Autocrine regulation of ecdysone synthesis by β3-octopamine receptor in the prothoracic gland is essential for
            <i>Drosophila</i>
            metamorphosis

Ohhara, Yuya; Shimada-Niwa, Yuko; Niwa, Ryusuke; Kayashima, Yasunari; Hayashi, Yoshiki; Akagi, Kenzo; Ueda, Hitoshi; Yamakawa‐Kobayashi, Kimiko; Kobayashi, Satoru

doi:10.1073/pnas.1414966112

Cited by 53 publications

(53 citation statements)

References 33 publications

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“…Drosophila metamorphosis involves the coordinated endocrine regulation of transcription throughout the entire organism. A key regulator of metamorphosis and development in insects like Drosophila is the hormone, ecdysone (Ables et al, 2015; Ohhara et al, 2015). Several points during metamorphosis, which mark the transitions between the different larval and pupal stages of development, are controlled by the release of ecdysone pulses.…”

Section: Cap’n’collar Keap1 and Endocrine Signalingmentioning

confidence: 99%

Mechanisms and functions of Nrf2 signaling in Drosophila

Pitoniak

Bohmann

2015

Free Radical Biology and Medicine

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The Nrf2 transcription factor belongs to the Cap’n’collar family, named after the founding member of this group, the product of the Drosophila cap’n’collar gene. The encoded protein, Cap’n’collar, abbreviated Cnc, offers a convenient and accessible model to study the structure, function and biology of Nrf2 transcription factors at organism, tissue, cell and molecular levels, using the powerful genetic, genomic and biochemical tools available in Drosophila. In this review we provide an account of the original identification of Cnc as a regulator of embryonic development. We will then describe the discovery of Nrf2 like-functions of Cnc and its role in acute stress signaling and aging. The establishment of Drosophila as a model organism in which the mechanisms and functions of Nrf2 signaling can be studied has led to several discoveries, the regulation of stem cell activity by an Nrf2-mediated redox mechanism, the interaction of Nrf2 with p62 and Myc in the control of tissue growth and unfolded protein response, and more. Several of these more recent lines of investigation will be highlighted. Model organisms like fly and worm remain powerful experimental platforms that can help to unravel the many remaining puzzles regarding the role of Nrf2 and its relatives in controlling the physiology and maintaining the health of multicellular organisms.

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Section: Cap’n’collar Keap1 and Endocrine Signalingmentioning

confidence: 99%

Mechanisms and functions of Nrf2 signaling in Drosophila

Pitoniak

Bohmann

2015

Free Radical Biology and Medicine

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“…In D. melanogaster , three subtypes of OctβRs have been identified. Among them, β 3 -octopamine receptor (OctβR3) plays a role in metamorphosis by regulating ecdysone synthesis [6], and OctβR2 has a strong effect on oviposition [7,8]. Two octopamine receptor subtypes have been isolated in Bombyx mori [9] and a partial sequence of a putative OctβR in the desert locust Schistocerca gregaria ( SgOctβR ) has also been cloned [10].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Jiang

Gui

et al. 2016

IJMS

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The biogenic amine octopamine plays a critical role in the regulation of many physiological processes in insects. Octopamine transmits its action through a set of specific G-protein coupled receptors (GPCRs), namely octopamine receptors. Here, we report on a β-adrenergic-like octopamine receptor gene (BdOctβR1) from the oriental fruit fly, Bactrocera dorsalis (Hendel), a destructive agricultural pest that occurs in North America and the Asia-Pacific region. As indicated by RT-qPCR, BdOctβR1 was highly expressed in the central nervous system (CNS) and Malpighian tubules (MT) in the adult flies, suggesting it may undertake important roles in neural signaling in the CNS as well as physiological functions in the MT of this fly. Furthermore, its ligand specificities were tested in a heterologous expression system where BdOctβR1 was expressed in HEK-293 cells. Based on cyclic AMP response assays, we found that BdOctβR1 could be activated by octopamine in a concentration-dependent manner, confirming that this receptor was functional, while tyramine and dopamine had much less potency than octopamine. Naphazoline possessed the highest agonistic activity among the tested agonists. In antagonistic assays, mianserin had the strongest activity and was followed by phentolamine and chlorpromazine. Furthermore, when the flies were kept under starvation, there was a corresponding increase in the transcript level of BdOctβR1, while high or low temperature stress could not induce significant expression changes. The above results suggest that BdOctβR1 may be involved in the regulation of feeding processes in Bactrocera dorsalis and may provide new potential insecticide leads targeting octopamine receptors.

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“…The duration of the larval growth period prior to CW is regulated by multiple nutrition-dependent signaling pathways including Insulin/insulin-like (IIS) and Target of Rapamycin (TOR) signaling (Caldwell et al, 2005; Colombani, 2005; Koyama et al, 2014; Layalle et al, 2008; Mirth et al, 2005; Walkiewicz and Stern, 2009). This regulatory network, composed of multiple signaling pathways likely endows the larva with the flexibility to adapt to environmental conditions, notably nutrition, before a commitment to metamorphosis via monoaminergic signaling (Ohhara et al, 2015; Shimada-Niwa and Niwa, 2014). …”

Section: Introductionmentioning

confidence: 99%

Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster

Kaieda

Masuda

Nishida

et al. 2017

Developmental Biology

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Steroid hormones regulate life stage transitions, allowing animals to appropriately follow a developmental timeline. During insect development, the steroid hormone ecdysone is synthesized and released in a regulated manner by the prothoracic gland (PG) and then hydroxylated to the active molting hormone, 20-hydroxyecdysone (20E), in peripheral tissues. We manipulated ecdysteroid titers, through temporally controlled over-expression of the ecdysteroid-inactivating enzyme, CYP18A1, in the PG using the GeneSwitch-GAL4 system in the fruit fly Drosophila melanogaster. We monitored expression of a 20E-inducible glue protein gene, Salivary gland secretion 3 (Sgs3), using a Sgs3:GFP fusion transgene. In wild type larvae, Sgs3-GFP expression is activated at the midpoint of the third larval instar stage in response to the rising endogenous level of 20E. By first knocking down endogenous 20E levels during larval development and then feeding 20E to these larvae at various stages, we found that Sgs3-GFP expression could be triggered at an inappropriate developmental stage after a certain time lag. This stage-precocious activation of Sgs3 required expression of the Broad-complex, similar to normal Sgs3 developmental regulation, and a small level of nutritional input. We suggest that these studies provide evidence for a tissue-autonomic regulatory system for a metamorphic event independent from the primary 20E driven developmental progression.

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Autocrine regulation of ecdysone synthesis by β3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis

Cited by 53 publications

References 33 publications

Mechanisms and functions of Nrf2 signaling in Drosophila

Mechanisms and functions of Nrf2 signaling in Drosophila

Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster

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