2012
DOI: 10.5194/amt-5-37-2012
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Autofluorescence of atmospheric bioaerosols – fluorescent biomolecules and potential interferences

Abstract: Primary biological aerosol particles (PBAP) are an important subset of air particulate matter with a substantial contribution to the organic aerosol fraction and potentially strong effects on public health and climate. Recent progress has been made in PBAP quantification by utilizing real-time bioaerosol detectors based on the principle that specific organic molecules of biological origin such as proteins, coenzymes, cell wall compounds and pigments exhibit intrinsic fluorescence. The properties of many fluoro… Show more

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Cited by 304 publications
(404 citation statements)
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(303 reference statements)
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“…FS is fluorescence spectroscopy and FM is fluorescence microscopy. As a conventional expression (e.g., Hill et al, 2009, Pöhlker et al, 2012, Thermo Scientific Particle Technology-Product Catalog and Technical Reference Guide printing CT6000.1_1/119), we use a backslash to separate excitation and emission wavelengths (λex / λem, not to be confused with "divided by"). In addition to PSLs, polystyrene-divinylbenzene (PS-DVB) particles were also used in this study.…”
Section: Methodsmentioning
confidence: 99%
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“…FS is fluorescence spectroscopy and FM is fluorescence microscopy. As a conventional expression (e.g., Hill et al, 2009, Pöhlker et al, 2012, Thermo Scientific Particle Technology-Product Catalog and Technical Reference Guide printing CT6000.1_1/119), we use a backslash to separate excitation and emission wavelengths (λex / λem, not to be confused with "divided by"). In addition to PSLs, polystyrene-divinylbenzene (PS-DVB) particles were also used in this study.…”
Section: Methodsmentioning
confidence: 99%
“…The PS-DVB particles, which were purchased in a dry state, were measured by placing the sample directly onto a synthetic fused silica window inside the surface holder in a quantity such that the plane was fully covered. As described by Pöhlker et al (2012), fluorescent emissions from weakly fluorescent solids are qualitatively superimposed by light leakage and/or absorption effects contributing to high background signals. We therefore normalized each fluorescence matrix by the intensity of light leakage (normalization factor, NF) to make fluorescence intensities comparable along all solid samples without altering or losing actual fluorescence features.…”
Section: Fluorescence Spectroscopy On Psls In Dry Statementioning
confidence: 99%
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