Automated analysis of mitochondrial dimensions in mesenchymal stem cells: Current methods and future perspectives

Summer, Sabrina; Kocsis, Ágnes Katalin; Reihs, Eva I.; Rothbauer, Mario; Lonhus, Kirill; Štys, Dalibor; Ertl, Peter; Fischer, Michael B.

doi:10.1016/j.heliyon.2023.e12987

Cited by 4 publications

(1 citation statement)

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“…Nevertheless, further investigations are required to determine the optimal dosage, packaging, delivery methods, and ethical considerations associated with using isolated mitochondria. Furthermore, the integration of advanced imaging techniques and omics technologies has yielded valuable insights into the dynamic changes occurring within mitochondria during the differentiation of MSCs [122]. Systemic approaches, such as live detection on mitochondrial biogenesis and dynamics, in-depth profiling of mitochondrial metabolic and biochemical status, and rigorous mapping of mtDNA genome and mitochondrial protein heterogeneity, will enable the comprehensive evaluation of mitochondrial changes under different conditions.…”

Section: Strategies Gaps and Directions In Mitochondria-mediated Regu...mentioning

confidence: 99%

Mitochondria in Multi-Directional Differentiation of Dental-Derived Mesenchymal Stem Cells

Liu,

Xu,

et al. 2023

Biomolecules

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The pursuit of tissue regeneration has fueled decades of research in regenerative medicine. Among the numerous types of mesenchymal stem cells (MSCs), dental-derived mesenchymal stem cells (DMSCs) have recently emerged as a particularly promising candidate for tissue repair and regeneration. In recent years, evidence has highlighted the pivotal role of mitochondria in directing and orchestrating the differentiation processes of DMSCs. Beyond mitochondrial energy metabolism, the multifaceted functions of mitochondria are governed by the mitochondrial quality control (MQC) system, encompassing biogenesis, autophagy, and dynamics. Notably, mitochondrial energy metabolism not only governs the decision to differentiate but also exerts a substantial influence on the determination of differentiation directions. Furthermore, the MQC system exerts a nuanced impact on the differentiation of DMSCs by finely regulating the quality and mass of mitochondria. The review aims to provide a comprehensive overview of the regulatory mechanisms governing the multi-directional differentiation of DMSCs, mediated by both mitochondrial energy metabolism and the MQC system. We also focus on a new idea based on the analysis of data from many research groups never considered before, namely, DMSC-based regenerative medicine applications.

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Section: Strategies Gaps and Directions In Mitochondria-mediated Regu...mentioning

confidence: 99%