Autoantibodies to Nuclear Antigens (ANA): Their Immunobiology and Medicine

Tan, Eng M.

doi:10.1016/s0065-2776(08)60836-6

Cited by 910 publications

(354 citation statements)

References 214 publications

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“…The authors showed that the labelling was mainly confined to the condensed chromatin regions of both HeLa and CHO cell nuclei including the peri-and intranucleolar condensed chromatin clumps. Such anti-DNA antibodies used frequently occur in sera from patients suffering from systemic lupus erythematosus (SLE; for reviews see : Stollar, 1975: Stollar, , 1986Tan, 1982;Tan et al, 1988). However, the application of SLE sera is limited because they usually contain a mixture of different antibodies with different specificities (Arana and Seligmann, 1967;Notman et al, 1975;Gilliam et al, 1980) which might include antibodies directed against other nuclear components such as histones and small nuclear RNPs (for review see Tan et al, 1988).…”

Section: Immunolocaliza Tion Of Dnamentioning

confidence: 99%

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Thiry

Scheer

Goessens

1991

Electron Microscopy Reviews

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Abstract-Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure.Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA to po isomerase I are located together.Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component.

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Section: Immunolocaliza Tion Of Dnamentioning

confidence: 99%

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Thiry

Scheer

Goessens

1991

Electron Microscopy Reviews

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“…Antibodies against EBV antigens are also elevated in SS sera (16)(17)(18). Another defined manifestation of active EBV infection is the presence, in the circulation, of infected B cells that can transform into B cell lymphomas (36)(37)(38).…”

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confidence: 99%

Increased expression of human thioredoxin/adult T cell leukemia–derived factor in Sjögren's syndrome

Shimuta

Terauchi

Tsubota

et al. 1996

Arthritis & Rheumatism

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Objective. To determine the involvement of human thioredoxidadult T cell leukemia-derived factor (TRWADF) in Sjogren's syndrome (SS) and the correlation with Epstein-Barr virus (EBV).Methods. Indirect immunohistochemical techniques and reverse transcriptase polymerase chain reaction were utilized to analyze TRX/ADF expression and the presence of EBV, using 6 normal tissues and 23 surgical specimens. The kinetics of expression of TRW ADF induced by EBV was examined in vitro with peripheral blood B cells from EBV-seronegative donors.Results. Marked expression of TRWADF was found in the infiltrating B cells and the epithelial cells of salivary gland tissues from patients with SS (11 of 12 cases), but not in those from patients with other salivary gland inflammatory conditions (0 of 11 cases) or those of normal individuals (0 of 6 cases). In immunohistologic analyses, a striking topographic correlation between TRWADF and EBV was found. The coexistence of TRWADF messenger RNA and EBV DNA was detected by polymerase chain reaction (r = 0.75, P < 0.01).

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“…Patients with systemic autoimmune diseases frequently produce autoantibodies against a variety of nuclear and nucleolar proteins and RNA-protein complexes [3]. Recently, autoimmune sera were shown to be valuable tools in mapping the topology of nucleolar proteins (for review, see Ref.…”

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confidence: 99%

Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus

Reimer

Raška

Scheer

et al. 1988

Experimental Cell Research

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108

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Certain autoimmune sera contain antibodies against a nucleolar ribonucleoprotein particle associated with 7-2-RNA (R. Reddy et al. (1983) J . Bioi. Chem . 258, 1383C. Hashimoto and J. A. Steitz (1983) J. Bioi. Chem. 258, 1379). In this study, we showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-I-j3-D-ribofuranosylbenzimidazole (DRB)-segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7-2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of Mr 40,000 from e 5 S] methionine-Iabeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation , and/or transport of preribosomes .

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Autoantibodies to Nuclear Antigens (ANA): Their Immunobiology and Medicine

Cited by 910 publications

References 214 publications

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Increased expression of human thioredoxin/adult T cell leukemia–derived factor in Sjögren's syndrome

Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus

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