Automated Blood Cultures

Han, Xiang Y.

doi:10.1007/0-387-32892-0_1

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Automated blood culturing systems have improved the practice of blood culture: the automated system automatically detects microbial growth by monitoring microbial CO 2 detection, and eliminates the need for manual inspection or examination. In addition, growth of aerobes and facultative anaerobes are promoted by agitating culture bottles 104 . However, a recent review reported that many studies from LMIC used manual culture methods, with lower GBS incidence rates compared to studies using automated culture methods 105 .…”

Section: Diagnosis and Treatment Of Gbs Diseasementioning

confidence: 99%

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries

et al. 2016

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Globally, group B Streptococcus (GBS) remains the leading cause of sepsis and meningitis in young infants, with its greatest burden in the first 90 days of life. Intrapartum antibiotic prophylaxis (IAP) for women at risk of transmitting GBS to their newborns has been effective in reducing, but not eliminating, the young infant GBS disease burden in many high income countries. However, identification of women at risk and administration of IAP is very difficult in many low and middle income country (LMIC) settings, and is not possible for home deliveries. Immunization of pregnant women with a GBS vaccine represents an alternate pathway to protecting newborns from GBS disease, through the transplacental antibody transfer to the fetus in utero. This approach to prevent GBS disease in young infants is currently under development, and is approaching late stage clinical evaluation. This manuscript includes a review of the natural history of the disease, global disease burden estimates, diagnosis and existing control options in different settings, the biological rationale for a vaccine including previous supportive studies, analysis of current candidates in development, possible correlates of protection and current status of immunogenicity assays. Future potential vaccine development pathways to licensure and use in LMICs, trial design and implementation options are discussed, with the objective to provide a basis for reflection, rather than recommendations.

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Section: Diagnosis and Treatment Of Gbs Diseasementioning

confidence: 99%

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries

et al. 2016

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A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

Singh

Upadhyay

Sharma

et al. 2016

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Bacterial infections continue to be a major cause of deaths globally, particularly in resource-poor settings. In the absence of rapid and affordable diagnostic solutions, patients are mostly administered broad spectrum antibiotics leading to antibiotics resistance and poor recovery. Culture diagnosis continues to be a gold standard for diagnosis of bacterial infection, despite its long turnaround time of 24 to 48 h. We have developed a portable immunomagnetic cell capture (iMC(2)) system that allows rapid culture diagnosis of bacterial pathogens. Our approach involves the culture growth of the blood samples in broth media for 6 to 8 h, followed by immunomagnetic enrichment of the target cells using the iMC(2) device. The device comprises a disposable capture chip that has two chambers of 5 ml and 50 μl volume connected through a channel with a manual valve. Bacterial cells bound to antibody coated magnetic nanoparticles are swept from the 5 ml sample chamber into the 50 μl recovery chamber by moving an external magnetic field with respect to the capture chip using a linear positioner. This enables specific isolation and up to 100× enrichment of the target cells. The presence of bacteria in the recovered sample is confirmed visually using a lateral flow immunoassay. The system is demonstrated in buffer and blood samples spiked with S. typhi. The method has high sensitivity (10 CFU ml(-1)), specificity and a rapid turnaround time of less than 7 h, a significant improvement over conventional methods.

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Automated Blood Cultures

Cited by 2 publications

References 28 publications

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries

A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

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