Autologous Fat Grafting

Kakagia, Despoina; Pallua, Norbert

doi:10.1177/1553350613518846

Cited by 98 publications

(48 citation statements)

References 86 publications

(98 reference statements)

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“…The current study suggests that the complications of immediate breast reconstruction do differ depending on approach and that radiation therapy appears to modestly increase certain risks. Further research is necessary to determine whether the increasing use of acellular dermal matrices 42 and autologous fat grafting 43 in implant-based reconstruction or the use of delayed reconstruction approaches (including a staged, “delayed-immediate” approach to autologous reconstruction with transfer of the flap after radiation) may alter these risks 9444546 .…”

Section: Discussionmentioning

confidence: 99%

Complications After Mastectomy and Immediate Breast Reconstruction for Breast Cancer

et al. 2016

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Objective To evaluate complications after post-mastectomy breast reconstruction, particularly in the setting of adjuvant radiotherapy. Summary-Background Data Most studies of complications after breast reconstruction have been conducted at centers of excellence; relatively little is known about complication rates in radiated patients treated in the broader community. This information is relevant for breast cancer patients' decision-making. Methods Using the claims-based MarketScan database, we described complications in 14,894 women undergoing mastectomy for breast cancer from 1998-2007 who received immediate autologous reconstruction (n=2637), immediate implant-based reconstruction (n=3007), or no reconstruction within the first two postoperative years (n=9250). We used a generalized estimating equation to evaluate associations between complications and radiotherapy over time. Results Wound complications were diagnosed within the first two postoperative years in 2.3% of patients without reconstruction, 4.4% with implants, and 9.5% with autologous reconstruction (p<0.001). Infection was diagnosed within the first two postoperative years in 12.7% of patients without reconstruction, 20.5% with implants, and 20.7% with autologous reconstruction (p<0.001). 5219 (35%) women received radiation. Radiation was not associated with infection in any surgical group within the first six months but was associated with an increased risk of infection in months 7-24 in all three groups (each p<0.001). In months 7-24, radiation was associated with higher odds of implant removal in patients with implant reconstruction (OR 1.48, p<0.001) and fat necrosis in those with autologous reconstruction (OR=1.55; P=0.01). Conclusions Complication risks after immediate breast reconstruction differ by approach. Radiation therapy appears to modestly increase certain risks, including infection and implant removal.

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Section: Discussionmentioning

confidence: 99%

Complications After Mastectomy and Immediate Breast Reconstruction for Breast Cancer

et al. 2016

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“…The increased vacuum increases the aspiration rate; however, a very high pressure may disrupt structural integrity of ASCs and other cells [76,77]. Additionally, cannula size and types of syringe needles also might affect the cell yield, size, and viability of harvested fat [78,79,80].…”

Section: Adipose Tissues As Source Of Ascsmentioning

confidence: 99%

Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells

Dubey

Mishra²,

Dubey

et al. 2018

IJMS

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Adipose-derived stromal/stem cells (ASCs) seems to be a promising regenerative therapeutic agent due to the minimally invasive approach of their harvest and multi-lineage differentiation potential. The harvested adipose tissues are further digested to extract stromal vascular fraction (SVF), which is cultured, and the anchorage-dependent cells are isolated in order to characterize their stemness, surface markers, and multi-differentiation potential. The differentiation potential of ASCs is directed through manipulating culture medium composition with an introduction of growth factors to obtain the desired cell type. ASCs have been widely studied for its regenerative therapeutic solution to neurologic, skin, wound, muscle, bone, and other disorders. These therapeutic outcomes of ASCs are achieved possibly via autocrine and paracrine effects of their secretome comprising of cytokines, extracellular proteins and RNAs. Therefore, secretome-derivatives might offer huge advantages over cells through their synthesis and storage for long-term use. When considering the therapeutic significance and future prospects of ASCs, this review summarizes the recent developments made in harvesting, isolation, and characterization. Furthermore, this article also provides a deeper insight into secretome of ASCs mediating regenerative efficacy.

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“…To the best of our knowledge, there are few publications of a prospective and controlled study investigating the use of lipofilling breast volumetry in the field of breast reconstruction. Careful analysis of the literature highlights the presence of several approaches to fat graft differing in harvesting, centrifugation or infiltration methods [32,33,34,35,36]. In particular, some authors strongly support the potential of stem cells for the purpose of increasing fat graft survival, indicating the enrichment of lipofilling with stem cells as the best alternative to major tissue augmentation [37,38].…”

Section: Discussionmentioning

confidence: 99%

The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo

Francesco

Guastafierro

Nicoletti

et al. 2017

IJMS

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Autologous fat grafting procedures in plastic surgery have been extensively used to reinforce soft tissue in congenital or acquired tissue impairments. With this background, the aim of this study is firstly to examine the impact of a selective centrifugation on existing adipose stem cells (ASCs) in terms of stemness profile maintenance and, secondly, to investigate the effect of restoring volume in reconstruction on patients affected by soft tissue damage. After centrifugation, the fat graft products were separated into two layers and subsequently examined in vitro for the expression of CD34, CD90, CD117, CD105, CD29, CD31, CD44, CD73, CD133, CD14 and CD45 markers by flow cytometry and gene expression analyses were performed for Sox2, WNT3A, END, CD44, FUT4, COLL1, CTNNB1, hbEGF, KRTLG, MMP2 and VIM genes. The results showed that in the middle-high density (MHD) layer there was a peak concentration of ASCs, compared to another layer obtained after centrifugation. Research carried out on patients under treatment for soft tissue regeneration using cells obtained from MHD layer selection will be fundamental in comparative analysis. These studies will lead to an adequate standardization of outcomes, provided that treatment is performed through cell selection. Therefore, a unique procedure in tissue reconstruction and regeneration through fat grafting is presented here.

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Autologous Fat Grafting

Cited by 98 publications

References 86 publications

Complications After Mastectomy and Immediate Breast Reconstruction for Breast Cancer

Complications After Mastectomy and Immediate Breast Reconstruction for Breast Cancer

Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells

The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo

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