Autologous cartilage fragments in a composite scaffold for one stage osteochondral repair in a goat model

Marmotti, Antonio; Bruzzone, Matteo; Bonasia, Davide Edoardo; Castoldi, Filippo; Degerfeld, Mitzy Mauthe; Bignardi, Cristina; Mattia, S.; Maiello, A.; Rossi, Roberto; Peretti, G.

doi:10.22203/ecm.v026a02

Cited by 44 publications

(38 citation statements)

References 55 publications

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“…The importance of removing the calcified cartilage layer for the formation of repair tissue by the original microfracturing technique has recently been demonstrated in a horse model . Thus, a significant portion of the cells within the forming repair tissue of recent successful repair approaches may not origin from the cartilage grafts themselves but rather from the bone marrow. Nevertheless, transplanted cartilage grafts may exert an immanent beneficial role by supporting chondrogenic differentiation of ingrowing mesenchymal repair cells, since cartilage tissue or chondrocytes were shown to exert significant pro‐chondrogenic paracrine effects …”

Section: Discussionmentioning

confidence: 99%

“…[5][6][7] In rabbit and goat models, transplanted native cartilage fragments contributed to the formation of better repair tissue in treated cartilage defects than application of the scaffolds alone. 6,[8][9][10] Successful results following transplantation of autologous cartilage fragments or allogeneic particulated juvenile articular cartilage were also observed in large animal models 11 and human studies. 12,13 A variety of biodegradable scaffolds have been used for embedding the cartilage fragments.…”

mentioning

confidence: 91%

“…Indeed, cell outgrowth from cartilage tissue was demonstrated in vitro and in vivo . In rabbit and goat models, transplanted native cartilage fragments contributed to the formation of better repair tissue in treated cartilage defects than application of the scaffolds alone . Successful results following transplantation of autologous cartilage fragments or allogeneic particulated juvenile articular cartilage were also observed in large animal models and human studies .…”

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confidence: 96%

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Limited integrative repair capacity of native cartilage autografts within cartilage defects in a sheep model

Gelse

Riedel

Pachowsky

et al. 2014

Journal Orthopaedic Research

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The purpose of this study was to investigate integration and cellular outgrowth of native cartilage autografts transplanted into articular cartilage defects. Native cartilage autografts were applied into chondral defects in the femoral condyle of adult sheep. Within the defects, the calcified cartilage layer was either left intact or perforated to induce bone marrow stimulation. Empty defects served as controls. The joints were analyzed after 6 and 26 weeks by macroscopic and histological analysis using the ICRS II Score and Modified O'Driscoll Scores. Non-treated defects did not show any endogenous regenerative response and bone marrow stimulation induced fibrous repair tissue. Transplanted native cartilage grafts only insufficiently integrated with the defect borders. Cell death and loss of proteoglycans were present at the margins of the grafts at 6 weeks, which was only partially restored at 26 weeks. Significant cellular outgrowth from the grafts or defect borders could not be observed. Bonding of the grafts could be improved by additional bone marrow stimulation providing ingrowing cells that formed a fibrous interface predominantly composed of type I collagen. Transplanted native cartilage grafts remain as inert structures within cartilage defects and fail to induce integrative cartilage repair which rather demands additional cells provided by additional bone marrow stimulation. ß

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 96%

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Limited integrative repair capacity of native cartilage autografts within cartilage defects in a sheep model

Gelse

Riedel

Pachowsky

et al. 2014

Journal Orthopaedic Research

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show abstract

“…The other primary focus of laboratory research has been the development of a composite scaffold for the delivery of autologous particulated cartilage [15][16][17]. The authors demonstrated in a rabbit model that chondral defects of the knee could be effectively treated with autologous particulated cartilage delivered in a composite scaffold made of hyaluronic acid felt, fibrin glue, and platelet rich plasma [16].…”

Section: Laboratory Researchmentioning

confidence: 99%

“…The authors demonstrated in a rabbit model that chondral defects of the knee could be effectively treated with autologous particulated cartilage delivered in a composite scaffold made of hyaluronic acid felt, fibrin glue, and platelet rich plasma [16]. These results were then replicated in a goal model [17]. However, they have not shown superiority of this technique to simple transplantation of autologous particulated cartilage in fibrin glue.…”

Section: Laboratory Researchmentioning

confidence: 99%

Particulated articular cartilage for symptomatic chondral defects of the knee

Riboh

Cole

Farr³

2015

Curr Rev Musculoskelet Med

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The treatment of focal cartilage defects in the knee remains a challenging clinical problem. One relatively new unique treatment option is particulated articular cartilage, which includes autograft and off-the-shelf allogeneic juvenile grafts. The use of particulated cartilage has the advantage of being a single-stage procedure. In the case of autograft, it is cost efficient, while in the juvenile allograft form, it may have increased proliferative and restorative potentials. Laboratory and clinical data are limited for particulated cartilage grafts; however, there are promising histologic and clinical outcomes. This review provides a summary of the indications, surgical technique, and most up-to-date research on particulated cartilage for the repair of symptomatic chondral defects in the knee.

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Enhancing Biological and Biomechanical Fixation of Osteochondral Scaffold: A Grand Challenge

Tamaddon

Liu

2018

Advances in Experimental Medicine and Biology

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Osteoarthritis (OA) is a degenerative joint disease, typified by degradation of cartilage and changes in the subchondral bone, resulting in pain, stiffness and reduced mobility. Current surgical treatments often fail to regenerate hyaline cartilage and result in the formation of fibrocartilage. Tissue engineering approaches have emerged for the repair of cartilage defects and damages to the subchondral bones in the early stage of OA and have shown potential in restoring the joint's function. In this approach, the use of three-dimensional scaffolds (with or without cells) provides support for tissue growth. Commercially available osteochondral (OC) scaffolds have been studied in OA patients for repair and regeneration of OC defects. However, some controversial results are often reported from both clinical trials and animal studies. The objective of this chapter is to report the scaffolds clinical requirements and performance of the currently available OC scaffolds that have been investigated both in animal studies and in clinical trials. The findings have demonstrated the importance of biological and biomechanical fixation of the OC scaffolds in achieving good cartilage fill and improved hyaline cartilage formation. It is concluded that improving cartilage fill, enhancing its integration with host tissues and achieving a strong and stable subchondral bone support for overlying cartilage are still grand challenges for the early treatment of OA.

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Autologous cartilage fragments in a composite scaffold for one stage osteochondral repair in a goat model

Abstract: We propose a culture-free approach to osteochondral repair with minced autologous cartilage fragments loaded onto a scaffold composed of a hyaluronic acid (HA)-derived membrane, platelet-rich fibrin matrix (PRFM) and fibrin glue. The aim of the study was to demonstrate in vitro

Cited by 44 publications

References 55 publications

Limited integrative repair capacity of native cartilage autografts within cartilage defects in a sheep model

Limited integrative repair capacity of native cartilage autografts within cartilage defects in a sheep model

Particulated articular cartilage for symptomatic chondral defects of the knee

Enhancing Biological and Biomechanical Fixation of Osteochondral Scaffold: A Grand Challenge

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