Autoantibodies to dense-fine-speckled 70 (DFS70) do not necessarily rule out connective tissue diseases

Dolscheid‐Pommerich, Ramona; Stoffel‐Wagner, Birgit; Behning, Charlotte; Brossart, Peter; Schäfer, Valentin Sebastian

doi:10.1016/j.semarthrit.2021.12.006

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…While comparing the association between anti-DFS70 antibodies and clinical indicators in SLE patients, there were some differences between our research and published studies (Table 4; Supplementary Table S5). We found significant associations of anti-DFS70-positive SLE patients with a higher frequency of anti-dsDNA and anti-C1q, more rapid ESR, and lower concentrations of C3, which are related to active SLE; however, our findings were distinct from the listed three studies (8,11,14) in Table 4. Choi et al and Aragoń et al reported negative associations between anti-DFS70 and anti-dsDNA, and Mahler et al did not find any association between these two parameters.…”

Section: B C Acontrasting

confidence: 75%

“…In the past few years, increasing concerns have already been raised regarding the prevalence and clinical significance of anti-DFS70 antibodies in SARD cohorts. A recent study suggested anti-DFS70 antibodies were prevalent in connective tissue disease (CTD) patients, while the monospecific anti-DFS70 antibodies were rare (8). In systemic lupus erythematosus (SLE) patients, the positive rates of anti-DFS70 ranged from 0% to 22.1% by different methodologies among several studies (9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

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Anti-DFS70 antibodies in systemic lupus erythematosus: Prevalence in a large Chinese cohort and an unexpected association with anti-dsDNA antibodies by a long-term follow-up

Dai

Chen

et al. 2022

Front. Immunol.

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ObjectiveMonospecific autoantibodies to dense fine speckles 70 (DFS70) antigen are purported to aid in excluding systemic autoimmune rheumatic diseases (SARD) such as systemic lupus erythematosus (SLE). However, the non-isolated anti-DFS70 still has a certain prevalence in SLE patients, and the clinical significance remains unclear. We aimed to investigate the prevalence, clinical relevance, and value of long-term monitoring of anti-DFS70 antibodies in SLE patients.MethodsAnti-DFS70 antibodies were measured by enzyme-linked immunosorbent assay (ELISA) in 851 SLE patients, 211 healthy individuals, and 194 patients with other SARD (except SLE). Demographic, serological, and clinical associations of anti-DFS70 antibodies were analyzed by a stepwise multivariable logistic regression model. The correlation of anti-DFS70 with anti-dsDNA, anti-C1q, and SLE Disease Activity Index 2000 (SLEDAI-2K) was analyzed. Sixty-one SLE patients with follow-up time ranging from 2 to 57 months were measured anti-DFS70 antibodies using both ELISA and line immunoassay. The dynamic variations of anti-DFS70 antibodies were evaluated with anti-dsDNA, anti-C1q, and SLEDAI-2K during the follow-up.ResultsThe prevalence of anti-DFS70 was significantly higher in SLE (20.7% (176/851)) than in healthy individuals (9.5% (20/211), p = 0.0002) and other SARD (10.8% (21/194), p = 0.002). Multivariable analysis revealed that anti-DFS70-positive SLE patients were associated with younger age (odds ratio (OR) = 0.982; 95% confidence interval (CI) = 0.969, 0.995), higher frequencies of anti-dsDNA (OR 1.598; 95% CI 1.107, 2.306) and anti-PCNA (OR 6.101; 95% CI 2.534, 14.688), and higher levels of serum IgG (OR 1.097; 95% CI 1.067, 1.129) and were more likely to be accompanied by mucosal ulcers (OR 5.921; 95% CI 1.652, 21.215). The O.D. value of anti-DFS70 positively correlated with levels of anti-dsDNA (r = 0.183, p < 0.0001) and anti-C1q (r = 0.181, p < 0.0001), respectively, but not with SLEDAI-2K (p = 0.920). During the follow-up, 49 (42 negative and 7 positive) patients remained stable with anti-DFS70 levels. The other 12 patients experienced significant changes in anti-DFS70, and 83.3% (10/12) of them showed similar trends between anti-DFS70 and anti-dsDNA by evaluation of dynamic variations.ConclusionAnti-DFS70 antibodies seem to be prevalent in Chinese SLE patients. The positive association of anti-DFS70 with anti-dsDNA and consistent dynamic variation between anti-DFS70 and anti-dsDNA during the follow-up suggested a potential relationship between anti-DFS70 and anti-dsDNA in patients with SLE.

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Section: B C Acontrasting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Anti-DFS70 antibodies in systemic lupus erythematosus: Prevalence in a large Chinese cohort and an unexpected association with anti-dsDNA antibodies by a long-term follow-up

Dai

Chen

et al. 2022

Front. Immunol.

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“…This only applies in cases of isolated occurrence; if other specific antibodies are found, they take precedence in the interpretation of the results. 19 The prevalence of anti-DFS70 antibodies in the general population is approximately 1%. 15,20 In rare cases it can be detected in patients with CTD, though here the antibody is usually accompanied by other CTD-associated antibodies.…”

Section: Introductionmentioning

confidence: 99%

Anti‐DFS70 antibodies are associated with atopic dermatitis and can cause misdiagnosis of connective tissue disease

Santler

Wimmer

Schlueter

et al. 2023

J Deutsche Derma Gesell

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Background and Objectives: Antinuclear antibodies (ANA) detected by HEp2 cell immunofluorescence staining are a characteristic finding in patients with connective tissue disease (CTD). However, even detection of highly elevated ANA is not conclusive for CTD and can result in misdiagnosis. Anti-DFS70 antibodies are ANA, which may also be highly elevated in people without CTD. Thus, we wanted to evaluate whether they could cause misdiagnosis of CTD. Since anti-DSF70 antibodies have been associated with atopic dermatitis (AD) in Japan, we wanted to investigate this association and its potential diagnostic relevance in Germany. Patients and Methods:We retrospectively analyzed data of 40 patients referred for first consultation on CTD and prospectively analyzed the prevalence of anti-DFS70 antibodies in 110 AD patients and 89 controls. Results: We could not confirm CTD in 75% of our referred patients, 26% of whom had already received systemic treatments. DFS70-typical fluorescence staining was detected in 35% and definitive anti-DFS70 antibodies in 12.5% of these patients. DFS70-typical fluorescence staining was detected in 22% of AD patients and anti-DFS70 antibodies in 10% (versus 5.6% and 0% in control patients, P < 0.001). Conclusions: Anti-DFS70 antibodies are significantly associated with AD and could be responsible for misdiagnosis of CTD.

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“…Dies trifft jedoch nur bei isoliertem Auftreten des Antikörpers zu. Bei Nachweis anderer spezifischer Antikörper spielen diese eine übergeordnete Rolle in der Interpretation der Ergebnisse 19 . Die Prävalenz von Anti‐DFS70‐Antikörpern in der Allgemeinbevölkerung liegt bei etwa 1% 15,20 .…”

Section: Introductionunclassified

“…Bei Nachweis anderer spezifischer Antikörper spielen diese eine übergeordnete Rolle in der Interpretation der Ergebnisse. 19 Die Prävalenz von Anti-DFS70-Antikörpern in der Allgemeinbevölkerung liegt bei etwa 1%. 15,20 In seltenen Fällen können sie bei Patienten mit Kollagenose nachgewiesen werden, wobei in diesen Fällen zumeist auch Kollagenose-assoziierte Antikörper nachweisbar sind.…”

Section: Introductionunclassified

Anti‐DFS70‐Antikörper sind mit atopischer Dermatitis assoziiert und können zur Fehldiagnose einer Kollagenose führen

Santler

Wimmer

Schlüter

et al. 2023

J Deutsche Derma Gesell

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ZusammenfassungHintergrund: Antinukleäre Antikörper (ANA) sind ein typischer Befund bei Patienten mit einer Kollagenose und können mittels Immunfluoreszenz auf HEp2‐Zellen nachgewiesen werden. Selbst hohe Titer sind jedoch nicht beweisend für das Vorliegen einer Kollagenose und können zu Fehldiagnosen führen. Anti‐DFS70‐Antikörper sind ANA, welche auch bei Menschen ohne Kollagenose in hohen Titern vorliegen können. Daher wollten wir evaluieren, ob sie zu einer Fehldiagnose beitragen können. Da in Japan beschrieben wurde, dass Anti‐DFS70‐Antikörper mit atopischer Dermatitis (AD) assoziiert sind, wollten wir diese Assoziation und ihre potenzielle diagnostische Relevanz auch in Deutschland untersuchen.Patienten und Methodik: Wir analysierten retrospektiv die Daten von 40 Patienten, welche zur Mitbeurteilung bei Kollagenose zu uns überwiesen wurden. Prospektiv erhoben wir die Prävalenz von Anti‐DFS70‐Antikörpern bei 110 AD‐Patienten und 89 Kontrollpatienten.Ergebnisse: Bei 75% der überwiesenen Patienten konnten wir keine Kollagenose nachweisen. 26% dieser Patienten erhielten im Vorfeld bereits systemische Therapien. Das DFS70‐typische Fluoreszenzmuster konnte bei 35%, Anti‐DFS70‐Antikörper bei 12,5% dieser Patienten nachgewiesen werden. Bei den AD‐Patienten trat in 22% der Fälle das DFS70‐typische Fluoreszenzmuster auf und in 10% der Fälle zeigten sich Anti‐DFS70‐Antikörper (im Vergleich zu 5,6% und 0% der Kontrollpatienten, P < 0,001).Schlussfolgerungen: Anti‐DFS70‐Antikörper sind signifikant mit AD assoziiert und könnten für die Fehldiagnose einer Kollagenose mitverantwortlich sein.

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Autoantibodies to dense-fine-speckled 70 (DFS70) do not necessarily rule out connective tissue diseases

Cited by 9 publications

References 26 publications

Anti-DFS70 antibodies in systemic lupus erythematosus: Prevalence in a large Chinese cohort and an unexpected association with anti-dsDNA antibodies by a long-term follow-up

Anti-DFS70 antibodies in systemic lupus erythematosus: Prevalence in a large Chinese cohort and an unexpected association with anti-dsDNA antibodies by a long-term follow-up

Anti‐DFS70 antibodies are associated with atopic dermatitis and can cause misdiagnosis of connective tissue disease

Anti‐DFS70‐Antikörper sind mit atopischer Dermatitis assoziiert und können zur Fehldiagnose einer Kollagenose führen

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