Autologous porcine heart cell transplantation improved heart function after a myocardial infarction

Li, Ren‐Ke; Weisel, Richard D.; Mickle, Donald A.G.; Jia, Zhiqiang; Kim, Eung-Joong; Sakai, Tetsuro; Tomita, Shinji; Schwartz, Leonard; Iwanochko, Mark; Husain, Mansoor; Cusimano, Robert J.; Burns, Robert J.; Yau, Terrence M.

doi:10.1016/s0022-5223(00)70218-2

Cited by 146 publications

(80 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This cell loss may be partially offset by natural regeneration of cardiac myocytes in adult hearts (6). Cell transplantation to treat heart disease has been studied in various animal models (9,14,16,19,25). Cell types used include ventricular cardiomyocytes, skeletal myoblasts, smooth muscle cells, fetal and embryonic cardiomyocytes, bone marrow stromal, hematopoietic stem cells, and mouse embryonic stem (ES) cells (9,14,16,19,25).…”

mentioning

confidence: 99%

Factors released from embryonic stem cells inhibit apoptosis of H9c2 cells

Singla

McDonald²

2007

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

Our recent study (Singla DK, Hacker TA, Ma L, Douglas PS, Sullivan R, Lyons GE, Kamp TJ, J Mol Cell Cardiol 40: 195-200, 2006) suggests that transplanted embryonic stem (ES) cells subsequent to myocardial infarction differentiate into the major cell types in the heart and improve cardiac function. However, the extent of regeneration is relatively meager compared with the observed functional improvement. The mechanisms underlying their improved function are completely unknown. In this report, we provide evidence using a cell culture model system for novel mechanisms that involve the release of cytoprotective, antiapoptotic factor(s) from ES cells and inhibit H 2O2-induced apoptosis in the rat cardiomyocyte-derived cell line H9c2. Conditioned medium (CM) from growing mouse ES cells treated with and without H2O2 was generated. Apoptosis was induced after exposure to H2O2 in H9c2 cells for 2 h followed by replacement with fresh cell culture or ES cell-CM. After 24 h, H9c2 cells treated with both ES cell-CMs demonstrated significantly decreased apoptosis, as determined by terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling staining, apoptotic ELISA, caspase-3 activity, and DNA ladder. Next, using Luminex technology, we examined the presence of antiapoptotic proteins cystatin c, osteopontin, and clusterin and anti-fibrotic, tissue inhibitor of metalloproteinase-1 (TIMP-1) in both ES cell-CMs. The levels of released factors were 2-to 170-fold higher than those in H9c2 cell-CM. Antiapoptotic effects of ES cell-CM were significantly inhibited with TIMP-1 antibody, suggesting that TIMP-1 is an important factor to inhibit apoptosis. Furthermore, we used CM from an TIMP-1-overexpressing cell line and demonstrated that H2O2-induced apoptosis in the H9c2 cells was significantly inhibited. These observations demonstrate that factors released from ES cells contain antiapoptotic factors and that the effects are mediated by TIMP-1. Moreover, these findings suggest that released factors might be useful for therapeutic applications in ischemic heart disease as well as for many other diseases. hydrogen peroxide; tissue inhibitor of metalloproteinase-1

show abstract

mentioning

confidence: 99%

Factors released from embryonic stem cells inhibit apoptosis of H9c2 cells

Singla

McDonald²

2007

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…Studies in animal models have shown that transplantation of fetal stem cell, ES cells, or pluripotent stem cell derivatives can successfully treat many chronic diseases, such as Parkinson's disease, diabetes, traumatic spinal cord injury, Purkinje cell degeneration, Duchenne's muscular dystrophy, liver or heart failure, and osteogenesis imperfecta (Zhang et al, 1996;Horwitz et al, 1999;McDonald et al, 1999;Kobayashi et al, 2000;Li et al, 2000;Soria et al, 2000;Kim et al, 2002). Almost every day there are reports in the media of new stem cell therapies.…”

Section: Resultsmentioning

confidence: 99%

Embryonic Stem Cells: from Blastocyst to in vitro Differentiation

Manganelli¹,

Fico²,

Filosa³

2011

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applicatio

View full text Add to dashboard Cite

“…Fetal and neonatal cardiomyocytes have been tested for this purpose [62,63]. However, the implanted cardiomyocytes were unable to integrate with the native myocardium and caused arrhythmias [64]. In addition, the source for human fetal or neonatal cardiomyocytes is a concern.…”

Section: Cells Available For Deliverymentioning

confidence: 99%

Hydrogels for Cardiac Tissue Engineering

2011

View full text Add to dashboard Cite

Cardiac tissue regeneration is an integrated process involving both cells and supporting matrix. Cardiomyocytes and stem cells are utilized to regenerate cardiac tissue. Hydrogels, because of their tissue-like properties, have been used as supporting matrices to deliver cells into infarcted cardiac muscle. Bioactive and biocompatible hydrogels mimicking biochemical and biomechanical microenvironments in native tissue are needed for successful cardiac tissue regeneration. These hydrogels not only retain cells in the infarcted area, but also provide support for restoring myocardial wall stress and cell survival and functioning. Many hydrogels, including natural polymer hydrogels, synthetic polymer hydrogels, and natural/synthetic hybrid hydrogels are employed for cardiac tissue engineering. In this review, types of hydrogels used for cardiac tissue engineering are briefly introduced. Their advantages and disadvantages are discussed. Furthermore, strategies for cardiac regeneration using hydrogels are reviewed.

show abstract

Autologous porcine heart cell transplantation improved heart function after a myocardial infarction

Abstract: Autologous porcine heart cell transplantation improved regional perfusion and global ventricular function after a myocardial infarction.

Cited by 146 publications

References 14 publications

Factors released from embryonic stem cells inhibit apoptosis of H9c2 cells

Factors released from embryonic stem cells inhibit apoptosis of H9c2 cells

Embryonic Stem Cells: from Blastocyst to in vitro Differentiation

Hydrogels for Cardiac Tissue Engineering

Contact Info

Product

Resources

About