2024
DOI: 10.1364/boe.531700
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Automated analysis of scattering-based light sheet microscopy images of anal squamous intraepithelial lesions

Yongjun Kim,
Jingwei Zhao,
Brooke Liang
et al.

Abstract: We developed an algorithm for automatically analyzing scattering-based light sheet microscopy (sLSM) images of anal squamous intraepithelial lesions. We developed a method for automatically segmenting sLSM images for nuclei and calculating seven features: nuclear intensity, intensity slope as a function of depth, nuclear-to-nuclear distance, nuclear-to-cytoplasm ratio, cell density, nuclear area, and proportion of pixels corresponding to nuclei. 187 images from 80 anal biopsies were used for feature analysis a… Show more

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