Autofluorescence detection of tumors in the human lung—Spectroscopical measurements in situ, in an in vivo model and in vitro

Hüttenberger, Dirk; Gabrecht, Tanja; Wagnières, Georges; Weber, Bernd-Claus; Linder, A.; Foth, Hans‐Jochen; Freitag, Lutz

doi:10.1016/j.pdpdt.2008.05.002

Cited by 19 publications

(7 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We should certainly keep investigating the further potential of AFB in early lung cancer detection, but we should also investigate its potential benefits in everyday clinical practice. There is more and more data confirming that AFB is entering daily practice in respiratory endoscopy suites 46–55 …”

Section: Clinical Application Results and Indicationsmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic value of autofluorescence bronchoscopy in lung cancer

et al. 2013

View full text Add to dashboard Cite

The role of autofluorescence bronchoscopy (AFB) was primarily investigated in regard to the detection of precancerous lesions of bronchial mucosa. Most of the results confirmed higher sensitivity for the detection of precancerous bronchial lesions, when compared to white light bronchoscopy (WLB) alone. However, it is commonly known that the specificity of AFB remains low. Our findings agree in terms of the detection of premalignant bronchial lesions and early lung cancer, but regarding the detection of synchronous lesions or in the evaluation of lung cancer extension, the specificity of AFB is significantly higher. There is still an ongoing debate in the scientific community whether or not autofluorescence should be used as a screening tool for lung cancer. Results of the majority of published series did not support the general use of AFB as a screening tool for lung cancer; however, these results suggest its use in groups of patients with a high risk of lung cancer. Despite this, some authors still do not recommend its use even in high-risk cases. In recent years, the indications for AFB have been widening and this tool may find its place in routine bronchoscopy. With new indications for AFB, such as the evaluation of tumor extension or follow up after surgical resection, bronchoscopists may make use of this tool more often. A sharp learning curve and a clear distinction between healthy and pathologically altered mucosa make this technology acceptable for inexperienced bronchoscopists. We also investigate new hardware and software improvements in AFB. The addition of backscattered light analysis, ultraviolet spectra, fluorescence-reflectance or dual digital systems could improve the diagnostic yield of this technology.

show abstract

Section: Clinical Application Results and Indicationsmentioning

confidence: 99%

“…There is more and more data confirming that AFB is entering daily practice in respiratory endoscopy suites. [46][47][48][49][50][51][52][53][54][55]…”

Section: Clinical Application Results and Indicationsmentioning

confidence: 99%

Diagnostic value of autofluorescence bronchoscopy in lung cancer

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Fluorescence spectroscopy detects tissue autofluorescence emitted by endogenous fluorophores (eg, NADH, FAD, tryptophan, keratins, melanin, collagen, elastin and others). Its effectiveness in cancer detection has already been proved in many tissues , including human bladder . Raman spectroscopy is another optical technique widely used for diagnostic applications .…”

Section: Introductionmentioning

confidence: 99%

Label‐free grading and staging of urothelial carcinoma through multimodal fibre‐probe spectroscopy

Baria

Morselli

Anand

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

Urothelial carcinoma (UC) is the most common bladder tumour. Proper treatment requires tumour resection for diagnosing its grade (aggressiveness) and stage (invasiveness). White-light cystoscopy and histopathological examination are the gold standard procedures for clinical and histopathological diagnostics, respectively. However, cystoscopy is limited in terms of specificity, histology requires long tissue processing, both procedures rely on operator's experience. Multimodal optical spectroscopy can provide a powerful tool for detecting, staging and grading bladder tumours in a fast, reliable and labelfree modality. In this study, we collected fluorescence, Raman and reflectance spectra from 50 biopsies obtained from 32 patients undergoing transurethral resection of bladder tumour using a multimodal fibre-probe. Principal component analysis allowed distinguishing normal from pathological tissues, as well as discriminating tumour stages and grades. Each individual spectroscopic technique provided high specificity and sensitivity in classifying all tissues; however, a multimodal approach resulted in a considerable increase in diagnostic accuracy (≥95%), which is of paramount importance for tumour grading and staging. The presented method offers the potential for being applied in cystoscopy and for providing an automated diagnosis of UC at the clinical level, with an improvement with respect to current state-of-the-art procedures.

show abstract

“…17,18 The autofluorescence bronchoscope technique and biomarkers from endobronchial tissue samples are also being used for early diagnosis of the disease. 18,19 Lung cancer staging has recently been re-evaluated and a new system has been proposed. [20][21][22][23][24][25] A new pathologic classification has been suggested for correct evaluation of the different molecular characteristics of the disease, along with patient survival.…”

Section: Introductionmentioning

confidence: 99%

“One-stop shop” spectral imaging for rapid on-site diagnosis of lung cancer: a future concept in nano-oncology

Darwiche¹,

Krauss²,

Oezkan³

et al. 2013

IJN

View full text Add to dashboard Cite

Background There are currently many techniques and devices available for the diagnosis of lung cancer. However, rapid on-site diagnosis is essential for early-stage lung cancer, and in the current work we investigated a new diagnostic illumination nanotechnology. Methods Tissue samples were obtained from lymph nodes, cancerous tissue, and abnormal intrapulmonary lesions at our interventional pulmonary suites. The following diagnostic techniques were used to obtain the samples: endobronchial ultrasound bronchoscopy; flexible bronchoscopy; and rigid bronchoscopy. Flexible and rigid forceps were used because several of the patients were intubated using a rigid bronchoscope. In total, 30 tissue specimens from 30 patients were prepared. CytoViva® illumination nanotechnology was subsequently applied to each of the biopsy tissue slides. Results A spectral library was created for adenocarcinoma, epidermal growth factor receptor mutation-positive adenocarcinoma, squamous cell carcinoma, usual interstitial pneumonitis, non-specific interstitial pneumonitis, typical carcinoid tumor, sarcoidosis, idiopathic pulmonary fibrosis, small cell neuroendocrine carcinoma, thymoma, epithelioid and sarcomatoid mesothelioma, cryptogenic organizing pneumonia, malt cell lymphoma, and Wegener’s granulomatosis. Conclusion The CytoViva software, once it had created a specific spectral library for each entity, was able to identify the same disease again in subsequent paired sets of slides of the same disease. Further evaluation of this technique could make this illumination nanotechnology an efficient rapid on-site diagnostic tool.

show abstract

Autofluorescence detection of tumors in the human lung—Spectroscopical measurements in situ, in an in vivo model and in vitro

Cited by 19 publications

References 17 publications

Diagnostic value of autofluorescence bronchoscopy in lung cancer

Diagnostic value of autofluorescence bronchoscopy in lung cancer

Label‐free grading and staging of urothelial carcinoma through multimodal fibre‐probe spectroscopy

“One-stop shop” spectral imaging for rapid on-site diagnosis of lung cancer: a future concept in nano-oncology

Contact Info

Product

Resources

About

Autofluorescence detection of tumors in the human lung—Spectroscopical measurements in situ, in an in vivo model and in vitro

Cited by 19 publications

References 17 publications

Diagnostic value of autofluorescence bronchoscopy in lung cancer

Diagnostic value of autofluorescence bronchoscopy in lung cancer

Label‐free grading and staging of urothelial carcinoma through multimodal fibre‐probe spectroscopy

&ldquo;One-stop shop&rdquo; spectral imaging for rapid on-site diagnosis of lung cancer: a future concept in nano-oncology

Contact Info

Product

Resources

About

“One-stop shop” spectral imaging for rapid on-site diagnosis of lung cancer: a future concept in nano-oncology