AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples*

Abstract

The AutoMate Express™ Forensic DNA Extraction System was developed for automatic isolation of DNA from a variety of forensic biological samples. The performance of the system was investigated using a wide range of biological samples. Depending on the sample type, either PrepFiler™ lysis buffer or PrepFiler BTA™ lysis buffer was used to lyse the samples. After lysis and removal of the substrate using LySep™ column, the lysate in the sample tubes were loaded onto AutoMate Express™ instrument and DNA was extracted using one of the two instrument extraction protocols. Our study showed that DNA was recovered from as little as 0.025 μL of blood. DNA extracted from casework-type samples was free of detectable PCR inhibitors and the short tandem repeat profiles were complete, conclusive, and devoid of any PCR artifacts. The system also showed consistent performance from day-to-day operation.

Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains

“…). In general, the DNA yields in this study are comparable or higher than reported for similar skeletal remains in previous studies (). Variation in DNA quantity between the technical replicates was observed (Table ).…”

“…DNA extraction procedures can be labor-intensive, time-consuming, include toxic chemicals, and be relatively inefficient (4,5). Over the past two decades, several DNA purification methods have developed as faster, simpler, and more effective kit-based technologies with the potential for automation (6)(7)(8)(9)(10)(11)(12)(13). However, despite some advancement in commercial DNA extraction kits, DNA extraction is still relatively time-consuming for skeletal remains due to lengthy sample preparation including cleaning, processing, and the requirement for crushing bone into a fine powder prior to digestion (4,5).…”

“…Bone preparation consists of cleaning the external surface of the bone (via washing, UV radiation, and/or sanding), cutting the bone into smaller pieces (if necessary), and crushing the bone into a powder with liquid nitrogen in a blender cup or a freezer mill (4,5,(14)(15)(16)(17)(18). While finely ground bone powder generally yields type-able amounts of DNA (4,5,7,12,13,(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28), it destroys the sample, increases the risk of contamination, and creates a potential hazard to the processor from airborne powder (4,17,27). Therefore, several studies have explored nondestructive extraction methods from whole bone and teeth (14,16,23,27).…”

A direct qPCR method for residual DNA quantification in monoclonal antibody drugs produced in CHO cells

“…Finally, 32 process intermediate samples of the four drug substances mentioned above were tested by the direct resDNA qPCR method and the residual DNA results were compared with the results obtained from a method using an automated DNA extractor called AutoMate Express (AExp) [9] followed by qPCR (Fig. 1).…”

Protocolo unificado de digestión y descalcificación de tres métodos de extracción de ADN de restos humanos

“…Los resultados indican que la molécula conservó sus propiedades incluso después de 24 horas en la solución tampón de digestión, es decir que la composición de dicha solución, la temperatura de 56 °C y el tiempo, posibilitaron una digestión eficiente sin sacrificar la integridad del ADN y, además, se obtuvieron cantidades suficientes para lograr un perfil genético sin ganancia (drop-in) ni pérdida (drop-out) de alelos (Liu, et al, 2012;Kitayama, et al, 2010).…”