Autologous Matrix-Induced Chondrogenesis: A Systematic Review of the Clinical Evidence

Gao, Liang; Orth, Patrick; Cucchiarini, Magali

doi:10.1177/0363546517740575

Cited by 91 publications

(68 citation statements)

References 63 publications

(125 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A possible translational clinical scenario where such scaffolds could be implanted into the subchondral bone would be as an adjunct to marrow stimulation techniques for cartilage repair (6). When implanted into the subchondral penetrations, such porous TGF-b1-releasing scaffolds could induce chondrogenesis of migrating MSCs into the cartilage defect.…”

Section: Discussionmentioning

confidence: 99%

“…Cell condensation occurs in the cartilage defect, precursor mesenchymal cells then divide and differentiate into chondrogenic and osteogenic lineages, maturate, and deposit extracellular matrix. This process is tightly controlled by growth and transcription factors, paracrine interactions with articular cartilage that surrounds the defect, and other environmental factors (1,6). As a result, a new cartilaginous repair tissue that is rich in type II collagen and proteoglycans fills the defect, whereas endochondral ossification restores the subchondral bone.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Sustained spatiotemporal release of TGF‐β1 confers enhanced very early chondrogenic differentiation during osteochondral repair in specific topographic patterns

et al. 2018

Self Cite

View full text Add to dashboard Cite

The continuous presence of TGF-β is critically important to induce effective chondrogenesis. To investigate chondrogenesis in a cartilage defect, we tested the hypothesis that the implantation of TGF-β1-releasing scaffolds improves very early cartilage repair in vivo. Spatiotemporal controlled release of TGF-β1 was achieved from multiblock scaffolds that were implanted in osteochondral defects in the medial femoral condyles of adult minipigs. We observed a sustained presence of TGF-β1 at 4 wk in vivo, which significantly promoted structural aspects of early overall cartilage repair, especially cellularity, cellular morphology, and safranin O staining intensity. Furthermore, early aggrecan and type II collagen production were both increased in specific topographic patterns in cartilaginous repair tissue. Sustained release of TGF-β1 also increased cell numbers and proliferation, staining intensities for the stem cell surface marker, CD105, and number of stromal cell-derived factor-1 (SDF-1) -positive cells within cartilaginous repair tissue. These data identify a mechanism by which TGF-β1 modulates early chondrogenesis by primarily increasing the number of progenitor cells arising from the subchondral bone marrow compartment via the SDF-1/chemokine (CXC motif) receptor 4 pathway, their proliferation, differentiation, and extracellular matrix deposition in specific topographic patterns, highlighting the pivotal role played by TGF-β1 during this crucial phase.-Asen, A.-K., Goebel, L., Rey-Rico, A., Sohier, J., Zurakowski, D., Cucchiarini, M., Madry, H. Sustained spatiotemporal release of TGF-β1 confers enhanced very early chondrogenic differentiation during osteochondral repair in specific topographic patterns.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Sustained spatiotemporal release of TGF‐β1 confers enhanced very early chondrogenic differentiation during osteochondral repair in specific topographic patterns

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Since more recent ACI versions are based on collagen carriers (27), the chondrocytes were cultured on collagen type II-coated membranes in a 24-wells transwell system as described previously (28). Passage two chondrocytes were seeded on inserts (PICM01250, Millipore) with a hydrophilic poly-tetrafluoroethylene (PTFE) membrane at 1.6×10 6 cells/cm 2 in DMEM with 2% w/v ITSx (51500, Invitrogen), 2% w/v ascorbic acid (A8960, Sigma-Aldrich), 2% w/v human serum albumin (HS-440, Seracare Life Sciences), 100 units/mL penicillin, 100 μg/mL streptomycin, and 10 ng/mL TGF-β 2 (302-B2, R&D Systems).…”

Section: Methodsmentioning

confidence: 99%

PTH decreasesin vitrohuman cartilage regeneration without affecting hypertrophic differentiation

Rutgers

Bach

Vonk

et al. 2019

Preprint

View full text Add to dashboard Cite

Regenerated cartilage formed after Autologous Chondrocyte Implantation may be of suboptimal quality due to postulated hypertrophic changes. Parathyroid hormone-related peptide, containing the parathyroid hormone sequence (PTHrP 1-34), enhances cartilage growth during development and inhibits hypertrophic differentiation of mesenchymal stromal cells (MSCs) and growth plate chondrocytes. This study aims to determine whether human articular chondrocytes respond correspondingly. Healthy human articular cartilage-derived chondrocytes (n=6 donors) were cultured on type II collagen-coated transwells with/without 0.1 or 1.0 μM PTH from day 0, 9, or 21 until the end of culture (day 28). Extracellular matrix production, (pre)hypertrophy and PTH signaling were assessed by RT-qPCR and/or immunohistochemistry for collagen type I, II, X, RUNX2, MMP13, PTHR1 and IHH and by determining glycosaminoglycan production and DNA content. The Bern score assessed cartilage quality by histology. Regardless of the concentration and initiation of supplementation, PTH treatment significantly decreased DNA and glycosaminoglycan content and reduced the Bern score compared with controls. Type I collagen deposition was increased, whereas PTHR1 expression and type II collagen deposition were decreased by PTH supplementation. Expression of the (pre)hypertrophic markers MMP13, RUNX2, IHH and type X collagen were not affected by PTH. In conclusion, PTH supplementation to healthy human articular chondrocytes did not affect hypertrophic differentiation, but negatively influenced cartilage quality, the tissues’ extracellular matrix and cell content. Although PTH may be an effective inhibitor of hypertrophic differentiation in MSC-based cartilage repair, care may be warranted in applying accessory PTH treatment due to its effects on articular chondrocytes.

show abstract

“…18,44 Autologous Matrix-induced Chondrogenesis AMIC was first introduced in 2010 and describes a combination of bone marrow stimulation, for example, MFx with the placement of a acellular collagen matrix onto the cartilage defect. [61][62][63] The first steps of AMIC resemble those of the MFx Fig. 3 Sagittal proton-density-weighted fat-suppressed MR images (a) 6 months, (b) 1 year, and (c) 2 years following autologous osteochondral transplantation (OAT) in a 45-year-old male patient (same patient as in Fig.…”

Section: Osteochondral Allograft Transplantationmentioning

confidence: 99%

“…Additionally, the type I/III collagen matrix is trimmed to the size of the cartilage defect and fixated using either sutures or fibrin glue. 62 AMIC is generally used to treat articular cartilage defects > 2 cm. 2,63 In many clinical studies it could be shown that AMIC leads to a good clinical improvement of symptoms.…”

Section: Osteochondral Allograft Transplantationmentioning

confidence: 99%

Imaging of the Knee Following Repair of Focal Articular Cartilage Lesions

Wuennemann

Rehnitz

Weber

2018

Semin Musculoskelet Radiol

View full text Add to dashboard Cite

Focal chondral or osteochondral lesions of the knee are common lesions involving either the cartilage layers or the cartilage layers and the subchondral bone. Despite their heterogeneous clinical presentation, they are important risk factors for the premature development of osteoarthritis. Therefore, early detection of osteochondral lesions and focal cartilage defects is crucial. In symptomatic (osteo-)chondral lesions, numerous therapeutic strategies, ranging from conservative treatment to surgical procedures such as marrow stimulation, osteochondral autograft transplantation, or autologous chondrocyte implantation are available. Musculoskeletal radiologists should be familiar with these surgical procedures, the evaluation of the postoperative findings as well as the possible complications when interpreting postoperative imaging studies. This review article describes the different surgical approaches to focal osteochondral lesions of the knee with emphasis on postoperative imaging findings and the pitfalls possibly encountered by the radiologist.

show abstract

Autologous Matrix-Induced Chondrogenesis: A Systematic Review of the Clinical Evidence

Cited by 91 publications

References 63 publications

Sustained spatiotemporal release of TGF‐β1 confers enhanced very early chondrogenic differentiation during osteochondral repair in specific topographic patterns

Sustained spatiotemporal release of TGF‐β1 confers enhanced very early chondrogenic differentiation during osteochondral repair in specific topographic patterns

PTH decreasesin vitrohuman cartilage regeneration without affecting hypertrophic differentiation

Imaging of the Knee Following Repair of Focal Articular Cartilage Lesions

Contact Info

Product

Resources

About