Autologous chondrocytes versus filtered bone marrow mesenchymal stem/stromal cells for knee cartilage repair—a prospective study

Martinčič, David; Leban, Jure; Filardo, Giuseppe; Busacca, Maurizio; Barlič, Ariana; Veber, Matija; Drobnič, Matej

doi:10.1007/s00264-020-04727-2

Cited by 13 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, only five studies, all in the knee joint, have reported some cellular parameters of intraoperatively applied BMAC. 7,36–39 In 2 case series, Gobbi et al documented the concentration of CFU-F per milliliter (CFU-F/mL) that ranged between 2000 and 5700/mL, which is close to the mean concentration of 8811.5/mL observed in the present cohort. 36,37 Skowronski and Rutka reported only the TNC per milliliter (TNC/mL), ranging from 4.5 × 10 5 to 9 × 10 5 cells/mL, which is approximately 100-fold less than the mean TNC concentration of 125.8 × 10 6 /mL in the current group.…”

Section: Discussionsupporting

confidence: 83%

“…38 In Martinčič et al study, the final products contained on average 1156 CFU-F, while Veber et al performed the most detailed evaluation of the final cell suspensions and their processing, reporting the mean dosage of 1571 CFU-F administered to the patients. 7,39 However, there have not been analysed any cellular parameters of the intraoperatively applied BMAC as possible prognostic factors for the treatment outcomes. In the present study, the regression models identified some cellular parameters that influence the treatment outcomes.…”

Section: Parameters Of Fbma Mean (Sd)mentioning

confidence: 99%

See 1 more Smart Citation

Biomaterials augmented with filtered bone marrow aspirate for the treatment of talar osteochondral lesions. A comparison of clinical and cellular parameters

Kolar,

Veber,

Girandon

et al. 2024

J Orthop Surg (Hong Kong)

Self Cite

View full text Add to dashboard Cite

Background Biomaterials augmented with Bone Marrow Aspirate Concentrate (BMAC) are becoming increasingly utilized in the cartilage treatment. However, the potential role of cellular parameters in the intraoperatively applied BMAC have yet to be elucidated. Purpose (A) To evaluate clinical outcomes and safety of a combined single-step approach with scaffolds (fibrin glues, collagen gels, collagen-hydroxyapatite membrane) and filtered Bone Marrow Aspirate (fBMA) for the treatment of osteochondral lesions of the talus (OLTs). (B) To identify significant factors for postoperative improvements, considering cellular parameters as potential predictors. Methods All the patients operated on due to OLTs by the combination above were selected from the hospital registry database (35 pts, years 16–55, and minimally 1 year follow-up). Treatment outcomes were followed clinically with Patient-reported outcome measures (PROMs), and by pursuing serious adverse events (SAE) and graft failures (GF). Cellular parameters of the injected fBMA were determined. Pre- and postoperative PROMs values were compared to evaluate postoperative improvements. Multivariable regression models were applied to identify potential factors (demographics, medical history, joint and lesion characteristics, scaffold type, surgical and cellular parameters) that predict the treatment outcomes. Results At the mean follow-up of 32.2 (12.5) months, all Foot and Ankle Outcome Score (FAOS) and European Quality of Life in Five Dimensions Three-Level (EQ-5D-3 L) values improved significantly. 4 (11%) SAE (3 arthrofibrosis, one hardware removal), and 3 (9%) GF occurred. Female gender and concomitant procedures were the main negative predictors for postoperative outcomes. The number of fibroblast colony forming units (CFU-F) or their proportion among total nucleated cells (CFU-F/TNC) were positively correlated with the improvements of some PROMs. Conclusions Scaffolds augmented with fBMA proved as an adequate and safe approach for OLTs treatment. Cellular parameters seem to influence the treatment outcomes, thus further attention should be given to the intraoperatively applied products. Level of evidence Level IV.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Parameters Of Fbma Mean (Sd)mentioning

confidence: 99%

Biomaterials augmented with filtered bone marrow aspirate for the treatment of talar osteochondral lesions. A comparison of clinical and cellular parameters

Kolar,

Veber,

Girandon

et al. 2024

J Orthop Surg (Hong Kong)

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, comparative studies among osteochondral scaffolds could help improving the field. Finally, while these cell‐free scaffolds have been developed to overcome the problems related to cell‐expansion, the possibility to augment them with cell concentrates, to exploit their regenerative and homeostatic potential [27, 55, 63], in one‐step procedures should be explored to help addressing challenging lesions such as those presenting an OA joint environment.…”

Section: Discussionmentioning

confidence: 99%

Multi-layer cell-free scaffolds for osteochondral defects of the knee: a systematic review and meta-analysis of clinical evidence

et al. 2021

View full text Add to dashboard Cite

Purpose The aim of this study was to analyze the clinical results provided by multi-layer cell-free scaffolds for the treatment of knee osteochondral defects. Methods A systematic review was performed on PubMed, Web of Science, and Cochrane to identify studies evaluating the clinical efficacy of cell-free osteochondral scaffolds for knee lesions. A meta-analysis was performed on articles reporting results of the International Knee Documentation Committee (IKDC) and Tegner scores. The scores were analyzed as improvement from baseline to 1, 2, and ≥ 3 years of follow-up. The modified Coleman Methodology Score was used to assess the study methodology. Results A total of 34 studies (1022 patients) with a mean follow-up of 35 months was included. Only three osteochondral scaffolds have been investigated in clinical trials: while TruFit® has been withdrawn from the market for the questionable results, the analysis of MaioRegen and Agili-C™ provided clinical improvements at 1, 2, and ≥ 3 years of follow-up (all significantly higher than the baseline, p < 0.05), although with a limited recovery of the sport-activity level. A low rate of adverse events and an overall failure rate of 7.0% were observed, but the overall evidence level of the available studies is limited. Conclusions Multi-layer scaffolds may provide clinical benefits for the treatment of knee osteochondral lesions at short- and mid-term follow-up and with a low number of failures, although the sport-activity level obtained seems to be limited. Further research with high-level studies is needed to confirm the role of multi-layer scaffold for the treatment of knee osteochondral lesions.

show abstract

“…Autologous chondrocytes are considered a source of primary cells for the regeneration of defective and damaged cartilage and are used clinically. However, complications at the site of sampling and loss of its phenotype are problems with the use of these cells (9). As a result, there is a need to use methods that have high potential in the treatment of cartilage defects and help regenerate cartilage lesions (10).…”

Section: Introductionmentioning

confidence: 99%

The effect of synthesized cartilage tissue from human adipose-derived mesenchymal stem cells in orthopedic spine surgery in patients with osteoarthritis

Weijie

Guan²,

Jingqi

et al. 2021

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

Osteoarthritis is a joint disease that causes degeneration of articular cartilage and involvement of subcutaneous bone and inflammation of surrounding tissues. It can affect any joints, but the most common joints are the joints of the hands, feet, knees, thighs, and spine. Osteoarthritis patients need surgery in acute cases. The use of methods that increase the efficiency of this surgery has always been considered by researchers and surgeons. For this purpose, in the current study, the effect of synthesized cartilage tissue from human adipose-derived mesenchymal stem cells was considered in orthopedic spine surgery in patients with osteoarthritis. Thirty patients over the age of 60 who had acute spinal osteoarthritis and required surgery were selected. The pellet culture system of human adipose-derive mesenchymal stem cells of each patient was used to construct cartilage tissue. For 15 of them, in addition to implants, cartilage grafts were transplanted during surgery. All patients were monitored by the Oswestry Disability Index questionnaire, for one year. In general, the results showed that over time, patients with transplanted cartilage tissue and implants were in a better condition than patients who underwent only implant surgery.

show abstract

Autologous chondrocytes versus filtered bone marrow mesenchymal stem/stromal cells for knee cartilage repair—a prospective study

Cited by 13 publications

References 47 publications

Biomaterials augmented with filtered bone marrow aspirate for the treatment of talar osteochondral lesions. A comparison of clinical and cellular parameters

Biomaterials augmented with filtered bone marrow aspirate for the treatment of talar osteochondral lesions. A comparison of clinical and cellular parameters

Multi-layer cell-free scaffolds for osteochondral defects of the knee: a systematic review and meta-analysis of clinical evidence

The effect of synthesized cartilage tissue from human adipose-derived mesenchymal stem cells in orthopedic spine surgery in patients with osteoarthritis

Contact Info

Product

Resources

About