Autocrine TGF-β in Cancer: Review of the Literature and Caveats in Experimental Analysis

Ungefroren, Hendrik

doi:10.3390/ijms22020977

Cited by 37 publications

(21 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromosomal rearrangements can lead to cancer either by forming a hybrid gene or by causing dysregulation of a gene [ 63 ]. Autocrine signaling is defined as the production and secretion of an extracellular mediator by a cell followed by the binding of that mediator to receptors on the same cell to initiate signaling [ 64 ].…”

Section: Drug Resistancementioning

confidence: 99%

Challenges in the Use of Targeted Therapies in Non–Small Cell Lung Cancer

2022

View full text Add to dashboard Cite

Precision oncology has fundamentally changed how we diagnose and treat cancer. In recent years, there has been a significant change in the management of patients with oncogeneaddicted advanced-stage NSCLC. Increasing amounts of identifiable oncogene drivers have led to the development of molecularly targeted drugs. Undoubtedly, the future of thoracic oncology is shifting toward increased molecular testing and the use of targeted therapies. For the most part, these novel drugs have proven to be safe and effective. As with all great innovations, targeted therapies pose unique challenges. Drug toxicities, resistance, access, and costs are some of the expected obstacles that will need to be addressed. This review highlights some of the major challenges in the use of targeted therapies in NSCLC and provides guidance for the future strategies.

show abstract

Section: Drug Resistancementioning

confidence: 99%

Challenges in the Use of Targeted Therapies in Non–Small Cell Lung Cancer

2022

View full text Add to dashboard Cite

show abstract

“…Aberrant activation of the PI3K/Akt pathway is often observed during the EMT process and correlates with the progression of different types of human cancer. For example, in lung cancer, collagen I induces EMT by autocrine activation of TGF-β3 signaling and that PI3K is necessary for TGF-β up-regulation [ 41 , 47 ], similar to the observed activation collagen I and TGF-β3 observed in the KD-SDHB hPheo1 cells ( Figure 3 A).…”

Section: Discussionmentioning

confidence: 64%

Loss of SDHB Induces a Metabolic Switch in the hPheo1 Cell Line toward Enhanced OXPHOS

Tabebi

Dutta

Skoglund

et al. 2022

IJMS

View full text Add to dashboard Cite

Background: Enzymes of tricarboxylic acid (TCA) have recently been recognized as tumor suppressors. Mutations in the SDHB subunit of succinate dehydrogenase (SDH) cause pheochromocytomas and paragangliomas (PCCs/PGLs) and predispose patients to malignant disease with poor prognosis. Methods: Using the human pheochromocytoma cell line (hPheo1), we knocked down SDHB gene expression using CRISPR-cas9 technology. Results: Microarray gene expression analysis showed that >500 differentially expressed gene targets, about 54%, were upregulated in response to SDHB knock down. Notably, genes involved in glycolysis, hypoxia, cell proliferation, and cell differentiation were up regulated, whereas genes involved in oxidative phosphorylation (OXPHOS) were downregulated. In vitro studies show that hPheo1 proliferation is not affected negatively and the cells that survive by shifting their metabolism to the use of glutamine as an alternative energy source and promote OXPHOS activity. Knock down of SDHB expression results in a significant increase in GLUD1 expression in hPheo1 cells cultured as monolayer or as 3D culture. Analysis of TCGA data confirms the enhancement of GLUD1 in SDHB mutated/low expressed PCCs/PGLs. Conclusions: Our data suggest that the downregulation of SDHB in PCCs/PGLs results in increased GLUD1 expression and may represent a potential biomarker and therapeutic target in SDHB mutated tumors and SDHB loss of activity-dependent diseases.

show abstract

“…IL-8 attracts and activates neutrophils [ [48] , [49] , [50] ], and TNF-α promotes T lymphocytes to secrete inflammatory factors, thereby strengthening the inflammatory response of local tissues [ [51] , [52] , [53] ]. Besides, TGF-β inhibits the proliferation of immunocompetent cells and the secretion of some pro-inflammatory cytokines (TNF-α and IFN-γ) [ [54] , [55] , [56] ]. The monocytes in the PPMT group secreted fewer IL-8 and TNF-α and more TGF-β than that of PLCL and PP group ( Fig.…”

Section: Resultsmentioning

confidence: 99%

A functional PVA aerogel-based membrane obtaining sutureability through modified electrospinning technology and achieving promising anti-adhesion effect after cardiac surgery

Jin

Yang

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

Pericardial barrier destruction, inflammatory cell infiltration, and fibrous tissue hyperplasia, trigger adhesions after cardiac surgery. There are few anti-adhesion materials that are both functional and sutureable for pericardial reconstruction. Besides, a few studies have reported on the mechanism of preventing pericardial adhesion. Herein, a functional barrier membrane with sutureability was developed via a modified electrospinning method. It was composed of poly( l -lactide- co -caprolactone) (PLCL) nanofibers, poly(vinyl alcohol) (PVA) aerogel, and melatonin, named PPMT. The PPMT had a special microstructure manifested as a staggered arrangement of nanofibers on the surface and a layered macroporous aerogel structure in a cross-section. Besides providing the porosity and hydrophilicity obtained from PVA, the structure also had suitable mechanical properties for stitching due to the addition of PLCL nanofibers. Furthermore, it inhibited the proliferation of fibroblasts by suppressing the activation of Fas and P53 , and achieved anti-inflammatory effects by affecting the activity of inflammatory cells and reducing the release of pro-inflammatory factors, such as interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α). Finally, in vivo transplantation showed that it up-regulated the expression of matrix metalloproteinase-1 (MMP1) and tissue inhibitor of metalloproteinase-1 (TIMP1), and down-regulated the expression of Vinculin and transforming growth factor β (TGF-β) in the myocardium, thereby reducing the formation of adhesions. Collectively, these results demonstrate a great potential of PPMT membrane for practical application to anti-adhesion.

show abstract

Autocrine TGF-β in Cancer: Review of the Literature and Caveats in Experimental Analysis

Cited by 37 publications

References 113 publications

Challenges in the Use of Targeted Therapies in Non–Small Cell Lung Cancer

Challenges in the Use of Targeted Therapies in Non–Small Cell Lung Cancer

Loss of SDHB Induces a Metabolic Switch in the hPheo1 Cell Line toward Enhanced OXPHOS

A functional PVA aerogel-based membrane obtaining sutureability through modified electrospinning technology and achieving promising anti-adhesion effect after cardiac surgery

Contact Info

Product

Resources

About