Automated assay of N-acetyl-β-GLucosaminidase in normal and pathological human urine

Tucker, Susan; Boyd, Penelope J; Thompson, A. E.; Price, Robert G.

doi:10.1016/0009-8981(75)90245-4

Cited by 170 publications

(46 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, by including the automated measurement öf N-acetyl-ß-Dglucosaminidase and creatinine, the present profile yields rriPre Information than previously süggested regimes. The automated assay of N-acetyl-ß-D-glucpsaminidase was proposed äs a screening procedure by fucker et al (21).…”

Section: Discussionmentioning

confidence: 99%

“…This may however limit the diagnostic value in situ- Albumin 28 39 oti-Microglobulin 26 41 N-Acetyl-;ß-£>-glucosaminidase 28 Oi-Microglobulin 21 7 N-AcetyUß-i)-glucosaminidase 20 8 Protein test strip 12 16 2. N-Acetyl-ß-Z>-glucosaminidase > 0.56 kU/mol creatinine (5 U/g creatinine) n = 28 -Microglobulm 24 12 N-Acetyl-ß-i)-glucosarainidase 28 10 Protein test strip 21 Remarkably the biological variability for iSf-acetyl-ß-£)-glucosaminidase was only half of thät öf the other proteins. This might be explained by the fact thät IgG, albumin and a^microglobulin are filtered in varying amoünts through the glomerülar basal mem-brane.…”

Section: Biological Variation Of Urinary Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

A Diagnostic Programme for Quantitative Analysis of Proteinuria

Hofmann

Guder²

1989

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Summary:A spectrum of quantitative methods was adapted to the Kone Specific Analyser for the purpose of recognizing, quantifying and differentiating various forms of proteinuria. Total protein, IgG, albumin and oci-microglobulin (measured by turbidimetry), N-acetyl-ß-.D-glucosaminidase activity and creatinine (measured photometrically), were measured in undiluted urine; in addition a r microglobulin was measured in serum.Within and between run predsion, accuracy and linearity of the turbidimetric methods were in good agreement with nephelometric procedures. All turbidimetric methods exhibited a correlation coefficient r > 0.98 when compared with the radial immunodiffusion procedure äs reference method. Total protein measured turbidimetrically with the Kone Specific Analyser was in good agreement with the manual biuret procedure.The low detection limits and linearities allowed quantification of urine analytes from the lower ränge of normals up to ten times the upper limit of normals.The measured analytes exhibited stability in urine at pH 4-8 over at least seven days at 4 -6 °C and -20 °C. Only IgG showed a significant loss (up to 30 percent), when measured after storage at -20 °C.Quantities per mol creatinine showed significantly lower intra-individual and inter-individual variability than quantities per liter. In 31 normal persons, the intraindividual Variation was lowest for N-acetyl-ß-Z>-glucosaminidase activity (13%) and highest for total protein (33%), when measured in the second morning urine on 5 consecutive days.When related to creatinine, resülts obtäined in the second morning urine showed no significant differences from those in 24 h urine, except for aj-microglobulin which gave lower values in 24 h urines.The upper normal limits, calculated äs the 95% ranges, were determined from 154 urines of 31 individuals. Nearly all analytes showed an asymmetric distribution. Because of a wide tailing of the upper limit, preliminary üpper normal limits were set aböve this ränge:Total protein: 7.9 g/mol creatinine (70 mg/g creatinine) IgG:1.13 g/mol creatinine (10 mg/g creatinine) Albumin:2.26 g/mol creatinine (20 mg/g creatinine) ocrMicroglobulin: " 1.58 g/mol creatinine (14 mg/g creatinine) N^Acetyl-ß-jD-glücosaminidäse: 0.56 kU/mol creatinine (5 U/g creatinine).Application of the newly adapted programme to unselected urines sent for urine analysis revealed a threefold incfease in the propörtion of resülts outside the normal ranges, compared with the routinely used protein test strip procedure. All additional positive urines exhibited either signs of glomerular or tubular proteinuria. Determination of albumin or N-acetyl-ß-jD-glucosaminidase excretion was sufficient to detect these additional cases.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Biological Variation Of Urinary Proteinsmentioning

confidence: 99%

A Diagnostic Programme for Quantitative Analysis of Proteinuria

Hofmann

Guder²

1989

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…(Tucker et al, 1975) -a measure of the extent of renal damage -were measured on urine aliquots from 0-2, 2-4, 4-6, 6-8, 8-12 and 12-24h on days 1-4 inclusive. The first 12 h urine from day 5 was pooled and the same tests performed on an aliquot.…”

Section: Methodsmentioning

confidence: 99%

A pharmacokinetic and tolerance study of Ro13‐9904, a new cephalosporin antibiotic.

Pickup¹,

Bird²,

Lowe³

et al. 1981

Brit J Clinical Pharma

View full text Add to dashboard Cite

1 Six healthy male volunteers received a total of 2500 mg of a new cephalosporin antibiotic Ro13‐9904 by intramuscular injection in five divided doses at intervals of 12 h. 2 No significant systemic side‐ effects were observed and this was confirmed haematologically and biochemically. 3 The drug was distributed following intramuscular injection reaching a mean peak plasma concentration of 55 micrograms ml‐ 1 (range 46‐66) 1 to 2 h after the first injection. 4 Monoexponential elimination of drug was demonstrated. No significant difference was recorded in the plasma half‐life after the initial dose (mean 6.7 h) and at steady state (mean 6.7 h). The half‐life is long compared with other cephalosporin antibiotics. 5 On the basis of the observed half‐ life, steady state should be reached within 48 h. A mean peak plasma concentration of 74 micrograms ml‐1 (range 65‐87) was recorded at steady state. Steady state plasma concentrations of Ro13‐9904 with a dose of 500 mg every 13 h may be predicted from the pharmacokinetics of a single dose.

show abstract

“…Scand J Work Environ Health 1992, vol 18, no 4 Tucker et al (17), respectively. Lactate dehydrogenase in urine was assayed by the UV-test (Sevac, Czechoslovakia).…”

Section: Characteristicmentioning

confidence: 99%