Autofluorescence free detection of carcinoembryonic antigen in pleural effusion by persistent luminescence nanoparticle-based aptasensors

Pan, Zaifa; Yang, Diya; Lin, Jianming; Shao, Kang; Shi, Shuxian; Teng, Yuanjie; Liu, Huijun; She, Yuanbin

doi:10.1016/j.aca.2021.339408

Cited by 12 publications

(3 citation statements)

References 33 publications

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“…Still, low-dimensional nanomaterials, such as carbon dots or graphene quantum dots (GQDs), , inorganic quantum dots, , silica-based beads, metal–organic frameworks, and noble metals, have showcased superior optical properties. In recent years, persistent luminescence nanoparticles (PLNPs) have been incorporated into CEA detection to enhance the limit of detection (LOD) because the photon lifetime of the PLNPs is longer than that of the typical fluorescence (FL). , However, the LOD is still at the scale of picograms per milliliter because the resolution is limited by the lifetime of the photon. Otherwise, the direct fluorescent signal counting is susceptible to a quenching phenomenon due to the inner filter effect (IFE) that heavily deteriorates its quantum yield and further reduces its sensitivity, accuracy, and precision.…”

Section: Introductionmentioning

confidence: 99%

“…To achieve an optimal FRET system, a fluorophore with a high quantum yield is needed to enhance the dynamic linear range of detection. Several nanomaterials like inorganic metal derivatives, , carbon dots or GQDs, , and inorganic nanodots or quantum dots , have been used as the effective FRET fluorophore for the detection of several analytes like ions, gas, and organic contaminants . GQDs are derivatives of graphene that have been widely used in biosensing due to their high quantum yield, low synthetic cost, edge effect, biocompatibility, low toxicity, photostability, and good water solubility .…”

Section: Introductionmentioning

confidence: 99%

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Composites of Gold Nanostars and Nitrogen- and Sulfur-Codoped Graphene Quantum Dots as Plasmon-Enhanced Immunosensors for Cancer Prognosis

Ng,

Lee,

Tran

et al. 2023

ACS Appl. Nano Mater.

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The prognosis of cancer by early detection of multicancer biomarkers helps in elevating the survival rate among patients, and carcinoembryonic antigen (CEA) serves as a good candidate because it is related to most cancer types. In this study, a wash-free Forster−Dexter energy-transfer (FRET−DET)-oriented immunosensor by using a nitrogen-and sulfur-codoped graphene quantum dot (NS-GQD) as the fluorophore and a plasmon-enhanced gold nanostar (GNS) as the quencher was developed for the effective and rapid detection of CEA in human serum. The NS-GQD was prepared by a one-pot hydrothermal method with L-cysteine and Lascorbic acid as the precursors. The diameter of the NS-GQD is in the range of 1−6 nm, with an average thickness of 1.3 nm. Moreover, the GNS was synthesized via a seedless mediation method in the presence of N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) buffer at pH 7.4. The recovery intensity of NS-GQD/GNS was correlated positively to the added amounts of CEA, and an excellent analytical performance with a wide dynamic range of 0.1 pg mL −1 to 10 μg mL −1 and a limit of detection of 64 fg mL −1 was achieved. Additionally, the sensing probe exhibits good selectivity toward a series of interferants including common ions, amino acids, prostate-specific antigen, and human serum. The recovery of the FRET−DET immunosensor in human serum application is within 93−106%. Moreover, the stability and reproducibility of the sensing probe are also examined. The obtained results elaborate the facile fabrication of NS-GQD/GNS for the ultrasensitive and selective detection of CEA, which can open an avenue to develop the prognosis platform for multicancer early detection in the biological fluid samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Composites of Gold Nanostars and Nitrogen- and Sulfur-Codoped Graphene Quantum Dots as Plasmon-Enhanced Immunosensors for Cancer Prognosis

Ng,

Lee,

Tran

et al. 2023

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…The development of proteome research has opened a new methodological era for diagnosis and therapy. Various proteins and their changes in expression levels are identified to serve as biomarkers for the occurrence and progressing of diseases [ 1 – 3 ]. Specially, the detection and quantification of a target protein at the level below 1 ng/mL is of great importance for the early disease screening, noninvasive diagnostic and bioscience research.…”

Section: Introductionmentioning

confidence: 99%

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

et al. 2022

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Sensitive quantification of protein biomarkers is highly desired for clinical diagnosis and treatment. Yet, unlike DNA/RNA which can be greatly amplified by PCR/RT-PCR, the amplification and detection of trace amount of proteins remain a great challenge. Here, we combined allosteric probe (AP) with magnetic bead (MB) for assembling an on-bead DNA synthesis system (named as APMB) to amplify protein signals. The AP is designed and conjugated onto the MB, enabling the protein biomarker to be separated and enriched. Once recognizing the biomarker, the AP alters its conformation to initiate DNA synthesis on beads for primary signal amplification. During the DNA synthesis, biotin-dATPs are incorporated into the newly synthesized DNA strands. Then, the biotin-labeled DNA specifically captures streptavidin (STR)–conjugated horseradish peroxidase (HRP), which is used to catalyze a colorimetric reaction for secondary signal amplification. By using carcinoembryonic antigen (CEA) as a protein model, the APMB can quantify protein biomarkers of as low as 0.01 ng/mL. The response values measured by APMB are linearly related to the protein concentrations in the range 0.05 to 20 ng/mL. Clinical examination demonstrated good practicability of the APMB in quantifying serum protein biomarker. The on-bead DNA synthesis could be exploited to improve protein signal amplification, thus facilitating protein biomarker detection of low abundance for early diagnosis. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05404-4.

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Photoelectrochemical immunosensor for carcinoembryonic antigen detection-an attempt for early cancer screening

Mao

Wen

et al. 2023

Biosensors and Bioelectronics

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Autofluorescence free detection of carcinoembryonic antigen in pleural effusion by persistent luminescence nanoparticle-based aptasensors

Cited by 12 publications

References 33 publications

Composites of Gold Nanostars and Nitrogen- and Sulfur-Codoped Graphene Quantum Dots as Plasmon-Enhanced Immunosensors for Cancer Prognosis

Composites of Gold Nanostars and Nitrogen- and Sulfur-Codoped Graphene Quantum Dots as Plasmon-Enhanced Immunosensors for Cancer Prognosis

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

Photoelectrochemical immunosensor for carcinoembryonic antigen detection-an attempt for early cancer screening

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