Autologous Transplantation of Retinal Pigment Epithelium–Bruch’s Membrane Complex for Hemorrhagic Age-Related Macular Degeneration

Ma, Zhizhong; Han, Liang; Wang, Changguan; Dou, Hong-Liang; Hu, Yuntao; Feng, Xuefeng; Xu, Yimin; Wang, Zhiqiang; Yin, Zhinan; Liu, Yuling

doi:10.1167/iovs.08-2573

Cited by 44 publications

(37 citation statements)

References 33 publications

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“…In patients with advanced ARMD, where considerable photoreceptor damage has already occurred, preservation of these receptors is essential, making complete control of the manoeuvres in contact with the outer retina crucial. Similar results have now been described using a similar procedure by two other groups [29,30].…”

Section: Discussionsupporting

confidence: 83%

“…Recent studies have found significant incidences of this complication: revision surgery was required in five of 12 patients in a series by Joussen [32], and PVR was present in three of 21 patients in a series by Zhizhong [29]. In a long-term evaluation, Maaijwee noted an 8% incidence of retinal detachment (seven of 84 patients) associated, however, with a 90% rate of cellophane or macular pucker [21].…”

Section: Discussionmentioning

confidence: 99%

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Surgery for CNV and autologous choroidal RPE patch transplantation: exposing the submacular space

Cereda

Parolini

Bellesini

et al. 2009

Graefes Arch Clin Exp Ophthalmol

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Surgery for CNV and autologous choroidal RPE patch transplantation: exposing the submacular space

Cereda

Parolini

Bellesini

et al. 2009

Graefes Arch Clin Exp Ophthalmol

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“…Since RPE is being considered as one of the replacement tissues and since there are reports of varied RPE-secreted factors playing a role [55][56][57] in retinal differentiation and survival, we have explored a role that cell density might play in such rescue and differentiation. We report in this study that RPE-secreted factors enhance neuronal/photoreceptor differentiation and that (a) this differentiation is density dependent and (b) RPE might be secreting both photoreceptor differentiation and inhibition factors.…”

Section: Discussionmentioning

confidence: 99%

RPE-secreted factors: influence differentiation in human retinal cell line in dose- and density-dependent manner

Dutt

Douglas

Cao

2010

j ocul biol dis inform

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Retinal pigment epithelial (RPE) cells play an important role in normal functioning of retina and photoreceptors, and some retinal degenerations arise due to malfunctioning RPE. Retinal pigment epithelium transplantation is being explored as a strategy to rescue degenerating photoreceptors in diseases such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP). Additionally, RPE-secreted factors could rescue degenerating photoreceptors by prolonging survival or by their ability to differentiate and give rise to photoreceptors by transdifferentiation. In this study, we have explored what role cell density could play in differentiation induced in a human retinal progenitor cell line, in response to RPE-secreted growth factors. Retinal progenitors plated at low (1 × 10 4 cells/cm 2 ), medium (2-4×10 4 cells/cm 2 ), and high (1×10 5 cells/cm 2 ) cell density were exposed to various dilutions of RPE-conditioned medium (secreted factors) under conditions of defined medium culture. Progenitor cell differentiation was monitored phenotypically (morphological, biochemical analysis, and immunophenotyping, and western blot analysis were performed). Our data show that differentiation in response to RPE-secreted factors is modulated by cell density and dilutions of conditioned medium. We conclude that before embarking on RPE transplantation as a modality for treatment of RP and AMD, one will have to determine the role that cell density and inhibitory and stimulatory neurotrophins secreted by RPE could play in the efficacy of survival of transplants. We report that RPEconditioned medium enhances neuronal phenotype (photoreceptors, bipolars) at the lowest cell density in the absence of cell-cell contact. Eighty percent to 90% of progenitor cells differentiate into photoreceptors and bipolars at 50% concentration of conditioned medium, while exposure to 100% conditioned medium might increase multipolar neurons (ganglionic and amacrine phenotypes) to a small degree. However, no clear-cut pattern of differentiation in response to RPE-secreted factors is noted at higher cell densities.

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“…Impairment in the RPE and photoreceptor dysfunction causes AMD. Several investigators have reported that the transplantation of autologous RPE increased the vision of AMD patients (Ma et al 2009). However, this surgery has severe complications including the high risk of retinal hemorrhage.…”

Section: Rpe Transplantationmentioning

confidence: 99%

Induced pluripotent stem cells for retinal degenerative diseases: a new perspective on the challenges

Jin

Okamoto

Mandai

et al. 2009

J Genet

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Retinal degenerative diseases, including age-related macular degeneration and retinitis pigmentosa, are the prodominant causes of human blindness in the world; however, these diseases are difficult to treat. Currently, knowledge on the mechanisms of these diseases is still very limited and no radical drugs are available. Induced pluripotent stem (iPS) cells are an innovative technology that turns somatic cells into embryonic stem (ES)-like cells with pluripotent potential via the exogenous expression of several key genes. It can be used as an unlimited source for cell differentiation or tissue engineering, either of which is a promising therapy for human degenerative diseases. Induced pluripotent cells are both an unlimited source for retinal regeneration and an expectant tool for pharmaprojects and developmental or disease modelling. In this review, we try to summarize the advancement of iPS-based technologies and the potential utility for retinal degenerative diseases. We also discuss the challenges of using this technology in the retinology field.

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Autologous Transplantation of Retinal Pigment Epithelium–Bruch’s Membrane Complex for Hemorrhagic Age-Related Macular Degeneration

Abstract: The transplantation of the autologous RPE-Bruch's membrane complex can increase the visual acuity of patients with hemorrhagic AMD. The surviving transplanted graft with functional overlying retina was observed after surgery.

Cited by 44 publications

References 33 publications

Surgery for CNV and autologous choroidal RPE patch transplantation: exposing the submacular space

Surgery for CNV and autologous choroidal RPE patch transplantation: exposing the submacular space

RPE-secreted factors: influence differentiation in human retinal cell line in dose- and density-dependent manner

Induced pluripotent stem cells for retinal degenerative diseases: a new perspective on the challenges

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