 D–6G) while imaging MMP using TMRE ( Figures 6 D and 6E) and ATP using PercevalHR ( Figures 6 F and 6G).…”

Section: Resultssupporting

confidence: 64%

Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets

Wang,

Regeenes,

Memon

et al. 2023

Cell Reports Methods

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Section: Resultssupporting

confidence: 64%

Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets

Wang,

Regeenes,

Memon

et al. 2023

Cell Reports Methods

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Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon

Wang,

Skinner,

Roper

2024

Electrophoresis

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Glucagon plays a crucial role in regulating glucose homeostasis; unfortunately, the mechanisms controlling its release are still unclear. Capillary electrophoresis (CE)‐ and fluorescence anisotropy (FA)‐immunoassays (IA) have been used for online measurements of hormone secretion on microfluidic platforms, although their use in glucagon assays is less common. We set out to compare a glucagon‐competitive IA using these two techniques. Theoretical calibration curves were generated for both CE‐ and FA–IA and results indicated that CE‐IA provided higher sensitivity than FA–IA. These results were confirmed in an experiment where both assays showed limits of detection (LOD) of 30 nM, but the CE‐IA had ∼300‐fold larger sensitivity from 0 to 200 nM glucagon. However, in online experiments where reagents were mixed within the device, the sensitivity of the CE‐IA was reduced ∼3‐fold resulting in a higher LOD of 70 nM, whereas the FA–IA remained essentially unchanged. This lowered sensitivity in the online CE‐IA was likely due to poor sampling by electroosmotic flow from the high salt solution necessary in online experiments, whereas pressure‐based sampling used in FA–IA was not affected. We conclude that FA–IA, despite lowered sensitivity, is more suitable for online mixing scenarios due to the ability to use pressure‐driven flow and other practical advantages such as the use of larger channels.

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Automated cellular stimulation with integrated pneumatic valves and fluidic capacitors

Abstract: To address flow control challenges in microfluidic systems with vacuum-driven flow, integrated valves and capacitors were used to deliver smooth stimulant profiles to murine islets of Langerhans for dynamic insulin secretion measurements.

Cited by 2 publications

References 40 publications

Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets

Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets

Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon

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