Autologous stimulation of human lymphocyte subpopulation.

Opelz, Gerhard; Kiuchi, Masahiro; Takasugi, Mitsuo; Terasaki, P I

doi:10.1084/jem.142.5.1327

Cited by 290 publications

(116 citation statements)

References 17 publications

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“…Several years ago, it was discovered that the mixing of autologous T cells with non-T cells resulted in a proliferative response of the T cells in response to stimulation by the non-T cells (1,2). This response was called the autologous mixed lymphocyte reaction (MLR).…”

Section: In Normal Individuals T Cells Are Stimulated To Proliferate mentioning

confidence: 99%

Studies of immune functions of patients with systemic lupus erythematosus

Sakane

Steinberg

Arnett

et al. 1979

Arthritis & Rheumatism

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In normal individuals T cells are stimulated to proliferate by autologous non-T cells; this is called the autologous mixed lymphocyte reaction (MLR). Previous studies demonstrated that such an autologous MLR was markedly impaired in patients with active systemic lupus erythematosus (SLE). To determine whether the defect resided in the responding cell or the stimulating cell, mixing experiments were performed using cells from identical twins. We identified two sets of identical twins discordant for SLE activity and correspondingly discordant in their degree of responsiveness in the autologous MLR. Reciprocal mixing experiments were performed in which T cells from one twin of each pair were mixed with non-T cells from the other twin of that pair. These studies indicated that patients with active SLE

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Section: In Normal Individuals T Cells Are Stimulated To Proliferate mentioning

confidence: 99%

Studies of immune functions of patients with systemic lupus erythematosus

Sakane

Steinberg

Arnett

et al. 1979

Arthritis & Rheumatism

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“…3, upper) (Fig. 4, lower left) (28,29 1 hr at room temperature with sufficient antisera to human leukocyte type I interferon to neutralize 100 units of antiviral activity. The antiviral activity in supernatants from both T cells and B cells with monocytes was reduced by at least 60% after incubation with antisera to type I interferon (30) indicating that the interferon was Type I rather than immune (Type II).…”

Section: Effect Of Recurrent Infection On Lymphocytementioning

confidence: 99%

Role of T lymphocytes in cellular immune responses during herpes simplex virus infection in humans.

Rasmussen¹,

Merigan²

1978

Proc. Natl. Acad. Sci. U.S.A.

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Lymphocyte blast transformation and interferon production in mononuclear cell cultures prepared on Ficoll-Hypaque gradients from individuals with herpes simplex virus-I infection were enhanced by a disease recurrence. Responses to both herpes simplex virus-2 and phytohemagglutinin were unaltered. Transformation to herpes simplex virus-i antigen was adversely affected by depleting either thymus-derived (T) lymphocytes or bone marrow-derived (B) lymphocytes to- In humans, cellular immunity to HSV has been studied by using different in vitro variables, most commonly, lymphocyte transformation. The significance of lymphocyte transformation as a correlate of susceptibility to recurrent HSV infection is not established. Defects in transformation to HSV antigen associated with acute disease have been detected by some investigators (6), but not by others (7-11). Defects in lymphokine production in vitro, for example, in migration inhibiting factor and interferon production have been reported during acute HSV infection (7,12). In cardiac transplant recipients, low responses of lymphocyte interferon production and transformation to HSV antigen are associated with both severe and prolonged HSV infection (13).It is important to measure responses of thymus-derived (T) lymphocytes to specific antigens in cellular immune reactions. Mitogens. Lyophilized phytohemagglutinin-P (PHA) (Difco, Detroit, MI) was reconstituted in 5 ml of phosphate buffered saline and diluted 1:30. Pokeweed mitogen (PWM) (Gibco, Grand Island, NY) was reconstituted in 10 ml of the buffered saline and diluted 1:60. Mononuclear Cell Cultures. The microculture assay for lymphocyte transformation and interferon production from cells separated on Ficoll/Hypaque (FH) gradients has been described (22). T lymphocytes were purified on nylon fiber columns (23). The eluate was centrifuged on a FH gradient. T lymphocytes so prepared gave at least 60% rosette formation with papain-treated sheep erythrocytes (24), and at least 95% were killed by anti-human T lymphocyte sera (25).Bone marrow-derived (B) lymphocyte-enriched populations were obtained by incubating 5 X 106 mononuclear cells per ml with 0.5% papain-treated sheep erythrocytes in 50% fetal calf serum for 15 min at room temperature. The mixture was centrifuged at 200 X g for 5 min and held for 1.5 hr at room temperature. After gently resuspending the cell pellet and centrifuging at 400 X g for 30 min on a FH gradient, the nonrosetted cells at the interface of the FH and medium had residual rosette-forming cells of less than 5% and, for most experiments, less than 1%. Of the nonrosetted cells, 10-70% had surface immunoglobulins and 10-40% were monocytes.For monocyte depletion, leukocytes from dextran-sedimented, heparinized blood were centrifuged at 200 X g, resuspended in 30 ml McCoy's 5A medium, and incubated for

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“…In the autologous mixed lymphocyte response, it has been reported that T cells proliferate in response to autologous non-T cells (26,27); an inhibitory effect of anti-Ia antibodies on this response has been reported from several laboratories (28)(29)(30). We recently reported (22) 2 that the sera of patients with active SLE contain anti-Ia antibodies of the IgG and IgM class.…”

Section: Antigenic Stimulation Of Normal Subjects With Purified Protementioning

confidence: 99%

Anti-Ia antibody in the sera of normal subjects after in vivo antigenic stimulation.

Okudaira¹,

Goodwin²,

Williams³

1982

The Journal of Experimental Medicine

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Although human Ia antigens were first recognized on B lymphocytes and macrophages (1, 2), recent studies have revealed that Ia antigens are also expressed on normal T cells (3-5), Langerhans cells (6), and some malignant cells (7,8). A central role for Ia antigens in the regulation of immune responsiveness is suggested by the observations that antibodies to Ia inhibit antigen presentation (9) and bind soluble T cell-derived i mmunoregulatory factors (10-13). Ia-positive cells have been reported to be necessary in autologous and allogeneic mixed lymphocyte reactions (auto-MLR and allo-MLR) 1 (14) and to play a significant role in suppressor cell functions (15,16).In some presumed autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE) (17) as well as in infectious mononucleosis (18) and graftvs.-host reaction (19), an increase in peripheral blood Ia-positive T cells has been recorded. Yu et al. (17) have reported that the percentage of peripheral blood Iapositive T cells in normals increases after tetanus immunization or skin testing with purified protein derivative (PPD). In the present report, we also found an elevation of Ia-positive T cells in three of six immunizations in vivo, whereas, in the remaining three individuals studied, a slight decrease was observed. A more consistent finding, however, was the production of IgM anti-Ia-reactive antibodies after in vivo immunization or antigenic stimulation. These naturally occurring anti-Ia antibodies might regulate the percentage of Ia-positive T cells in peripheral blood. Materials and MethodsPreparation of Lymphocytes. Peripheral blood mononuclear cells (PBMC) were separated from heparinized blood of normal donors by differential centrifugation over Ficoll-Hypaque. Purified T cells were obtained by incubation of PBMC with neurarninidase-treated sheep erythrocytes (SRBC) at 4°C for 1 h with subsequent Ficoll-Hypaque gradient centrifugation. The SRBC obtained in the rosetting fraction were lysed with ammonium chloride-Tris buffer. After washing, adherent cells were removed by incubation at 37°C for 45 min on glass petri dishes. Such T cell preparations were assayed for purity (>97%) using immunofluorescence and mouse

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Autologous stimulation of human lymphocyte subpopulation.

Cited by 290 publications

References 17 publications

Studies of immune functions of patients with systemic lupus erythematosus

Studies of immune functions of patients with systemic lupus erythematosus

Role of T lymphocytes in cellular immune responses during herpes simplex virus infection in humans.

Anti-Ia antibody in the sera of normal subjects after in vivo antigenic stimulation.

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