Automated Cell Separation Techniques Based on Optical Trapping

Flow Cytometry

“…This discussion follows that presented in Buican (1991). Following this, we discuss the applications of two-dimensional (2-D) and three-dimensional (3-D) optical traps in cell separation.…”

“…View of the laser sorter manipulation chamber (see Figure 4 for the geometry of the beams inside the chamber). (Reproduced with permission from Buican (1991), Copyright 1991 Am. The elution and rinsing ports are used to create local fluid flows near the side windows.…”

“…Conservation of momentum in a light scattering system: B o ' BI-incident and scattered beams; S-scattering object; Po, PI-momentum fluxes of the two beams; Flight pressure force due to scattering of the incident beam by S. (Reproduced with permission fromBuican (1991), Copyright 1991 …”

“…(Reproduced with permission fromBuican (1991), Copyright 1991 The trapped cell is carried along a separation path that avoids other cells on the bottom of the manipulation chamber.…”

“…(Reproduced with permission fromBuican (1991), Copyright 1991 Am. :entral processing unit; FG-frame grabber; L-imaging lens; M-mirror; O-microscope objective; V-video camera; S-xyz stage with servo actuators.…”