Autoimmune responses of linear IgA disease: Both T cells and autoantibodies recognize the NC16A domain of BP180

Lin, Mong-Shang; Swartz, Susan J.; Gharia, Manish; Fairley, Janet A.; Giudice, George J.; Díaz, Luis A.

doi:10.1016/s0923-1811(98)83061-2

Cited by 2 publications

(4 citation statements)

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“…BP180‐reactive IgA autoantibodies were detected not only in most of the tested LABD sera but also in 10 of 10 BP sera and seven of nine CP sera ( Table 1). The observed IgA reactivity to BP180 in LABD is in line with the recent identification of LABD‐1 as a homologue of the ECD of BP180 21 and the identification of the NC16A domain of BP180 as a site that harbours epitopes that may be recognized by both IgG and IgA autoantibodies 10,23 . The detection of BP180‐reactive IgA in most CP sera in this study is interesting as the IgA subtype is predominantly associated with mucous membranes.…”

Section: Discussionsupporting

confidence: 88%

“…The observed IgA reactivity to BP180 in LABD is in line with the recent identification of LABD-1 as a homologue of the ECD of BP180 21 and the identification of the NC16A domain of BP180 as a site that harbours epitopes that may be recognized by both IgG and IgA autoantibodies. 10,23 The detection of BP180-reactive IgA in most CP sera in this study is interesting as the IgA subtype is predominantly associated with mucous membranes. This finding is in line with the recent identification of BP180-reactive IgA in a patient with CP.…”

Section: Discussionmentioning

confidence: 83%

“… 18–20 Most LABD sera recognize a 97‐kDa protein located in the dermoepidermal BM that most probably corresponds to a proteolytic fragment of the ECD of BP180. 21 In addition, LABD sera have been found to react with distinct regions of the ECD of BP180, namely the NC16A domain, which also harbours major epitopes recognized by sera from BP patients 22,23 …”

mentioning

confidence: 99%

“…21 In addition, LABD sera have been found to react with distinct regions of the ECD of BP180, namely the NC16A domain, which also harbours major epitopes recognized by sera from BP patients. 22,23 As most patients with BP, as well as a subset of patients with CP, have IgG reactive with BP180, and as a significant number of patients with LABD shows IgA reactive with BP180, the purpose of this study was to investigate whether autoantibodies of a particular immunoglobulin isotype were restricted to a distinct clinical entity. Sera from patients with BP, CP and LABD were therefore screened by immunoblot (IB) analysis for IgG, IgA and IgE reactivity with a recombinant, baculovirus-derived protein representing the ECD of BP180.…”

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confidence: 99%

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IgG, IgA and IgE autoantibodies against the ectodomain of BP180 in patients with bullous and cicatricial pemphigoid and linear IgA bullous dermatosis

Christophoridis

Büdinger

Borradori

et al. 2000

British Journal of Dermatology

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The presence of IgG and IgA autoantibody responses to BP180 in patients with three clinically distinct autoimmune bullous diseases indicates that an autoimmune response to the same distinct adhesion protein may lead to different clinical manifestations. It is therefore conceivable that variable epitopes of BP180 are targeted by the different autoantibody isotypes, resulting in the distinct clinical pictures.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 83%

mentioning

confidence: 99%

mentioning

confidence: 99%

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IgG, IgA and IgE autoantibodies against the ectodomain of BP180 in patients with bullous and cicatricial pemphigoid and linear IgA bullous dermatosis

Christophoridis

Büdinger

Borradori

et al. 2000

British Journal of Dermatology

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Identification and Characterization of Epitopes Recognized by T Lymphocytes and Autoantibodies from Patients with Herpes Gestationis

Lin¹,

Gharia²,

Swartz³

et al. 1999

The Journal of Immunology

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Autoantibodies associated with herpes gestationis (HG), a pregnancy-associated autoimmune skin disease, target the hemidesmosomal protein BP180. It was shown that the major noncollagenous stretch of the BP180 ectodomain (NC16A) harbors epitopes recognized by HG sera. Furthermore, Abs reactive with the homologous domain of murine BP180 are known to trigger a cutaneous blistering disease in mice by passive transfer experiments. The present study was aimed at characterizing the T cell responses and specificities of autoantibodies from two HG patients. Using immunoblotting and T cell proliferation assays, we have identified a 14-amino-acid stretch of the BP180 ectodomain (MCW-1; aa 507–520) that is recognized by both T cells and autoantibodies produced by the HG patients. The neonate born to one of these HG patients showed no signs of skin disease and had no detectable T cell response to the BP180 Ag, but did have a low titer of circulating anti-BP180 autoantibodies, presumably of maternal origin. BP180-specific T cell lines and clones developed from an HG patient specifically reacted with the MCW-1 epitope. The proliferative responses of these clones were restricted to HLA-DR, but not -DQ or -DP. These Ag-specific T cells expressed α/β TCRs and a CD4 memory T cell phenotype and secreted IFN-γ and IL-2, but not IL-4 or IL-6, suggesting that they are Th1-type lymphocytes. Further characterization of these Ag-specific T cells and autoantibodies will aid in elucidating the autoimmune mechanism(s) leading to the development of HG.

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Autoimmune responses of linear IgA disease: Both T cells and autoantibodies recognize the NC16A domain of BP180

Cited by 2 publications

References 0 publications

IgG, IgA and IgE autoantibodies against the ectodomain of BP180 in patients with bullous and cicatricial pemphigoid and linear IgA bullous dermatosis

IgG, IgA and IgE autoantibodies against the ectodomain of BP180 in patients with bullous and cicatricial pemphigoid and linear IgA bullous dermatosis

Identification and Characterization of Epitopes Recognized by T Lymphocytes and Autoantibodies from Patients with Herpes Gestationis

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