Automated antinuclear immunofluorescence antibody screening: A comparative study of six computer-aided diagnostic systems

Bizzaro, Nicola; Antico, Antonio; Platzgummer, Stefan; Tonutti, Elio; Bassetti, D; Pesente, Fiorenza; Tozzoli, Renato; Tampoia, Marilina; Villalta, Danilo

doi:10.1016/j.autrev.2013.10.015

Cited by 110 publications

(102 citation statements)

References 26 publications

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“…Evaluation of the CyclopusCAD performance in negative/positive discrimination revealed comparable characteristics with commercialized systems (Table 5) [3]. Besides, in the present study the 17 sera that were falsely considered as ANA-positive had a cytoplasmic pattern.…”

Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 61%

“…These overall performances are close to that of EuroPattern® system, which was estimated at 79% [3]. While, it may surpass those of the three other systems, Aklides® (52%), NovaView® (54%) and G-Sight® (63%) [3]. It is worth to note that in the comparative study of these commercialized systems used only single pattern sera [3].…”

Section: Discussionmentioning

confidence: 80%

“…While, it may surpass those of the three other systems, Aklides® (52%), NovaView® (54%) and G-Sight® (63%) [3]. It is worth to note that in the comparative study of these commercialized systems used only single pattern sera [3]. In another study, including sera with mixed pattern, the global performance of the EuroPattern® system was estimated at 48.9% [7].…”

Section: Discussionmentioning

confidence: 99%

“…Different systems are in development and, to our knowledges, those currently proposed for routine analysis with image interpretation are [3]:…”

mentioning

confidence: 99%

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Comparative Study of Human and Automated Screening for Antinuclear Antibodies by Immunofluorescence on HEp-2 Cells

Gorgi¹,

Dhaouadi

Sfar

et al. 2015

Int. J. Stats. Med. Res.

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Abstract:Background: Several automated systems had been developed in order to reduce inter-observer variability in indirect immunofluorescence (IIF) interpretation. We aimed to evaluate the performance of a processing system in antinuclear antibodies (ANA) screening on HEp-2 cells. Patients and Methods:This study included 64 ANA-positive sera and 107 ANA-negative sera that underwent IIF on two commercial kits of HEp-2 cells (BioSystems® and Euroimmun®). IIF results were compared with a novel automated interpretation system, the "CyclopusCADImmuno®" (CAD).Results: All ANA-positive sera images were recognized as positive by CAD (sensitivity = 100%), while 17 (15.9%) of the ANA-negative sera images were interpreted as positive (specificity = 84.1%), =0.799 (SD=0.045). Comparison of IIF pattern determination between human and CAD system revealed on HEp-2 (BioSystems®), a complete concordance in 6 (9.37%) sera, a partial concordance (sharing of at least 1 pattern) in 42 (65.6%) cases and in 16 (25%) sera the pattern interpretation was discordant. Similarly, on HEp-2 (Euroimmun®) the concordance in pattern interpretation was total in 5 (7.8%) sera, partial in 39 (60.9%) and absent in 20 (31.25%). For both tested HEp-2 cells kits agreement was enhanced for the most common patterns, homogenous, fine speckled and coarse speckled. While there was an issue in identification of nucleolar, dots and nuclear membranous patterns by CAD. Conclusion:Assessment of ANA by IIF on HEp-2 cells using the automated interpretation system, the "CyclopusCADImmuno®" is a reliable method for positive/negative differentiation. Continuous integration of IIF images would improve the pattern identification by the CAD.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 61%