Autologous transplantation of mesenchymal stromal cells tends to prevent progress of interstitial fibrosis in a rhesus Macaca mulatta monkey model of chronic kidney disease

Moghadasali, Reza; Hajinasrollah, Mostafa; Argani, Hassan; Nassiri, Seyed Mahdi; Najarasl, Mostafa; Sodeifi, Niloofar; Baharvand, Hossein; Aghdami, Nasser

doi:10.1016/j.jcyt.2015.06.006

Cited by 18 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, proofs of concepts were also established in preclinical porcine or simian models[ 70 - 72 ]. With this in mind, the use of USCs of urologic origin seems to be a judicious strategy based on their extra capacity of renal differentiation compared to MSCs.…”

Section: Uscs: Applicationsmentioning

confidence: 99%

Urine-derived stem/progenitor cells: A focus on their characterization and potential

Burdeyron

Giraud

Hauet

et al. 2020

WJSC

View full text Add to dashboard Cite

Cell therapy, i.e ., the use of cells to repair an affected tissue or organ, is at the forefront of regenerative and personalized medicine. Among the multiple cell types that have been used for this purpose [including adult stem cells such as mesenchymal stem cells or pluripotent stem cells], urine-derived stem cells (USCs) have aroused interest in the past years. USCs display classical features of mesenchymal stem cells such as differentiation capacity and immunomodulation. Importantly, they have the main advantage of being isolable from one sample of voided urine with a cheap and unpainful procedure, which is broadly applicable, whereas most adult stem cell types require invasive procedure. Moreover, USCs can be differentiated into renal cell types. This is of high interest for renal cell therapy-based regenerative approaches. This review will firstly describe the isolation and characterization of USCs. We will specifically present USC phenotype, which is not an object of consensus in the literature, as well as detail their differentiation capacity. In the second part of this review, we will present and discuss the main applications of USCs. These include use as a substrate to generate human induced pluripotent stem cells, but we will deeply focus on the use of USCs for cell therapy approaches with a detailed analysis depending on the targeted organ or system. Importantly, we will also focus on the applications that rely on the use of USC-derived products such as microvesicles including exosomes, which is a strategy being increasingly employed. In the last section, we will discuss the remaining barriers and challenges in the field of USC-based regenerative medicine.

show abstract

Section: Uscs: Applicationsmentioning

confidence: 99%

Urine-derived stem/progenitor cells: A focus on their characterization and potential

Burdeyron

Giraud

Hauet

et al. 2020

WJSC

View full text Add to dashboard Cite

show abstract

“…Franchi et al have reported that transplanted MSCs restored kidney function in a PKD model and improved damaged vasculature of these kidneys [13]. Previously we showed the promising efficacy of transplanted MSCs in experimental models of chronic kidney disease (CKD) and acute kidney injury (AKI) [14–16]. We had indicated that MSC-derived conditioned medium induced renal tubular cell regeneration after nephrotoxicity induction [14], then we showed the bone marrow MSC (BMMSC) lowered serum creatinine (SCr) and urea levels and accelerated regulatory T cells (Tregs) in a monkey AKI model [15].…”

Section: Introductionmentioning

confidence: 99%

“…We had indicated that MSC-derived conditioned medium induced renal tubular cell regeneration after nephrotoxicity induction [14], then we showed the bone marrow MSC (BMMSC) lowered serum creatinine (SCr) and urea levels and accelerated regulatory T cells (Tregs) in a monkey AKI model [15]. In a recent study, our results indicated that MSCs resulted in decreased CKD progression in a monkey model [16]. Carvalhosa et al reported that cells lining cysts in ADPKD consisted of polycystic CD133+ progenitor [17].…”

Section: Introductionmentioning

confidence: 99%

Safety and tolerability of autologous bone marrow mesenchymal stromal cells in ADPKD patients

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundAutosomal dominant polycystic kidney disease (ADPKD) is a genetic ciliopathy disease characterized by progressive formation and enlargement of cysts in multiple organs. The kidneys are particularly affected and patients may eventually develop end-stage renal disease (ESRD). We hypothesize that bone marrow mesenchymal stromal cells (BMMSCs) are renotropic and may improve kidney function via anti-apoptotic, anti-fibrotic, and anti-inflammatory effects. In this study, we aim to assess the safety and tolerability of a BMMSC infusion in ADPKD patients.MethodsWe performed a single-arm phase I clinical trial with a 12-month follow-up. This study enrolled six eligible ADPKD patients with an estimated glomerular filtration rate (eGFR) of 25–60 ml/min/1.73 m2. Patients received autologous cultured BMMSCs (2 × 106 cells/kg) through the cubital vein according to our infusion protocol. We investigated safety issues and kidney function during the follow-up visits, and compared the findings to baseline and 1 year prior to the intervention.ResultsThere were no patients lost to follow-up. We observed no cell-related adverse events (AE) and serious adverse events (SAE) after 12 months of follow-up. The mean eGFR value of 33.8 ± 5.3 ml/min/1.73 m2 1 year before cell infusion declined to 26.7 ± 3.1 ml/min/1.73 m2 at baseline (P = 0.03) and 25.8 ± 6.2 ml/min/1.73 m2 at the 12-month follow-up visit (P = 0.62). The mean serum creatinine (SCr) level of 2 ± 0.3 mg/dl 1 year before the infusion increased to 2.5 ± 0.4 mg/dl at baseline (P = 0.04) and 2.5 ± 0.6 mg/dl at the 12-month follow-up (P = 0.96). This indicated significant changes between the differences of these two periods (12 months before infusion to baseline, and 12 months after infusion to baseline) in SCr (P = 0.05), but not eGFR (P = 0.09).ConclusionsThis trial demonstrated the safety and tolerability of an intravenous transplantation of autologous BMMSCs. BMMSC efficacy in ADPKD patients should be investigated in a randomized placebo-controlled trial with a larger population, which we intend to perform.Trial registrationClinicalTrials.gov, NCT02166489. Registered on June 14, 2014.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0557-7) contains supplementary material, which is available to authorized users.

show abstract

“…In random cortical fields, the existence of increased mesangial matrix, diffused glomerulosclerosis, and mesangial cell proliferation was assessed between two experimental groups. Moreover, for each section, the percentage of cortex area affected by fibrosis that included interstitial cortical fibrosis, tubular loss with minimal fibrosis, and periglomerular fibrosis was determined as described previously (Moghadasali et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Systems and classical biology approaches unraveled role of olfactory receptors in progression of kidney fibrosis

Motahharynia

Moein

Kiyanpour

et al. 2020

Preprint

View full text Add to dashboard Cite

The olfactory receptors (ORs) which are mainly known as odor-sensors in the olfactory epithelium are distributed in several non-sensory tissues. Despite the specified role of some of these receptors in normal physiology of the kidney, little is known about their potential effect in renal disorders. In this study, using the holistic view of systems biology, it was determined that ORs are significantly changed during the progression of kidney fibrosis. For further validation, common differentially expressed ORs resulted from reanalysis of two time-course microarray datasets were selected for experimental evaluation in a validated murine model of unilateral ureteral obstruction. Transcriptional analysis demonstrated considerable changes in the expression pattern of Olfr1393, Olfr433, Olfr129, and Olfr161 during the progression of kidney fibrosis. In conclusion, our results highlight the impact of systems biology in determination of the underlying mechanisms of chronic diseases and indicate the importance of time-course approaches to unravel the patterns of gene expression. The novel ORs proposed in this study could be the subject of further functional investigations in the kidney.

show abstract

Autologous transplantation of mesenchymal stromal cells tends to prevent progress of interstitial fibrosis in a rhesus Macaca mulatta monkey model of chronic kidney disease

Cited by 18 publications

References 43 publications

Urine-derived stem/progenitor cells: A focus on their characterization and potential

Urine-derived stem/progenitor cells: A focus on their characterization and potential

Safety and tolerability of autologous bone marrow mesenchymal stromal cells in ADPKD patients

Systems and classical biology approaches unraveled role of olfactory receptors in progression of kidney fibrosis

Contact Info

Product

Resources

About