Autologous platelet-rich plasma for wound and osseous healing: A review of the literature and commercially available products

Abstract

The application of autologous platelets that have been sequestered, concentrated, and mixed with thrombin to create growth factor-concentrated, autologous platelet-rich plasma for application to soft tissue wounds and for osseous healing has been a subject of great interest for much of the past 2 decades. Autologous platelet-rich plasma, which consists of both quantitative and qualitative components, has the greatest potency or ability to produce the desired effect. Manufacturers prepare autologous platelet-rich plasma with the ultimate goal of maximizing its benefits while minimizing potential risks. Unfortunately, the manufacturing processes for autologous platelet-rich plasma are highly variable, and the types of proprietary systems available on the market for soft tissue and osseous applications are numerous. The authors provide here an in-depth review of commercially available systems for delivery of autologous platelet-rich plasma that emphasizes the subtle yet important differences among systems. In addition, a detailed review of the literature regarding the use of autologous platelet-rich plasma in soft tissue and osseous healing is provided. Although findings are not yet conclusive, autologous platelet-rich plasma has been shown to be safe, reproducible, and effective in mimicking the natural processes of soft tissue wound and osseous healing.

Platelet rich plasma and amnion-derived fluid as clinical options for regenerative medicine applications

“…Furthermore, the production of a PRP product depends significantly on the standard operating procedures that are used. Many clinics have unique or proprietary protocols that must be followed to successfully create a therapeutically-active PRP [23]. This introduces additional variables to consider in the clinic when different technicians perform the blood draw, subsequent centrifugations, and PRP harvest.…”

Plasma rico em plaquetas: mecanismo de ação, produção e indicações de uso - Revisão de literatura

“…Os FC´s envolvidos na recuperação tecidual e que comprovadamente são produzidos por plaquetas são o fator de crescimento derivado das plaquetas (PDGF), fator de crescimento transformador beta (TGFβ), fator de crescimento vascular endotelial (VEGF), fator de crescimento epitelial (EGF) (Marx, 2004;Roukis et al, 2006), fator de crescimento fibroblástico (FGF) e fator de crescimento semelhante a insulina (IGF) (Bolta, 2007;Moreira et al, 2008).…”

“…Com relação ao processo de centrifugação, etapa essencial para a fabricação do PRP ( Levando em consideração a variação das densidades, após a centrifugação de uma amostra de sangue, as hemácias, que tem maior peso, tendem a se depositar no fundo do tubo, enquanto que os leucócitos se localizam em uma zona intermediária, e o plasma fica no alto (Roukis et al, 2006). A junção das plaquetas e leucócitos corresponde a uma camada esbranquiçada chamada de zona de névoa (Jain, 1993) (Figura 1).…”

“…São fatores agonistas indicados para ativar o PRP, a trombina bovina (Roukis et al, 2006;Pagliosa e Alves, 2007), tromboplastina ou a trombina autógena (Barbosa et al, 2008). Já foi citado também o uso de outras substâncias, tais como o batroxobin (produto isolado do veneno de serpentes) (Bolta, 2007).…”

Platelet rich plasma: traitement des tendinopathies chroniques? Revue de la littérature

“…Certains travaux, essentiellement in vitro, soulignent les propriétés réparatrices des plaquettes qui, effectivement, présentent la capacité d'accélérer la cicatrisation de différents tissus : os, muscles et tendons [8][9][10][11][12][13][14]. Le plasma enrichi en plaquettes (plateletrich plasma ou PRP) a déjà été utilisé en chirurgie maxillofaciale, en stomatologie ou en chirurgie orthopédique [9,10].…”