Autologous Blood-Derived Patches Used as Anti-adhesives in a Rat Uterine Horn Damage Model

An autologous blood-derived patch as a hemostatic agent: evidence from thromboelastography experiments and a porcine liver punch biopsy model

“…To our knowledge this is the first preclinical study of a fully autologous blood-derived patch being used for hemostasis, either in vitro or in vivo. Through in vivo pilot studies, a recently published rat study [ 16 ] and this study, we found that the autologous blood-derived patch has limitations regarding the lack of adhesion and need for pre-preparation. In pilot studies and in our rat study [ 16 ] we observed that the patch did not adhere inadvertently to the surface of undamaged tissue or metal; however, more importantly there was longer lasting adherence to damaged tissues, and especially to blood.…”

“…Through in vivo pilot studies, a recently published rat study [ 16 ] and this study, we found that the autologous blood-derived patch has limitations regarding the lack of adhesion and need for pre-preparation. In pilot studies and in our rat study [ 16 ] we observed that the patch did not adhere inadvertently to the surface of undamaged tissue or metal; however, more importantly there was longer lasting adherence to damaged tissues, and especially to blood. This selective adhesion effect might be positive, acknowledging that inappropriate adhesion could be a problem in cases where the surgeons position the patch through laparoscopy trocars, shift the patch between instruments or need to reposition the hemostatic patch.…”

“…In our opinion the hemostatic effect of the patch is most important. The patch staying in place after obtaining hemostasis is less important, and the presence of the patch in the abdominal cavity is expected to be innocuous based on a former study [ 16 ].…”

“…The resulting patch is a three-layered leukocyte, platelet, and fibrin construct. The patch is currently used in the clinic for hard-to-heal ulcers [ 18 , 19 ] and has been described earlier in several studies [ 16 , 17 , 34 , 35 ].…”

“…In our group, we have searched for an alternative material for use in surgery. In this context we have worked with an autologous blood-derived patch and have demonstrated that the patch could function as an antiadhesive barrier in a rat model [ 16 ]. The patch (Fig.…”