Autologous transplantation with circulating hemopoietic stem cells

Zander, Axel R.; Cockerill, Kevin J.

doi:10.1002/jca.2920030402

Cited by 18 publications

(1 citation statement)

References 71 publications

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“…In this period progenitor cells can be collected in sufficient number for a graft performing daily leukapheresis with a fully automated blood cell separator [1,2]. The in vivo use of growth factors in combination with chemotherapy increases the release of hemopoietic progenitors into the peripheral blood, simplifying collection procedures [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator

Menichella¹,

Lai²,

Pierelli³

et al. 1997

J. Clin. Apheresis

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Section: Introductionmentioning

confidence: 99%

Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator

Menichella¹,

Lai²,

Pierelli³

et al. 1997

J. Clin. Apheresis

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Peripheral blood stem cell collection with reduced platelet loss to the patient/donor

Orlina

DeChristopher

Conant

et al. 1995

J of Clinical Apheresis

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Apheresis procedures that optimize peripheral blood stem cell (PBSC) harvesting also result in a significant loss of platelets to the patient/donor because of their similar densities. We compared the percent drop in platelet count and hemoglobin concentration in the patients before and after PBSC collection using two different collection chambers with the CS-3000. A modified plateletpheresis procedure was utilized. Seven patients underwent 38 PBSC collections during steady state hematopoiesis using the standard A-35 collection chamber. At the end of the procedure, a second low-speed centrifugation of the PBSC concentrate was performed in the manual mode, with siphoning out and return of the PRP to the patient through a transfer pack. For 14 patients who underwent 113 PBSC collections, a small volume collection chamber (SVCC) was substituted for the A-35 chamber and the second centrifugation step was omitted. These patients were also primed with 4 g/m2 of cyclophosphamide. The percent drop in platelet count in the patients after the collection procedures was significantly less in the SVCC group (20.4 +/- 9.1 vs. 36.0 +/- 12.3, P = 0.000), even after correction for the difference in the volume of blood processed between the two groups (3.2 +/- 1.4 vs. 3.9 +/- 1.3, P = 0.006). The percent drop in hemoglobin concentration was also less with the SVCC both before (5.4 +/- 3.8 vs. 11.7 +/- 3.0, P = 0.000) and after (0.8 +/- 0.6 vs. 1.3 +/- 0.3, P = 0.000) correction for the difference in the volume of blood processed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells

Law

Schwartz

Alsop

et al. 1989

The International Journal of Cell Cloning

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Purification of hematopoietic cells often includes an adherence step for the removal of monocytes. Recent studies have shown that some hematopoietic cells are adherent and that plastic adhesion might activate monocyte/macrophage for cytokine production. We investigated the possibility of using L-phenylalanine methyl ester (PME) as an alternative approach to monocyte removal, particularly for large-scale cell separations. Cell yield, extent of monocyte removal and cloning efficiency of colony-forming progenitor cells were compared to that produced by plastic adhesion. Starting with mononuclear cells (MNC) from adult peripheral blood, cell recovery after PME treatment was higher than that achieved with adherence and fewer monocytes were present. Cloning efficiency of the granulocyte-monocyte colony-forming progenitor cells (CFU-gm) was not affected by the PME treatment. PME treatment and plastic adhesion of low-density cord blood cells produced comparable cell recovery, monocyte depletion and cloning efficiencies for CFU-gm and burst forming units for erythroid progenitor cells (BFU-e). The PME procedure was optimized for the purification of hematopoietic cells from large quantities (less than 1 x 10(9)) of low density. T cell-depleted peripheral blood MNC containing 60% to 85% monocytes. Compared to two cycles of plastic adhesion, PME treatment permitted higher cell recovery (5.9% vs. 1.3%), lower monocyte contamination (4.5% vs. 10.7%) and substantial cost, time and labor savings without compromising CFU-gm growth.

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Autologous transplantation with circulating hemopoietic stem cells

Cited by 18 publications

References 71 publications

Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator

Evaluation of a new protocol for peripheral blood stem cell collection with the Fresenius AS 104 cell separator

Peripheral blood stem cell collection with reduced platelet loss to the patient/donor

Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells

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