Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review

Troha, Kaja; Vozel, Domen; Arko, Matevž; Zavec, Apolonija Bedina; Dolinar, Drago; Hočevar, Matej; Jan, Zala; Kisovec, Matic; Kocjančič, Boštjan; Pađen, Ljubiša; Pajnič, Manca; Penič, Samo; Romolo, Anna; Repar, Neža; Spasovski, Vesna; Steiner, Nejc; Šuštar, Vid; Iglič, Aleš; Drobne, Damjana; Kogej, Ksenija; Battelino, Saba; Kralj‐Iglič, Veronika

doi:10.3390/ijms24043420

Cited by 8 publications

(2 citation statements)

References 267 publications

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“…Therefore, this PL gel is likely to be used as a short-term treatment in the future. The application of platelet lysate in gel form is better than liquid form because gel prevents the platelet content from dispersing from the desired site to other parts [ 25 ]. Thus, the gel can restrict the movement of molecular content and increase the effectiveness of the treatment.…”

Section: Resultsmentioning

confidence: 99%

Effects of Platelet Lysate Gels Derived from Different Blood Sources on Oral Mucosal Wound Healing: An In Vitro Study

Azhar

Budin

et al. 2023

Gels

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The rapid healing of oral ulcers is important to prevent secondary infection, especially for chronic oral ulcers. Platelet lysate (PL) is rich in growth factors for cell growth and promotes tissue regeneration. Hence, this study was performed to compare the effects of PL originating from umbilical cord blood (CB) and peripheral blood (PB) on oral mucosal wound healing. The PLs were molded into gel form in the culture insert with the addition of calcium chloride and conditioned medium for sustained release of growth factors. The CB-PL and PB-PL gels were found to degrade slowly in culture and their degradation percentages by weight were 5.28 ± 0.72% and 9.55 ± 1.82% respectively. The results from the scratch assay and Alamar blue assay showed that the CB-PL and PB-PL gels increased the proliferation (148 ± 3% and 149 ± 3%) and wound closure (94.17 ± 1.77% and 92.75 ± 1.80%) of oral mucosal fibroblasts compared to the control with no statistical differences between the two gels, respectively. Quantitative RT-PCR showed that mRNA expressions of collagen-I, collagen-III, fibronectin, and elastin genes in cells treated with CB-PL (11-, 7-, 2-, and 7-fold) and PB-PL (17-, 14-, 3-, and 7-fold) decreased compared with the control, respectively. The concentration of platelet-derived growth factor of PB-PL gel (1303.10 ± 343.96 pg/mL) showed a higher trend than CB-PL gel did (905.48 ± 69.65 pg/mL) from ELISA measurement. In summary, CB-PL gel is as effective as PB-PL gel in supporting oral mucosal wound healing, making it a potential new source of PL for regenerative treatment.

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Section: Resultsmentioning

confidence: 99%

Effects of Platelet Lysate Gels Derived from Different Blood Sources on Oral Mucosal Wound Healing: An In Vitro Study

Azhar

Budin

et al. 2023

Gels

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“…Owing to the repetitive nature of the commonly employed treatment strategies for chronic tissue injuries, there has been an enormous socio-economic burden worldwide. The advent of the various forms of PRP and its derivatives, which have prolonged shelf life and the provision of their storage as lyophilized products without loss of efficacy, allows for the creation of a readily available source of therapeutics with multifold applications [218]. The field of patient care and treatment options has been revolutionized by groundbreaking technologies and innovations such as 3D printing [219].…”

Section: Challenges and Future Scopementioning

confidence: 99%

Recent advances in platelet-rich plasma and its derivatives: therapeutic agents for tissue engineering and regenerative medicine

Shome,

Kodieswaran,

Dadheech

et al. 2024

Prog. Biomed. Eng.

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Platelet rich plasma (PRP) is a suspension of bioactive factors and chemokine enriched plasma. Platelets are a distinctive source of membrane bound and soluble proteins that are released upon their activation. The higher count of platelets renders PRP with an array of tissue regenerative abilities. PRP can be employed in the form of platelet containing plasma, platelet lysate plasma, or in the form of a pre-gelled fibrin matrix. PRP has been an essential alternative source of growth factors in the healing and regeneration of various tissues, such as musculoskeletal, cardiovascular, and dermal tissue, with additional applications in other tissues, such as hepatic and neural. A wide range of preparative and isolation strategies have been developed for various forms of PRP at laboratory and commercial scales. Concomitantly, PRP has found its applicability as an active component in several tissue regenerative approaches, including 3D printed/bioprinted constructs, injectable hydrogels, and crosslinked scaffolds. This review focuses on the various forms of PRP and their preparation methods, the latest tissue engineering applications of PRP, and the various tissue-specific clinical trials and findings conducted using PRP. We have further discussed the optimizations required in the methods of preparation, delivery, and long-term storage of PRP. Therefore, this review seeks to benefit the scope of research on PRP-based therapeutic agents in tissue engineering by providing comprehensive insights into the widespread application. We believe PRP could be instrumental in future patient-specific tissue engineering applications in both pre-clinical and clinical settings.

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Platelet-rich plasma and other blood derivatives—new research on the effect of blood derivatives and clinical results in osteoarthritis of the knee

Otahal,

Tischer,

De Luna

et al. 2023

Knie J.

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ZusammenfassungDie Anwendung von Blutprodukten in der orthopädischen Praxis erlangte durch die Etablierung ambulanter Verfahren mittels Zentrifugation größeres Aufsehen, da dadurch Point-of-care-Behandlungen mit autologen Blutbestandteilen leicht möglich wurden. Besonders Thrombozytenkonzentrate wie plättchenreiches Plasma (PRP) sind für Anwendungen am Bewegungsapparat in den Fokus gerückt. Die mittlerweile große Anzahl an Herstellungsverfahren und Anwendungstechnologien und daraus resultierende unterschiedliche Zusammensetzungen der Blutderivate machen es schwierig, generelle Wirkprinzipien zu beschreiben und Indikationen abzuleiten. Studien zu Wirkmechanismen von PRP und anderen Blutderivaten konzentrierten sich daher bisher auf die enthaltenen Wachstumsfaktoren. Neue Erkenntnisse zeigen, dass zusätzlich nachhaltige Veränderungen durch extrazelluläre Vesikel – bisher wenig beachtete Komponenten von Blut und Blutprodukten – erreicht werden und darin enthaltene Signalstoffe wie microRNAs die Zellphysiologie beeinflussen können. Durch Standardisierung von Herstellungs- und Applikationsverfahren könnte eine bessere Darstellung von Wirksamkeiten im klinischen Einsatz abgesichert werden. Die meisten aktuell verfügbaren klinischen Arbeiten zeigen positive Wirkungen in der Behandlung der Gonarthrose, sowohl gegenüber Placebo als auch anderen Injektionsmethoden, wobei der klinische Einsatz aktuell aber noch widersprüchlich diskutiert wird.

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Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review

Cited by 8 publications

References 267 publications

Effects of Platelet Lysate Gels Derived from Different Blood Sources on Oral Mucosal Wound Healing: An In Vitro Study

Effects of Platelet Lysate Gels Derived from Different Blood Sources on Oral Mucosal Wound Healing: An In Vitro Study

Recent advances in platelet-rich plasma and its derivatives: therapeutic agents for tissue engineering and regenerative medicine

Platelet-rich plasma and other blood derivatives—new research on the effect of blood derivatives and clinical results in osteoarthritis of the knee

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