Autofluorescence Imaging Reflects the Nuclear Enlargement of Tumor Cells as well as the Cell Proliferation Ability and Aberrant Status of the p53, Ki-67, and p16 Genes in Colon Neoplasms

Abstract

Background: Autofluorescence imaging (AFI) is useful for diagnosing colon neoplasms, but what affects the AFI intensity remains unclear. This study investigated the association between AFI and the histological characteristics, aberrant methylation status, and aberrant expression in colon neoplasms. Methods: Fifty-three patients with colorectal neoplasms who underwent AFI were enrolled. The AFI intensity (F index) was compared with the pathological findings and gene alterations. The F index was calculated using an image analysis software program. The pathological findings were assessed by the tumor crypt density, cell densities, and N/C ratio. The aberrant methylation of p16, E-cadherin, Apc, Runx3, and hMLH1 genes was determined by a methylation-specific polymerase chain reaction. The aberrant expression of p53 and Ki-67 was evaluated by immunohistochemical staining. Results: An increased N/C ratio, the aberrant expression of p53, Ki-67, and the altered methylation of p16 went together with a lower F index. The other pathological findings and the methylation status showed no association with the F index. Conclusions: AFI reflects the nuclear enlargement of tumor cells, the cell proliferation ability, and the altered status of cell proliferation-related genes, indicating that AFI is a useful and practical method for predicting the dysplastic grade of tumor cells and cell proliferation.

Clinicopathological value of epidermal growth factor receptor (EGFR) and Ki-67 expression in colorectal adenoma and adenocarcinoma

“…The EGFR IHC expression in colorectal adenoma case in the current study was in concordance with Williet et al 18 . In cases of investigated adenoma, the grade and type of histopathological dysplasia were significantly correlated with the Ki-67 LI, which is close to earlier findings, 19 , but findings in contrast have also been reported. 20 In the current study, 58% CRC cases showed overexpression of EGFR compared to Spano et al 15 who showed a higher percentage (80%).…”

Light and Autofluorescence, Multitasking Features in Living Organisms

“…In fact, the growing of neoplasia causes a thickening of mucosa and a disappearance of submucosa, resulting in a lowering the signal rising at the tissue surface which can be detected via endoscopy, as currently performed in the clinics by means of commercial devices [266,[325][326][327][328][329]. Notably, a direct relationship has been also reported between autofluorescence imaging data, histology and alterations in genes related to cancer cell proliferation [330].…”

LncRNA LINC01232 Enhances Proliferation, Angiogenesis, Migration and Invasion of Colon Adenocarcinoma Cells by Downregulating miR-181a-5p

“…Besides, an earlier study also proved that the expression of p53 was closely related to the invasion and lymphatic metastasis of COAD [ 26 ]. Likewise, p16 gene is also a critical tumor suppressor gene, which will lead to the proliferation of malignant cells after inactivation [ 27 ]. In line with a recent study, abnormality in the cyclinD1-CDK-p16-pRb pathway is the genetic basis of tumor development [ 28 ].…”