1965
DOI: 10.1038/2051333a0
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Autofluorescence of Eosinophils: a Bone Marrow Study

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Cited by 29 publications
(15 citation statements)
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“…Endobronchial biopsy specimens were obtained 48 hours after antigen (and normal saline) challenge. Specific autofluorescence imaging of eosinophil peroxidase in endobronchial biopsies was performed as described elsewhere (38)(39)(40). EPO-specific in situ peroxidase staining of tissues was performed after treatment of slides with 0.01 M KCN to inhibit MPO (41,42).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Endobronchial biopsy specimens were obtained 48 hours after antigen (and normal saline) challenge. Specific autofluorescence imaging of eosinophil peroxidase in endobronchial biopsies was performed as described elsewhere (38)(39)(40). EPO-specific in situ peroxidase staining of tissues was performed after treatment of slides with 0.01 M KCN to inhibit MPO (41,42).…”
Section: Methodsmentioning
confidence: 99%
“…First, endobronchial biopsy specimens were examined by fluorescence microscopy using excitation and emission wavelengths specific for the autofluorescence of the porphyrin group of EPO (38)(39)(40). Dramatic increases in fluorescence intensity were observed in allergen-challenged, but not normal saline-challenged, specimens from allergic asthmatic subjects ( Figure 3, c and d).…”
Section: Figurementioning
confidence: 99%
“…All biopsies were stained on the same day. Specific autofluorescence imaging of EPO in biopsies was performed as described (47)(48)(49). EPO-specific in situ peroxidase staining of tissues was performed on anti-nitrotyrosine-stained tissue sections following treatment of slides with 0.01 M KCN to inhibit MPO (50,51).…”
Section: Histological Analysis Of Lung and Bronchial Tissuesmentioning
confidence: 99%
“…The fluorophoreeosinophil granule complexes described in this study were clearly photoreactive, increasing their quantum emission and then stabilizing with continuous photoexcitation, whereas background Biebrich scarlet staining photobleached. This characteristic is particularly useful in eosinophils, which possess diffuse autofluorescence (520 nm) due to a granule-associated flavin adenine dinucleotide (Fuerst and Jannach 1965;Mayeno et al 1992). The masking of eosinophil autofluorescence by Biebrich scarlet not only provides definitive specific granule profiles not seen by autofluorescence but also allows dual labels with fluorescent markers that emit at this wavelength.…”
Section: Discussionmentioning
confidence: 99%