Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces

Kompauer, Mario; Heiles, Sven; Spengler, Bernhard

doi:10.1038/nmeth.4433

Cited by 122 publications

(121 citation statements)

References 25 publications

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“…The fluorescent‐tagged antibody specific to proteins has been the major tool for localization studies . Recently, the integration of mass spectrometry with imaging has given rise to a new field of mass spectrometry imaging (MSI) . Utilizing MSI, various types of biomolecules, including proteins and lipids, can be mapped over the surface being visualized under the microscope.…”

Section: Targeted Proteomics: What Does It Bring For Researchers At Bmentioning

confidence: 99%

“…Utilizing MSI, various types of biomolecules, including proteins and lipids, can be mapped over the surface being visualized under the microscope. Till now, major advancements have been in the profiling‐based approach with a combination of microscopy and matrix‐associated laser desorption and ionization (MALDI) or secondary ionization mass spectrometry (SIMS) . An integration of microscopy and MRM is a promising combination for the measurement of a specific set of proteins on the histopathological tissue of a diseased condition.…”

Section: Targeted Proteomics: What Does It Bring For Researchers At Bmentioning

confidence: 99%

“…[73] Recently, the integration of mass spectrometry with imaging has given rise to a new field of mass spectrometry imaging (MSI). [74,75] Utilizing MSI, various types of biomolecules, including proteins and lipids, can be mapped over the surface being visualized under the microscope. Till now, major advancements have been in the profiling-based approach with a combination of microscopy and matrixassociated laser desorption and ionization (MALDI) or secondary ionization mass spectrometry (SIMS).…”

Section: Mass Spectrometry Imaging: Targeted Proteomics To Look Insidmentioning

confidence: 99%

See 2 more Smart Citations

Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us?

Arora

Somasundaram

2019

BioEssays

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While mass spectrometry (MS)‐based quantification of small molecules has been successfully used for decades, targeted MS has only recently been used by the proteomics community to investigate clinical questions such as biomarker verification and validation. Targeted MS holds the promise of a paradigm shift in the quantitative determination of proteins. Nevertheless, targeted quantitative proteomics requires improvisation in making sample processing, instruments, and data analysis more accessible. In the backdrop of the genomic era reaching its zenith, certain questions arise: is the proteomic era about to come? If we are at the beginning of a new future for protein quantification, are we prepared to incorporate targeted proteomics at the benchside for basic research and at the bedside for the good of patients? Here, an overview of the knowledge required to perform targeted proteomics as well as its applications is provided. A special emphasis is placed on upcoming areas such as peptidomics, proteoform research, and mass spectrometry imaging, where the utilization of targeted proteomics is expected to bring forth new avenues. The limitations associated with the acceptance of this technique for mainstream usage are also highlighted. Also see the video abstract here https://youtu.be/mieB47B8gZw.

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Section: Targeted Proteomics: What Does It Bring For Researchers At Bmentioning

confidence: 99%

Section: Targeted Proteomics: What Does It Bring For Researchers At Bmentioning

confidence: 99%

Section: Mass Spectrometry Imaging: Targeted Proteomics To Look Insidmentioning

confidence: 99%

See 1 more Smart Citation

Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us?

Arora

Somasundaram

2019

BioEssays

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“…[10] Furthermore,a tmospheric near-field laser ablation coupled with MS has achieved al ateral resolution of 200 nm for easy-to-vaporize compounds. [12] Recently,a na utofocusing setup has been introduced for measuring the 3D chemical topography of non-flat sample surfaces,but only micrometerscale height variations can be resolved. This comprehensive information facilitates the visualization of 3D topographically reconstructed distributions of chemical species in subcellular regions.…”

mentioning

confidence: 99%

“…This comprehensive information facilitates the visualization of 3D topographically reconstructed distributions of chemical species in subcellular regions. [12] Recently,a na utofocusing setup has been introduced for measuring the 3D chemical topography of non-flat sample surfaces,but only micrometerscale height variations can be resolved. [12b] No single analytical technique is available that can provide the desired multimodal characterization abilities for topographically reconstructed single-cell imaging at submicrometer-scale lateral resolution.…”

mentioning

confidence: 99%

Chemical and Topographical Single‐Cell Imaging by Near‐Field Desorption Mass Spectrometry

et al. 2019

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Simultaneously acquiring chemical and topographical information within asingle cell at nanoscale resolutions is vital to cellular biology,yet it remains agreat challenge due to limited lateral resolutions and detection sensitivities.H erein, the development of near-field desorption mass spectrometry for correlated chemical and topographical imaging is reported, therebyb ridging the gap between laser-based mass spectrometry (MS) methods and multimodal single-cell imaging.Using this integrated platform, an imaging resolution of 250 nm and 3D topographically reconstructed chemical single-cell imaging were achieved.T his technique offers more in-depth cellular information than micrometer-range laser-based MS imaging methods.C onsidering the simplicity and compact sizeo ft he near-field device,this techniquecan be introduced to MALDI-MS,e xpanding the multimodal abilities of MS at nanoscale resolutions.

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Metabolic Fingerprinting on Synthetic Alloys for Medulloblastoma Diagnosis and Radiotherapy Evaluation

et al. 2020

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Diagnostics is the key in screening and treatment of cancer. As an emerging tool in precision medicine, metabolic analysis detects end products of pathways, and thus is more distal than proteomic/genetic analysis. However, metabolic analysis is far from ideal in clinical diagnosis due to the sample complexity and metabolite abundance in patient specimens. A further challenge is real‐time and accurate tracking of treatment effect, e.g., radiotherapy. Here, Pd–Au synthetic alloys are reported for mass‐spectrometry‐based metabolic fingerprinting and analysis, toward medulloblastoma diagnosis and radiotherapy evaluation. A core–shell structure is designed using magnetic core particles to support Pd–Au alloys on the surface. Optimized synthetic alloys enhance the laser desorption/ionization efficacy and achieve direct detection of 100 nL of biofluids in seconds. Medulloblastoma patients are differentiated from healthy controls with average diagnostic sensitivity of 94.0%, specificity of 85.7%, and accuracy of 89.9%, by machine learning of metabolic fingerprinting. Furthermore, the radiotherapy process of patients is monitored and a preliminary panel of serum metabolite biomarkers is identified with gradual changes. This work will lead to the application‐driven development of novel materials with tailored structural design and establishment of new protocols for precision medicine in near future.

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Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces

Cited by 122 publications

References 25 publications

Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us?

Targeted Proteomics Comes to the Benchside and the Bedside: Is it Ready for Us?

Chemical and Topographical Single‐Cell Imaging by Near‐Field Desorption Mass Spectrometry

Metabolic Fingerprinting on Synthetic Alloys for Medulloblastoma Diagnosis and Radiotherapy Evaluation

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