Autologous Cell-Enriched Fat Grafting for Breast Augmentation

Abstract

Autologous fat grafting for breast augmentation has faced some historical hurdles. However, in recent years it has been gaining acceptance from the medical community. This prospective, nonrandomized open-label study of 20 Japanese women supports the use of autologous fat grafting in breast augmentation and explores enhancement of fat graft tissue with autologous adipose-derived regenerative cells (ADRCs). After adipose harvesting using syringe liposuction, the tissue is processed in the Celution 800 System(®), which washes the graft and isolates ADRCs. The average cells per gram of harvested adipose tissue was 3.42 × 10(5), and the mean cell viability measured using an automated cell counting system before graft delivery was 85.3%. All patients demonstrated improvement in circumferential breast measurement (BRM) from their baseline state, and breast measurements were stable by 3 months after surgery. The mean BRM 9 months after surgery had increased 3.3 cm from preoperative measurements. Through 9 months, overall physician satisfaction was 69% and patient satisfaction was 75%. No serious or unexpected adverse events were reported, and the procedure was safe and well tolerated in all patients. Postoperative cyst formation was seen in two patients. These prospective results demonstrate that ADRC-enriched fat grafts processed with a closed automated system maintain high cell viability and that the procedure is safe and effective, with all patients showing improvement after a single treatment.

Adipose-Derived Stem Cells: In Musculoskeletal Disorders

“…Besides, they have several disadvantages such as high cost, immunogenicity and allergenicity, and the risk of transmitting infectious diseases. Although, autologous fat grafts including ADSC [25] are in current clinical practice, their long-term graft retention [26] [27][28][29][30]. Alternatively, Kim and his coworkers were successful in transplanting the differentiating adipocytes from ADSC for treating depressed scar with up to 75 % success rate [31].…”

Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements

“…In the last five years, a new approach has been developed to improve fat grafting through the addition of stem cells or growth factors [25,31,34,35,37,39]. In all of these protocols, whether the addition of PRP, SVF, or ASC is implied, the supra-physiological concentrations of stem cells and growth factors is a sensitive issue in reconstructive breast surgery with a risk of cancer relapse [72].…”

Adipose-Derived Stem Cells (ASC) Communicate with Residual Breast Cancer Cells

“…Several studies indicate that adipose-derived stem cells enhance the angiogenesis, prohibit the apoptosis and support the differentiation of the adipocytes by secretion of growth factors, cytokines and chemokines [6,9,10]. The understanding that the enrichment of grafts with adipose-derived stem cells improves the clinical efficiency has led to the innovative use of ASC in post-oncological breast reconstruction.…”