AUTOLOGOUS STEM CELL GRAFTING IN ACUTE MYELOID LEUKAEMIA: TECHNICAL APPROACH OF MARROW INCUBATION <i>IN VITRO</i> WITH PHARMACOLOGICAL AGENTS (PREREQUISITE FOR CLINICAL APPLICATIONS)

Hervé, P.; Tamayo, E.; Peters, A

doi:10.1111/j.1365-2141.1983.tb07320.x

Cited by 32 publications

(11 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ex vivo processing of autologous BM grafts is an important prerequisite for optimal cryopreservation to reduce volume and numbers of granulocytes and RBC of the native BM suspension [7, 8]. Chemical and immunological purging techniques require a homogeneous mononuclear cell (MNC) suspension prepared by ex vivo processing [9, 10]. Cryopreserved autologous BM grafts are thawed at 40 °C in a water bath prior to reinfusion into the patients [11].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Contamination of Autologous Bone Marrow: Reinfusion of& ;Culture-Positive Grafts Does Not Result in Clinical Sequelae during the Posttransplantation Course

et al. 1998

View full text Add to dashboard Cite

Objectives: Microbiological cultures and posttransplantation course were analyzed in order to investigate the incidence and clinical significance of bacterial contamination of autologous bone marrow (BM) grafts. Methods: Cultures were obtained from BM after collection, BM concentrate after processing, contaminated/cryopreserved BM at thawing, and from peripheral blood (PB) following autologous BM transplantation (ABMT). The posttransplantation course of patients grafted with culture-positive BM was recorded and compared with patients who underwent ABMT with noncontaminated BM grafts. Results: In 239 BM grafts processed, the incidence of microbiological contamination was 26.4% (n = 63). Fifty marrow grafts were contaminated by bacteria from the skin flora: coagulase-negative Staphylococcus (CNSC), Propionibacterium, and Corynebacterium species (79%). Thirty-eight patients underwent ABMT (day 0) with cryopreserved culture-positive BM, and 32 patients were evaluable for microbiological cultures at thawing: in 10 of 32 BM grafts CNSC was found prior to reinfusion. Following ABMT, PB cultures revealed CNSC in 5 of 38 patients between days +4 and +12. However, the late occurrence of positive PB cultures after BM reinfusion made a relationship between BM CNSC and PB CNSC unlikely. In 33 of 38 patients, no graft-contaminating bacteria were detected in PB. Comparison of the posttransplantation course of patients who received contaminated BM with that of patients grafted with noncontaminated BM showed no significant differences concerning time to engraftment, febrile days, and days on antibiotics. Conclusion: (1) Collection and/or ex vivo processing can result in microbiological contamination of BM grafts predominantly with bacteria from the skin flora, and (2) only CNSC can be cultured at thawing from previously contaminated/cryopreserved BM. Since patients undergoing ABMT usually receive oral antibiotics from beginning of the conditioning regimen which are active against CNSC, no further administration of antibiotics is recommended for the reinfusion of bacterially contaminated BM grafts.

show abstract

Section: Introductionmentioning

confidence: 99%

Bacterial Contamination of Autologous Bone Marrow: Reinfusion of& ;Culture-Positive Grafts Does Not Result in Clinical Sequelae during the Posttransplantation Course

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Although this hypothesis holds good for certain exponentiallygrowing experimental tumours (such as the L1210 leukaemia), it is less satisfactory for most experimental and clinical tumours which seem to more closely follow Gompertzian growth kinetics (Norton & Simon, 1977). Thus there have been reports that, for some agents, cytotoxicity in vitro is profoundly affected by the ratio of cell concentration to drug dose, both in established cell lines (Chambers et al, 1984;Arkin et al, 1984) and in primary cultures (K6rbling et al, 1982;Herve et al, 1983). For this reason we have also examined the relationship between cell concentration and cell kill in the DiSC assay for five different drugs, using peripheral blood lymphocytes from CLL patients.…”

mentioning

confidence: 99%

The influence of sample source and cell concentration on the in vitro chemosensitivity of haematological tumours

Bird¹,

Forskitt²,

Gilby³

et al. 1986

Br J Cancer

View full text Add to dashboard Cite

Summary The Differential Staining Cytotoxicity (DiSC) assay has been used to study the effects of sample source and cell concentration on the in vitro chemosensitivity of haematological malignancies.The chemosensitivity of blood and bone marrow samples was significantly associated (P<0.001) in 12 cases where both were tested simultaneously. In 8 of the cases, where the in vitro result could be compared with clinical response, the in vitro and in vivo chemosensitivity was in agreement in 7, for both blood and bone marrow samples.The in vitro chemosensitivity of chronic lymphocytic leukaemia blood lymphocytes was dependent on the cell concentration for 4 out of 5 drugs tested. A five fold reduction in cell number resulted in a significantly greater cell kill with 4-hydroperoxycyclophosphamide, a greater cell kill (not significant) with chlorambucil and adriamycin, and a significantly lower cell kill with prednisolone. The cell concentration did not affect vincristine cytotoxicity.These results suggest that sample source is not an important consideration for the in vitro chemosensitivity of leukaemias, but that the cell concentration tested should not be varied from assay to assay if the results are to be used for comparative purposes.

show abstract

“…20 ± 7% of the red blood cells and 99 ± 29% of the CFU-GM were recovered in the buffy coat fraction (table II). The loss of CFU-GM in the pellet fraction was only 4% [2], Buffy coat collection took about 1.5 h.…”

Section: Two-step Separation Proceduresmentioning

confidence: 94%

“…Their results are comparable with our data obtained by floatation centrifugation in the Haemonetics with respect to red cell elimination (99%) and recovery of mononuclear cells (18% with the Haemonetics, 25% with the IBM). Clonogenic cell recovery seems to be better with the IBM, 109% compared to 67% in the Haemonetics, but with a considerable range, 53-170%, while data on the loss of clonogenic cells in the pellet fraction were not [1,2] and one with an acute lymphoblastic leukemia [3] received bone marrow separated by floatation only (method A). Three other patients with a solid tumor [4][5][6] were treated with low-density cells obtained after the two-step procedure (method B).…”

Section: Repopulation Capacitymentioning

confidence: 97%

“…Most depletion techniques, e. g. the use of mono clonal antibodies [1], cytotoxic drugs [2] or physical sep aration [3], require a primary enrichment of bone marrow aspirates. Isopycnic density separation for low-density cells (d< 1.070 g/ml) removes almost all erythrocytes and about 80% of the nucleated cells from the aspirates with out substantial loss of clonogenic cells [4], Density sepa ration based on isopycnic floatation centrifugation showed a higher recovery of clonogenic cells in the lowdensity cell fraction compared with isopycnic density sedimentation centrifugation [4], However, to process about 1 liter of bone marrow aspirate by isopycnic floa…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enrichment of Human Bone Marrow Aspirates for Low-Density Mononuclear Cells Using a Haemonetics Discontinuous Blood Cell Separator

Raijmakers¹,

Witte²,

Koekman³

et al. 1986

Vox Sanguinis

View full text Add to dashboard Cite

Isopycnic density floatation centrifugation has been proven to be a suitable technique to enrich bone marrow aspirates for clonogenic cells on a small scale. We have tested a Haemonetics semicontinuous blood cell separator in order to process large volumes of bone marrow with minimal bone marrow manipulation. The efficacy of isopycnic density floatation was tested in a one and a two-step procedure. Both procedures showed a recovery of about 20% of the nucleated cells and 1-2% of the erythrocytes. The enrichment of clonogenic cells in the one-step procedure appeared superior to the two-step enrichment, first separating buffy coat cells. The recovery of clonogenic cells was 70 and 50%, respectively. Repopulation capacity of the low-density cell fraction containing the clonogenic cells was excellent after autologous reinfusion (6 cases) and allogeneic bone marrow transplantation (3 cases). Fast enrichment of large volumes of bone marrow aspirates with low-density cells containing the clonogenic cells by isopycnic density floatation centrifugation can be done safely using a Haemonetics blood cell separator.

show abstract

AUTOLOGOUS STEM CELL GRAFTING IN ACUTE MYELOID LEUKAEMIA: TECHNICAL APPROACH OF MARROW INCUBATION IN VITRO WITH PHARMACOLOGICAL AGENTS (PREREQUISITE FOR CLINICAL APPLICATIONS)

Cited by 32 publications

References 3 publications

Bacterial Contamination of Autologous Bone Marrow: Reinfusion of& ;Culture-Positive Grafts Does Not Result in Clinical Sequelae during the Posttransplantation Course

Bacterial Contamination of Autologous Bone Marrow: Reinfusion of& ;Culture-Positive Grafts Does Not Result in Clinical Sequelae during the Posttransplantation Course

The influence of sample source and cell concentration on the in vitro chemosensitivity of haematological tumours

Enrichment of Human Bone Marrow Aspirates for Low-Density Mononuclear Cells Using a Haemonetics Discontinuous Blood Cell Separator

Contact Info

Product

Resources

About