Autofluorescence of Developing Plant Vegetative Microspores Studied by Confocal Microscopy and Microspectrofluorimetry

Roshchina, Victoria V.; Yashin, V. A.; Кононов, А. В.

doi:10.1023/b:jofl.0000047225.56987.e1

Cited by 26 publications

(16 citation statements)

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“…Another strategy uses offline techniques to measure and characterize the general autofluorescence signature of selected bioaerosol types. In some studies fluorescence microscopy is used to understand general fluorescence patterns and fluorophore locations in bioaerosol proxies (e.g., Roshchina et al, 2004;Herbrich et al, 2012). Other studies have applied fluorescence spectroscopy to understand characteristic emission signatures (e.g., O'Connor et al, 2011).…”

Section: Autofluorescence In Bioaerosol Detectionmentioning

confidence: 99%

“…Fluorescence microscopy, in contrast, has been applied only occasionally for the characterization of pollen. In addition to size and shape, it provides information about surface texture and internal structures as well as spectral proper-ties (e.g., Asbeck, 1955;Driessen et al, 1989;Ronneberger et al, 2002;Roshchina et al, 2004;Scharring et al, 2006;Mitsumoto et al, 2009;Castro et al, 2010). Morphologically, it allows localization of cellular origin and estimation of relative contributions of fluorescence emission from different cellular regions (i.e., cell wall, organelles, cytosol).…”

Section: Fluorescence Microscopymentioning

confidence: 99%

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Autofluorescence of atmospheric bioaerosols: spectral fingerprints and taxonomic trends of pollen

et al. 2013

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Primary biological aerosol particles (PBAP) are important factors in atmospheric cycling, climate, and public health. Pollen is a major fraction of PBAP and is receiving increasing attention due to its high allergenic potential and the associated impacts on personal life quality and economy. Recently, autofluorescence-based techniques have proven to be valuable tools for real time, in situ quantification and classification of PBAP. First studies suggest that the autofluorescence of pollen may be sufficiently selective to be utilized for an automated and real-time monitoring of pollen in ambient air. However, the degree of selectivity autofluorescence can provide is still in question and actively debated.This study addresses the origin, properties, and selectivity of autofluorescence from natural pollen by fluorescence microscopy and spectroscopy measurements along with a systematic synthesis of related literature. We show that dry pollen reveals characteristic and reproducible autofluorescence signatures which are shaped by cell wall associated fluorophores, such as phenolic compounds and carotenoid pigments. In addition, fluorescence signals from proteins and chlorophyll a were observed in some species. The abundance and intensity of the individual fluorescence signals show certain taxonomic trends and allow systematic differentiation from bacteria and fungal spores due to the lack of proteins on the grain surface. Principal component analysis was used to explore the discrimination potential of pollen autofluorescence, in combination with size and shape, revealing a differentiation of pollen on family level. Our results help explore the levels of selectivity that autofluorescence-based techniques can provide to PBAP analysis and will support the development and application of autofluorescence-based detectors for monitoring of allergenic pollen in the atmosphere.www.atmos-meas-tech.net/6/3369/2013/ Atmos. Meas. Tech., 6, 3369-3392, 2013

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Section: Autofluorescence In Bioaerosol Detectionmentioning

confidence: 99%

Section: Fluorescence Microscopymentioning

confidence: 99%

Autofluorescence of atmospheric bioaerosols: spectral fingerprints and taxonomic trends of pollen

et al. 2013

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“…Its LIF methodology depends upon the fact that many structural components and secondary metabolites of PBAP such as tryptophan, tyrosine and NAD(P)H fluoresce (Roshchina et al, 2004(Roshchina et al, , 1995(Roshchina et al, , 1998Roshchina, 2003;Pöhlker et al, 2013). Hence the use of UV flash lamps tuned to 280 nm and 370 nm for appropriate biofluorophore excitation gives rise to emission profiles that separate them from non-fluorescent chemical particles.…”

Section: Introductionmentioning

confidence: 99%

On-line monitoring of airborne bioaerosols released from a composting/green waste site

2015

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“…It can penetrate into the cell, interact with cellular components such as DNA-containing organelles or tubulin of the cytoplasm, and selectively fluoresce upon interaction with different cellular compartments [9]. This activity makes it as valuable as other indicator fluorescent dyes.…”

Section: Introductionmentioning

confidence: 99%

Metal-enhanced S2 fluorescence from azulene

Zhang

Aslan

Previte

et al. 2006

Chemical Physics Letters

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In this paper, we report the first observation of metal-enhanced S 2 emission at room and low temperature (77K). The S 2 emission intensity of Azulene is enhanced by close proximity to Silver island films (SiFs). In this regard, a ≈ 2-fold higher S 2 fluorescence intensity of Azulene was observed from SiFs as compared to a glass control sample. This suggests that S 2 excited states can couple to surface plasmons and enhance S 2 fluorescence yields, a helpful observation in our understanding the interactions between plasmons and lumophores, and our continued efforts to develop a unified plasmon-lumophore/fluorophore theory. KeywordsS 2 emission; Metal-Enhanced S 2 Fluorescence; Radiative Decay Engineering; Surface Enhanced Fluorescence; Plasmon Controlled Fluorescence; Radiating Plasmons; Azulene; Metal-Enhanced Fluorescence IntroductionThe location of electronic energy levels in molecules and the probability and polarization of radiative transitions between ground and excited state can be accurately predicted by quantum mechanical theory [1]. For most molecules, no excited singlet states (S 2 , S 3 …) have been observed to emit light upon excitation, the same being true for triplet states (T 2 , T 3 …). This is summarized in Kasha's rule: The emitting electronic level of a given multiplicity is the lowest excited level of that multiplicity [2].As the first authentic example of a molecule which violates Kasha's rule, azulene has been the subject of numerous spectroscopic and photophysical investigations [3][4][5][6]. Beer and LonguetHiggins discovered the second excited level emits light upon excitation, producing the anomalous (S 2 to S 0 ) fluorescence of azulene in 1955 [6], whereas that normal S 1 to S 0 fluorescence and T 1 to T 0 phosphorescence were much too weak to be observed. The absence of S 1 to S 0 fluorescence suggests that S 1 to S 0 internal conversion is a very rapid progress in Azulene, this supposition being confirmed by the lifetime of the S 1 state being determined as 1.9 psec [7]. Compared with the analogous spacing of the isomer Naphthalene, Azulene has much wider spacing between its zero -point levels of the first two excited singlet states (S 1 , *Corresponding Author E-mail: geddes@umbi.umd.edu Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. [19], and the application of metallic surfaces to amplified (ultra fast and sensitive) assay detection [20]. Our laboratorys' current interpretation of MEF has been explained by a model whereby non-radiative energy transfer occurs from excited distal fluorophores, to the surface plasmon electrons in n...

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Autofluorescence of Developing Plant Vegetative Microspores Studied by Confocal Microscopy and Microspectrofluorimetry

Cited by 26 publications

References 1 publication

Autofluorescence of atmospheric bioaerosols: spectral fingerprints and taxonomic trends of pollen

Autofluorescence of atmospheric bioaerosols: spectral fingerprints and taxonomic trends of pollen

On-line monitoring of airborne bioaerosols released from a composting/green waste site

Metal-enhanced S2 fluorescence from azulene

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