2004
DOI: 10.1023/b:jofl.0000047225.56987.e1
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Autofluorescence of Developing Plant Vegetative Microspores Studied by Confocal Microscopy and Microspectrofluorimetry

Abstract: Phenomenon of autofluorescence from vegetative microspores of spore-breding plant Equisetum arvense has been studied by methods of laser-scanning confocal microscopy (LSCM) and microspectrofluorimetry during the development of the cells. The microspores have demonstrated a difference between structures: blue-fluorescing cover and red-fluorescing chloroplasts. The fluorescence spectra of the studied cells was also measured by original microspectrofluorimeter. The character of the spectra and the color of fluore… Show more

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Cited by 26 publications
(16 citation statements)
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“…Another strategy uses offline techniques to measure and characterize the general autofluorescence signature of selected bioaerosol types. In some studies fluorescence microscopy is used to understand general fluorescence patterns and fluorophore locations in bioaerosol proxies (e.g., Roshchina et al, 2004;Herbrich et al, 2012). Other studies have applied fluorescence spectroscopy to understand characteristic emission signatures (e.g., O'Connor et al, 2011).…”
Section: Autofluorescence In Bioaerosol Detectionmentioning
confidence: 99%
See 1 more Smart Citation
“…Another strategy uses offline techniques to measure and characterize the general autofluorescence signature of selected bioaerosol types. In some studies fluorescence microscopy is used to understand general fluorescence patterns and fluorophore locations in bioaerosol proxies (e.g., Roshchina et al, 2004;Herbrich et al, 2012). Other studies have applied fluorescence spectroscopy to understand characteristic emission signatures (e.g., O'Connor et al, 2011).…”
Section: Autofluorescence In Bioaerosol Detectionmentioning
confidence: 99%
“…Fluorescence microscopy, in contrast, has been applied only occasionally for the characterization of pollen. In addition to size and shape, it provides information about surface texture and internal structures as well as spectral proper-ties (e.g., Asbeck, 1955;Driessen et al, 1989;Ronneberger et al, 2002;Roshchina et al, 2004;Scharring et al, 2006;Mitsumoto et al, 2009;Castro et al, 2010). Morphologically, it allows localization of cellular origin and estimation of relative contributions of fluorescence emission from different cellular regions (i.e., cell wall, organelles, cytosol).…”
Section: Fluorescence Microscopymentioning
confidence: 99%
“…Its LIF methodology depends upon the fact that many structural components and secondary metabolites of PBAP such as tryptophan, tyrosine and NAD(P)H fluoresce (Roshchina et al, 2004(Roshchina et al, , 1995(Roshchina et al, , 1998Roshchina, 2003;Pöhlker et al, 2013). Hence the use of UV flash lamps tuned to 280 nm and 370 nm for appropriate biofluorophore excitation gives rise to emission profiles that separate them from non-fluorescent chemical particles.…”
Section: Introductionmentioning
confidence: 99%
“…It can penetrate into the cell, interact with cellular components such as DNA-containing organelles or tubulin of the cytoplasm, and selectively fluoresce upon interaction with different cellular compartments [9]. This activity makes it as valuable as other indicator fluorescent dyes.…”
Section: Introductionmentioning
confidence: 99%