Autohydrolysis of Miscanthus x giganteus for the production of xylooligosaccharides (XOS): Kinetics, characterization and recovery

Chen, Ming Hsu; Bowman, Michael J.; Dien, Bruce S.; Rausch, Kent D.; Tumbleson, Mike E; Singh, Vijay

doi:10.1016/j.biortech.2013.12.050

Cited by 70 publications

(32 citation statements)

References 29 publications

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“…The enzymatic method resulted in the production of XOS with degree of polymerisation (DP) of 2-3, while chemical hydrolysis (autohydrolysis or acid hydrolysis) produced these, but also other XOS with higher DP (2,(38)(39)(40), without prebiotic eff ect. Commercial XOS (Xylooligo 95P; Suntory, Osaka, Japan), produced by autohydrolysis, containing 83 % of xylobiose and xylotriose were used in the culture medium as carbon source.…”

Section: Discussionmentioning

confidence: 99%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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Currently, lignocellulosic waste is a topic of global studies, given that fossil fuel reserves are diminishing, the new agricultural frontiers are limited and the demand for food and biofuels is increasing by the growing world population (1). For these reasons, technology must advance to improve the use of agricultural and agroindustrial residues to obtain food and biofuel.Xylans present in lignocellulosic materials have been studied for obtaining xylooligosaccharides (XOS) from waste materials such as corncobs, rice hulls, olive pits, barley straw (2), tobacco stalk, cott on stalk, sunfl ower stalk, wheat straw (3) and sugarcane bagasse (4-6). Sugarcane bagasse is inexpensive, renewable and abundant source of XOS especially in the countries that produce ethanol and sugar from sugarcane. However, more research is necessary to improve the use of this residue. SummarySugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (XOS). Some XOS are considered soluble dietary fi bre, with low caloric value and prebiotic eff ect, but they are expensive and not easily available. In a screening of 138 fungi, only nine were shortlisted, and just Aspergillus fumigatus M51 (35.6 U/mL) and A. fumigatus U2370 (28.5 U/mL) were selected as the most signifi cant producers of xylanases. These fungi had low β-xylosidase activity, which is desirable for the production of XOS. The xylanases from Trichoderma reesei CCT 2768, A. fumigatus M51 and A. fumigatus U2370 gave a signifi cantly higher XOS yield, 11.9, 14.7 and 7.9 % respectively, in a 3-hour reaction with hemicellulose from sugarcane bagasse. These enzymes are relatively thermostable at 40-50 °C and can be used in a wide range of pH values. Furthermore, these xylanases produced more prebiotic XOS (xylobiose and xylotriose) when compared with a commercial xylanase. The xylanases from A. fumigatus M51 reached a high level of XOS production (37.6 %) in 48-72 h using hemicellulose extracted from sugarcane bagasse. This yield represents 68.8 kg of prebiotic XOS per metric tonne of cane bagasse. In addition, in a biorefi nery, aft er hemicellulose extraction for XOS production, the residual cellulose could be used for the production of second-generation ethanol.

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Section: Discussionmentioning

confidence: 99%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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“…En la figura 1a se presenta el efecto del tiempo sobre la liberación de los ART por acción del PTH empleando sólo agua. En esta primera prueba se fijó una temperatura de 175 ºC, la cual es cercana a la empleada en el PTH de biomasa (Chen et al 2014, Gonçalves et al 2015, Vallejos et al 2015. Como se puede observar, entre los tiempos de tratamiento de 5 y 15 min no se detectó una diferencia significativa, por lo cual se eligió el tiempo de 5 min (por ahorro de energía) para estudiar el efecto de la temperatura sobre la liberación de ART.…”

Section: Comparación Del Pth Sin áCido Y Con áCido Diluidounclassified

EFECTO DEL PRETRATAMIENTO HIDROTÉRMICO (ALTA PRESIÓN) E HIDRÓLISIS ENZIMÁTICA DE CLADODIOS DE Opuntia ficus-indica SOBRE LA LIBERACIÓN DE AZÚCARES Y SU USO POTENCIAL EN LA PRODUCCIÓN DE BIOETANOL

Texco-López¹,

Álvarez-Cervantes²,

Cadena-Ramírez³

et al. 2019

Rev. Int. Contam. Amb.

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“…Reactors were capped with 19.1 mm, 316 stainless steel Swagelok caps (SS-1210-C, Swagelok) on both ends. The procedure was essentially as described by Chen et al (2014), where the loaded reactors were immersed in a fluidized sand bath (IFB-51 Industrial Fluidized Bath, Techne Inc., Burlington, NJ) preheated to 180°C and cooled in flowing room temperature tap water. Autohydrolysis was performed at water to solid ratio of 9:1, at 180°C for 20 min.…”

Section: Autohydrolysis and Sample Preparationmentioning

confidence: 99%

“…Activated carbon adsorption and ethanol elution was also used to recover XOS from MxG hydrolyzates (Chen et al, 2014). However, both Gullón et al (2010) and Chen et al (2014) recovered XOS mixtures, rather than purified oligomers that have a single degree of polymerization (DP).…”

Section: Introductionmentioning

confidence: 99%

Separation of xylose oligomers from autohydrolyzed Miscanthus×giganteus using centrifugal partition chromatography

Chen

Rajan

Carrier

2015

Food and Bioproducts Processing

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Autohydrolysis of cellulosic materials for saccharification generates xyloseoligosaccharides (XOS), due to the partial hydrolysis of xylan. Developing an efficient method for the separation and recovery of XOS from the prehydrolyzates would provide an excellent opportunity for the better utilization of the cellulosic material and for value-added co-product production. In this study, we investigated the use of centrifugal partition chromatography (CPC) for the fractionation of XOS from Miscanthus x giganteus (MxG). During autohydrolysis of miscanthus biomass at 180°C for 20 min, 63 % of xylan was converted into XOS and xylose. The ensuing XOS concentrate contained up to 30 % of XOS, which were distributed as 15.9 % xylobiose (DP2), 5.9 % xylotriose, (DP3), 5.6 % xylotetraose (DP4), 0.8 % xylopentaose (DP5) and 0.6 % xylohexaose (DP6). The XOS concentrate was further fractionated by CPC with a solvent system composed of 4:1:4 (v/v/v) butanol: methanol: water. Using CPC techniques, 230 mg (80 %) of DP2 to DP6 oligomers were fractionated from 1 g of XOS concentrate. The recoveries of individual XOS were 90.2 % DP2, 64.5 % DP3, 71.2 % DP4, 61.9 % DP5 and 68.9 % DP6. The purities of DP2 to DP6 fractions were 61.9 %, 63.2 %, 44.5 %, 31.5 % and 51.3 %, respectively. Presence of DP2 and DP3 in the CPC purified fractions was further validated by mass spectrometry analysis. The study provided information on fast recovery of individual XOS from crude biomass prehydrolyzate.

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Autohydrolysis of Miscanthus x giganteus for the production of xylooligosaccharides (XOS): Kinetics, characterization and recovery

Cited by 70 publications

References 29 publications

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

EFECTO DEL PRETRATAMIENTO HIDROTÉRMICO (ALTA PRESIÓN) E HIDRÓLISIS ENZIMÁTICA DE CLADODIOS DE Opuntia ficus-indica SOBRE LA LIBERACIÓN DE AZÚCARES Y SU USO POTENCIAL EN LA PRODUCCIÓN DE BIOETANOL

Separation of xylose oligomers from autohydrolyzed Miscanthus×giganteus using centrifugal partition chromatography

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