Autologous Fibroblast Culture in the Repair of Aging Skin

Eça, Lilian Piñero; Pinto, Débora Galdino; Pinho, Ariene Murari Soares de; Mazzetti, Marcelo Paulo Vaccari; Odo, Marina Emiko Yagima

doi:10.1111/j.1524-4725.2011.02192.x

Cited by 23 publications

(7 citation statements)

References 17 publications

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“…In clinical aesthetic medicine, epidermal or intradermal injection of hyaluronic acid is performed to obtain glossy and healthy skin (microinjections of hyaluronic acid, vitamins, minerals, and amino acids into the superficial layer of the skin) [15]. Other techniques, such as implanting activated fibroblasts in the skin, are also known to revive the skin to be glossy and healthy (intradermal injection of cultivated skin fibroblasts into wrinkles) [16-18]. However, these therapies are associated with a high cost and may provoke adverse events, including misplacement, allergy, nodules, necrosis, abscesses, and rejection.…”

Section: Discussionmentioning

confidence: 99%

(+)-Catechin protects dermal fibroblasts against oxidative stress-induced apoptosis

Tanigawa

Kanazawa

Ichibori

et al. 2014

BMC Complement Altern Med

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BackgroundOxidative stress has been suggested as a mechanism underlying skin aging, as it triggers apoptosis in various cell types, including fibroblasts, which play important roles in the preservation of healthy, youthful skin. Catechins, which are antioxidants contained in green tea, exert various actions such as anti-inflammatory, anti-bacterial, and anti-cancer actions. In this study, we investigated the effect of (+)-catechin on apoptosis induced by oxidative stress in fibroblasts.MethodsFibroblasts (NIH3T3) under oxidative stress induced by hydrogen peroxide (0.1 mM) were treated with either vehicle or (+)-catechin (0–100 μM). The effect of (+)-catechin on cell viability, apoptosis, phosphorylation of c-Jun terminal kinases (JNK) and p38, and activation of caspase-3 in fibroblasts under oxidative stress were evaluated.ResultsHydrogen peroxide induced apoptotic cell death in fibroblasts, accompanied by induction of phosphorylation of JNK and p38 and activation of caspase-3. Pretreatment of the fibroblasts with (+)-catechin inhibited hydrogen peroxide-induced apoptosis and reduced phosphorylation of JNK and p38 and activation of caspase-3.Conclusion(+)-Catechin protects against oxidative stress-induced cell death in fibroblasts, possibly by inhibiting phosphorylation of p38 and JNK. These results suggest that (+)-catechin has potential as a therapeutic agent for the prevention of skin aging.

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Section: Discussionmentioning

confidence: 99%

(+)-Catechin protects dermal fibroblasts against oxidative stress-induced apoptosis

Tanigawa

Kanazawa

Ichibori

et al. 2014

BMC Complement Altern Med

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“…Milk treatment can soothe irritated skin, can hydrate dry skin, and can replenish lost nutrients. It has been reported that skin wrinkles were treated with implanted autologous fibroblasts, which resulted in a significant improvement in periorbital skin flaccidity [ 33 – 35 ].…”

Section: Discussionmentioning

confidence: 99%

The Regenerative Potential of Donkey and Human Milk on the Redox-Sensitive and Proliferative Signaling Pathways of Skin Fibroblasts

Kocic

Langerholc

Kostić

et al. 2020

Oxidative Medicine and Cellular Longevity

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The influence of milk bioactive peptides on skin regenerative potential and rejuvenation is very often limited because of allergic reactions. The current study is aimed at exploring the influence of donkey colostrum and mature milk, human colostrum and mature milk, and β-casein and β-casomorphine-7, on the growth and inflammatory response of the culture of cultured skin fibroblasts exposed to these conditions for twenty-four hours. Their effects on the growth-regulatory kinases and redox-sensitive, proinflammatory transcriptional factor NF-κB were detected by using specific primary antibodies against NF-κB p65, Akt-1, phospho-Akt-1, Erk-1, phospho-Erk-1, JNK, phospho-JNK, phospho-STAT-1, and CD26, while logarithmic integrated fluorescence intensity patterns were recorded by flow cytometry. The downregulation of NF-κB p65 was observed after the exposure of skin fibroblasts to donkey milk and human colostrum, while β-casein and β-casomorphine-7 exerted the opposite effect, which suggests that noncasein bioactive peptides of donkey and human milk may be responsible for anti-inflammatory properties. The exposure to all milk species examined and β-casein leads to the activation of growth-regulatory kinases (Akt1/2/3 kinase, Erk kinase, JNK kinase, and Stat-1 kinase), especially for the p-Erk pathway, which suggests that essential amino acids of casein may be responsible for Erk-induced cell cycle activation and proliferation. The opposite effect was observed when cells were exposed to β-casomorphine-7, which may affect the skin fibroblast survival and their proliferative and regenerative potential. Donkey milk did not significantly change the CD26 antigen expression. In conclusion, our results suggest that among cell signaling molecules, the most sensitive but nonspecific downstream effector is p-Erk kinase, which may point to donkey milk usefulness in wound healing, regenerative, and aesthetic dermatology. The noncasein bioactive peptides of donkey milk may be responsible for the anti-inflammatory property of donkey milk and colostrum, which may indicate the usefulness in the treatment of inflammatory skin diseases.

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“…Beyond this however, there has been much interest in cultured stem cells and autologous cells for their use in regenerative medicine 96 . There have multiple studies demonstrating that autologous cultured skin fibroblast therapy can be used to correct rhytids and skin flaccidity 74,75 . Additional studies have even demonstrated the ability to correct skin discoloration seen with diseases such as vitiligo 76 .…”

Section: In Vitro Models a Cell Culturementioning

confidence: 99%

“…80 There have been multiple studies demonstrating that autologous cultured skin fibroblast therapy can be used to correct rhytids and skin flaccidity. 81,82 Additional studies have even demonstrated the ability to correct skin discoloration seen with diseases such as vitiligo. 83 Injected adipose-derived stem cells have been shown to increase collagen synthesis in hairless mice, improve dermal thickness, and promote angiogenesis.…”

Section: In Vitro Models Cell Culturementioning

confidence: 99%

Translational Research Techniques for the Facial Plastic Surgeon: An Overview

et al. 2023

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The field of Facial Plastic and Reconstructive Surgery (FPRS) is an incredibly diverse, multi-specialty field that seeks innovative and novel solutions for the management of physical defects on the head and neck. To aid in the advancement of medical and surgical treatments for these defects, there has been a recent emphasis on the importance of translational research. With recent technological advancements, there are now a myriad of research techniques that are widely accessible for physician and scientist use in translational research. Such techniques include integrated multiomics, advanced cell culture and microfluidic tissue models, established animal models, and emerging computer models generated using bioinformatics. The following article discusses these various research techniques and how they have and can be used for research in the context of various important diseases within the field of FPRS.

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Autologous Fibroblast Culture in the Repair of Aging Skin

Cited by 23 publications

References 17 publications

(+)-Catechin protects dermal fibroblasts against oxidative stress-induced apoptosis

(+)-Catechin protects dermal fibroblasts against oxidative stress-induced apoptosis

The Regenerative Potential of Donkey and Human Milk on the Redox-Sensitive and Proliferative Signaling Pathways of Skin Fibroblasts

Translational Research Techniques for the Facial Plastic Surgeon: An Overview

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