Automated Buildup of Biomimetic Films in Cell Culture Microplates for High‐Throughput Screening of Cellular Behaviors

Machillot, Paul; Quintal, Catarina; Dalonneau, Fabien; Hermant, Loïc; Monnot, Pauline; Matthews, Kelsey; Fitzpatrick, Veronica; Liu, Jie; Pignot-Paintrand, Isabelle; Picart, Catherine

doi:10.1002/adma.201801097

Cited by 39 publications

(54 citation statements)

References 41 publications

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“…Particularly, LbL technology presented several advantages for biomedicine: (i) deposition of homogeneous films with controlled thickness, (ii) high loading capacities and controlled release of biomolecules/drugs of various nature, and (iii) coating stability under physiological conditions. This made the LbL method one of the most rapidly growing strategies for generating thin film coatings of biomedical scaffolds [ 3 , 4 , 5 , 6 ], patterned surfaces [ 7 , 8 ], medical devices [ 9 , 10 ], implants [ 11 , 12 ], and a range of alternate bioapplications ( Figure 1 ); while multilayer capsules became promising nano- and micro-carriers for drug delivery applications [ 1 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Biopolymer-based Multilayersmentioning

confidence: 99%

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Campbell

Vikulina

2020

Polymers

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Rapid development of versatile layer-by-layer technology has resulted in important breakthroughs in the understanding of the nature of molecular interactions in multilayer assemblies made of polyelectrolytes. Nowadays, polyelectrolyte multilayers (PEM) are considered to be non-equilibrium and highly dynamic structures. High interest in biomedical applications of PEMs has attracted attention to PEMs made of biopolymers. Recent studies suggest that biopolymer dynamics determines the fate and the properties of such PEMs; however, deciphering, predicting and controlling the dynamics of polymers remains a challenge. This review brings together the up-to-date knowledge of the role of molecular dynamics in multilayers assembled from biopolymers. We discuss how molecular dynamics determines the properties of these PEMs from the nano to the macro scale, focusing on its role in PEM formation and non-enzymatic degradation. We summarize the factors allowing the control of molecular dynamics within PEMs, and therefore to tailor polymer multilayers on demand.

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Section: Biopolymer-based Multilayersmentioning

confidence: 99%

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Campbell

Vikulina

2020

Polymers

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“…Cell area was analyzed by measuring the actin positive area with Analyze Particles (Image J) [56]. The total area of all cells in 1 image was divided by the total number of nuclei, counted as described above, to reduce the influence of overlapping cells [57].…”

Section: Image Analysis Homogeneity Analysis and Custom-made Macrosmentioning

confidence: 99%

“…To stain ALP, we used fast blue RR salt in a 0.01 % (w/v) naphthol AS-MX solution (Sigma Aldrich) according to the manufacturer's instructions. ALP staining was quantified in dry conditions by scanning the 96-well plate with a scanner (V600 photo, EPSON, Seiko-Epson Corporation), and gray-scale images were acquired at a resolution of 1200 dpi [57]. The images (8-bit) were analyzed with Image J.…”

Section: Alkaline Phosphatase (Alp) Stainingmentioning

confidence: 99%

Heparan sulfate co-immobilized with cRGD ligands and BMP2 on biomimetic platforms promotes BMP2-mediated osteogenic differentiation

et al. 2020

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“…For unbiased screening and validation of immune-instructive biomaterials, high throughput methods such as microfluidics devices are extremely valuable, as they can be standardized and are more cost-effective. [366,367] As a matter of fact, in recent years, several high content analysis approaches have been developed to screen therapeutics for the induction of ROS and NET production, [368,369] and could be readily applied for testing of the neutrophil compatibility with biomaterials. Another example of an available high throughput approach is the TopoChip platform, which allows mathematically defined surface topographies to be screened for their influence on cellular responses.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Combating Implant Infections: Shifting Focus from Bacteria to Host

et al. 2020

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commensal skin bacteria, Staphylococci, and in particular Staphylococcus aureus, have a strong tendency to colonize foreign bodies and cause IAI. [3,4] Important for the underlying pathophysiology, Staphylococci are highly competent at producing biofilms on implant surfaces, which encapsulate the bacterial niche from the outside environment, [5,6] thereby protecting the bacteria from host defense systems and antibiotics. [7] Antibiotics are administered as a routine procedure. [8] However, as a consequence of widespread antibiotics usage, bacteria are exposed to subinhibitory concentrations of antibiotics at a larger scale, driving their development toward antibiotic resistance, [9] as illustrated by the emergence of methicillin-resistant S. aureus (MRSA). [10,11] As an improvement over current clinically applied local antibiotics delivery systems, [12] the recent developments in implant surface engineering approaches allow better antimicrobial functionalities to be incorporated to allow for more tunable and controlled drug release. [13-15] The "race to the surface" model is popular among biomaterials researchers to predict the biomaterials fate, resulting from the competition between eukaryotic cells and bacteria at the material surface. [16] Using this template, implant antibacterial properties are being stemmed from direct contact killing mechanisms due to implant surface modifications [17,18] or firm immobilization of drugs, [19,20] as they both "shield" the implant from bacterial adhesion. The current anti-infective biomaterials strategies being explored can be categorized as: 1) implant functionalization with antibiotics or antibacterial drugs such as host defense peptides (HDPs), inorganic materials (e.g., chitosan and derivatives), and inorganics (e.g., silver, copper, and zinc metal nanoparticles (NPs)), 2) anti-biofilm surface modification (e.g., coating with antifouling polymers or quorum sensor inhibitors), or 3) physical surface changes for direct contact-killing properties (e.g., nanotubes, nanopillars, and metal implantation). [15,21] Emerging as a serious alternative or adjuvant therapy to antibiotics, bacteriophage (phage) therapy uses viruses responsible for the lysis of specific bacterial strains. [22,23] As a natural predator of bacteria, phages employ different killing mechanisms, making multidrug resistant bacteria susceptible for phages. [24] Moreover, phages are highly specific for pathogenic cells, which can eliminate disastrous off-target effects in the host. [25] As a limitation, the use of phage cocktails is needed for therapeutic efficacy, [26] while there is currently is no conclusive data on possible long-term side effects of phage therapy in The widespread use of biomaterials to support or replace body parts is increasingly threatened by the risk of implant-associated infections. In the quest for finding novel anti-infective biomaterials, there generally has been a one-sided focus on biomaterials with direct antibacterial properties, which leads to excessive use of antibacterial ag...

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Automated Buildup of Biomimetic Films in Cell Culture Microplates for High‐Throughput Screening of Cellular Behaviors

Cited by 39 publications

References 41 publications

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Heparan sulfate co-immobilized with cRGD ligands and BMP2 on biomimetic platforms promotes BMP2-mediated osteogenic differentiation

Combating Implant Infections: Shifting Focus from Bacteria to Host

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