Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions

Madueño, Laura; Alvarez, Héctor M.; Morelli, Irma Susana

doi:10.1007/s13762-014-0637-5

Cited by 14 publications

(11 citation statements)

References 28 publications

(34 reference statements)

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“…Sphingobium sp. 22B isolated from soil contaminated with PAHs from Argentina has great capacity of degrading PAHs as unique sources of carbon in mineral medium and in phenanthrene microcosms assays 23 , 24 . Biphenyls and polychlorinated biphenyls (PCBs) are difficult to be completely mineralized by environmental microbes because of the accumulation of dead-end intermediates like benzoate and chlorobenzoates during biphenyl and PCB biodegradation.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

et al. 2020

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Background: Green micro-alga, Chlamydomonas reinhardtii (a Chlorophyte), can be cultured in the laboratory heterotrophically or photo-heterotrophically in Tris-Phosphate-Acetate (TAP) medium, which contains acetate as the carbon source. Chlamydomonas can convert acetate in the TAP medium to glucose via the glyoxylate cycle, a pathway present in many microbes and higher plants. A novel bacterial strain, CC4533, was isolated from a contaminated TAP agar medium culture plate of a Chlamydomonas wild type strain. In this article, we present our research on the isolation, and biochemical and molecular characterizations of CC4533. Methods: We conducted several microbiological tests and spectrophotometric analyses to biochemically characterize CC4533. The 16S rRNA gene of CC4533 was partially sequenced for taxonomic identification. We monitored the growth of CC4533 on Tris-Phosphate (TP) agar medium (lacks a carbon source) containing different sugars, aromatic compounds and saturated hydrocarbons, to see if CC4533 can use these chemicals as the sole source of carbon. Results: CC4533 is a Gram-negative, non-enteric yellow pigmented, aerobic, mesophilic bacillus. It is alpha-hemolytic and oxidase-positive. CC4533 can ferment glucose, sucrose and lactose, is starch hydrolysis-negative, resistant to penicillin, polymyxin B and chloramphenicol. CC4533 is sensitive to neomycin. Preliminary spectrophotometric analyses indicate that CC4533 produces b-carotenes. NCBI-BLAST analyses of the partial 16S rRNA gene sequence of CC4533 show 99.55% DNA sequence identity to that of Sphingobium yanoikuyae strain PR86 and S. yanoikuyae strain NRB095. CC4533 can use cyclo-chloroalkanes, saturated hydrocarbons present in car motor oil, polyhydroxyalkanoate, and mono- and poly-cyclic aromatic compounds, as sole carbon sources for growth. Conclusions: Taxonomically, CC4533 is very closely related to the alpha-proteobacterium S. yanoikuyae, whose genome has been sequenced. Future research is needed to probe the potential of CC4533 for environmental bioremediation. Whole genome sequencing of CC4533 will confirm if it is a novel strain of S. yanoikuyae or a new Sphingobium species.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

et al. 2020

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“…22B is a Gram-negative, rod-shaped, chemoheterotrophic, strictly aerobic bacterium with a yellow colony color. It was isolated from soil contaminated with chronic polycyclic aromatic hydrocarbons (PAHs) from Pico Truncado, Argentina ( 1 ), and selected due to its great capacity of degrading PAHs as unique sources of carbon energy in mineral medium (LMM) and in phenanthrene microcosms assays ( 2 ). Sphingobium sp.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Whole-Genome Sequence of Sphingobium sp. 22B, a Polycyclic Aromatic Hydrocarbon–Degrading Bacterium from Semiarid Patagonia, Argentina

et al. 2016

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“…3,10−12 gene-encoding PAH ring-hydroxylating dioxygenase 13 and bphA gene-encoding biphenyl dioxygenase. 14 Nevertheless, few studies have attempted to explore the actual effects of the ABA strategy on the removal efficiency and active degraders of PCBs or PAHs, 10,15 and even fewer have uncovered the real roles of introduced indigenous degraders in ABA. To the best of our knowledge, only one previous study investigated the effects of ABA in PAH-containing wastewater on the diversity of indigenous PAH degraders and discussed the potential mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Toward a More Comprehensive Understanding of Autochthonous Bioaugmentation (ABA): Cases of ABA for Phenanthrene and Biphenyl by Ralstonia sp. M1 in Industrial Wastewater

et al. 2021

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Although autochthonous bioaugmentation (ABA) has been applied to remove organic pollutants from wastewater, few studies have evaluated the roles of autochthonous microbes in eliminating polychlorinated biphenyls or polyaromatic hydrocarbons to reveal the mechanisms of ABA. Little research has been done to date to confirm the actual performance of introduced degraders in pollutant degradation in ABA and to explore a real-world scenario in which multiple pollutants coexist. Here, ABA by autochthonous strain Ralstonia sp. M1 was used to enhance the biodegradation of phenanthrene and biphenyl. The results showed a marked improvement in phenanthrene and biphenyl degradation in ABA, and the stable-isotope probing results first confirmed the direct involvement of Ralstonia sp. M1 in phenanthrene and biphenyl degradation. Additionally, ABA significantly altered the active degrading communities, and the abundance of the active degraders shared by both phenanthrene and biphenyl metabolisms was significantly improved in ABA, indicating their pivotal roles in improving phenanthrene and biphenyl degradation efficiency. Furthermore, ABA by strain Ralstonia sp. M1 promoted the interactions between the active degraders and other core microbes, consistent with the trends of pollutant removal. Collectively, our findings provide a better view of the mechanisms of ABA and offer new insights into the simultaneous degradation of multiple pollutants during ABA.

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Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions

Cited by 14 publications

References 28 publications

Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

Draft Whole-Genome Sequence of Sphingobium sp. 22B, a Polycyclic Aromatic Hydrocarbon–Degrading Bacterium from Semiarid Patagonia, Argentina

Toward a More Comprehensive Understanding of Autochthonous Bioaugmentation (ABA): Cases of ABA for Phenanthrene and Biphenyl by Ralstonia sp. M1 in Industrial Wastewater

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