Autofluorescence characterization of advanced glycation end products of hemoglobin

Vigneshwaran, N.; Bijukumar, Gopalakrishnapillai; Karmakar, Nivedita; Anand, Sneh; Misra, Anoop

doi:10.1016/j.saa.2004.03.027

Cited by 27 publications

(16 citation statements)

References 24 publications

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“…The changes of optical properties of erythrocites and blood resulting from amount of glucose and glycated hemoglobin [10,16,20,21] gives an advantage in use of optical diagnostic techniques for monitoring glycated hemoglobin.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of blood proteins glycation by refractive index and spectral measurements

2008

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Monitoring of blood glucose and glycated proteins level is an urgent requirement for diabetic patients. The amount of glycated hemoglobin and glycated albumin depends on blood glucose concentration and reflects the mean glycemia. The purpose of this study is to investigate the effect of presence of glucose and glycation of proteins on optical properties of water solutions of hemoglobin and albumin with different glucose concentrations. We present the results of feasibility study of the refractive index measurements for water solutions of hemoglobin and albumin with glucose by Abbe refractometer. In addition, absorbance spectrum of water solutions of hemoglobin and albumin with different glucose concentrations has been studied. The experimental results show that the changes of optical properties caused by glycation of proteins can be observed by refractive index and spectral measurements. The refractive index measurements can be potentially applied for evaluation of glycated hemoglobin and glycated albumin amount in blood.

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Section: Introductionmentioning

confidence: 99%

Monitoring of blood proteins glycation by refractive index and spectral measurements

2008

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“…Autofl uorescence of erythrocyte has also been used to assess lipid peroxidation in erythrocytes from thalassemic (Senok et al 1998) and uremic patients (Stoya et al 2002). Spontaneous glycation of glycated proteins and carbohydrates may also generate autofl uorescence in erythrocytes (Vigneshwaran et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Age-dependent increase in green autofluorescence of blood erythrocytes

Khandelwal

Saxena

2007

J. Biosci.

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Green auto-fluorescence (GAF) of different age groups of mouse blood erythrocytes was determined by using a double in vivo biotinylation (DIB) technique that enables delineation of circulating erythrocytes of different age groups. A significant increase in GAF was seen for erythrocytes of old age group (age in circulation more than 40 days) as compared to young erythrocytes (age less than 15 days). Erythrocytes are removed from blood circulation by macrophages in the reticulo-endothelial system and depletion of macrophages results in an increased proportion of aged erythrocytes in the blood. When mice were depleted of macrophages for 7 days by administration of clodronate loaded liposomes, the overall GAF of erythrocytes increased significantly and this increase could be ascribed to an increase in GAF of the oldest population of erythrocytes. Using the DIB technique, the GAF of a cohort of blood erythrocyte generated during a 5 day window was tracked in vivo. GAF of the defined cohort of erythrocytes remained low till 40 days of age in circulation and then increased steeply till the end of the life span of erythrocytes. Taken together our results provide evidence for an age dependent increase in the GAF of blood erythrocytes that is accentuated by depletion of macrophages. Kinetics of changes in GAF of circulating erythrocytes with age has also been defined.

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“…The addition of LB extract was able to prevent large conformational changes arising from enhanced surface hydrophobicity of glycated Hb. Specific binding sites for ANS on the fructated Hb contributed to the observed hyperchromism of the fluorescent intensity [43]. It is likely that LB extract acts to limit access to ANS binding sites, prevents alterations to the protein tertiary structure, and reduces the formation of Hb-AGE adducts.…”

Section: Resultsmentioning

confidence: 97%

“…Auto-fluorescent phenomena associated with the formation of a structurally distinct fluorophore (λ ex /λ em =370/440 nm) is the hallmark of Hb-AGE production at the late stage of Hb glycation [43]. The auto-fluorescent spectra of the samples were recorded at an emission wavelength of 440 nm with and without the LB extract ( Figure 7).…”

Section: Resultsmentioning

confidence: 99%

Protection against Advanced Glycation End Products and the Mode of Action of Lemon Balm on Hemoglobin Fructose-Mediated Glycation

Miroliaei¹,

Shafaei²,

Aminjafari³

et al. 2017

Med chem

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The present communication reports on changes in the secondary and tertiary structures of Hemoglobin (Hb) upon fructation. The presence of Lemon balm (LB) provided significant protection toward the formation of early (HbA1c) and advanced glycation end products (Hb-AGEs), as evident from circular dichroism (CD) and fluorescence studies. The degree of AGE modification and its inhibition were characterized with intrinsic fluorescence, the fibrillar state, and the exposure of hydrophobic clusters. An increased signal of intrinsic fluorescence was observed upon glycation, particularly in the later stage of fructation. Glycation mediated fibril formation and its inhibition were assessed by Thioflavin T (ThT) assay. These sequence of events were parallel with an increase in β-sheet content from ~2 to 13% for Hb-AGE, as evidenced by Congo red binding and CD. Furthermore, the late stage of glycation was also marked by an increase loss of heme moiety, confirming the relatively high affinity of ANS toward glycated-globin which was mitigated by applying the balm extract. However, the activity of balm can be attributed to inhibition of oxidative stress and free radicals derived from Hb-AGE, or chelation of metal ions producing in Fenton reaction. Overall, the presence of LB provided significant protection against AGE-induced deleterious processes, can qualify the herb as an effective AGE-inhibitor with potential prevention toward diabetic complications arising from Hb glycation.

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Autofluorescence characterization of advanced glycation end products of hemoglobin

Cited by 27 publications

References 24 publications

Monitoring of blood proteins glycation by refractive index and spectral measurements

Monitoring of blood proteins glycation by refractive index and spectral measurements

Age-dependent increase in green autofluorescence of blood erythrocytes

Protection against Advanced Glycation End Products and the Mode of Action of Lemon Balm on Hemoglobin Fructose-Mediated Glycation

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