Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome

Mack, G; Rees, Jennifer; Sandblom, Olof; Balczon, Ron; Fritzler, Marvin J.; Rattner, J. B.

doi:10.1002/1529-0131(199803)41:3<551::aid-art22>3.3.co;2-o

Cited by 18 publications

(24 citation statements)

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“…For example, in the endosomal compartment, the two known autoantigens are early endosomal protein EEA1 (180 kDa) [44] and CLIP-170 (170 kDa) [45]. There is also a series of centrosomal autoantigens identified as coiled-coil-rich proteins including pericentrin, a 220 kDa protein [46], ninein, a protein with alternatively spliced products of 245 and 249 kDa [47], Cep250 (250 kDa) and Cep110 (110 kDa) [48]. Centromere autoantigens have been described but the two interesting ones related to this discussion are CENP-E [49] and CENP-F [50]; both are high molecular weight proteins (312 to 400 kDa) and have the same type of overall structure as discussed above.…”

Section: Resultsmentioning

confidence: 99%

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Chen³

et al. 2005

Arthritis Res Ther

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There has been some evidence that Behçet's disease (BD) has a significant autoimmune component but the molecular identity of putative autoantigens has not been well characterized. In the initial analysis of the autoantibody profile in 39 Chinese BD patients, autoantibodies to cellular proteins were uncovered in 23% as determined by immunoblotting. We have now identified one of the major autoantibody specificities using expression cloning. Serum from a BD patient was used as a probe to immunoscreen a λZAP expression cDNA library. Candidate autoantigen cDNAs were characterized by direct nucleotide sequencing and their expressed products were examined for reactivity to the entire panel of BD sera using immunoprecipitation. Reactivity was also examined with normal control sera and disease control sera from patients with lupus and Sjögren's syndrome. Six independent candidate clones were isolated from the cDNA library screen and were identified as overlapping partial human kinectin cDNAs. The finding that kinectin was an autoantigen was verified in 9 out of 39 (23%) BD patient sera by immunoprecipitation of the in vitro translation products. Sera from controls showed no reactivity. The significance of kinectin as a participant in autoimmune pathogenesis in BD and the potential use of autoantibody to kinectin in serodiagnostics are discussed.

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Section: Resultsmentioning

confidence: 99%

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Chen³

et al. 2005

Arthritis Res Ther

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“…The lack of correlation with the frequency of antibody, as shown in Table 2, is consistent with the conclusion that it is unlikely that the immune response is merely directed at cross-reactive coiled-coils in these self-proteins. It is interesting to note that large (approximately 100 kDa or greater) coiled-coil rich proteins were noted in many non-Golgi cytoplasmic organelles, including endosomal protein EEA1 [30] and CLIP-170 [31], and the centrosomal proteins pericentrin [32], ninein [33], and Cep250 and Cep110 [34]. The mitotic organelles are also known to be associated with large coiled-coil rich autoantigens, including the mitotic apparatus proteins NuMA [35,36] and centromere-associated protein CENP-E [37] and CENP-F [38].…”

Section: Discussionmentioning

confidence: 99%

Giantin is the major Golgi autoantigen in human anti-Golgi complex sera

et al. 2003

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“…Recombinant proteins were prepared from full-length cDNAs representing five different centrosome autoantigens using previously published protocols [11,22,23]. Briefly, cDNA inserts were amplified using standard polymerase chain reaction protocols, and subcloned in the Eco RI site of the expression vector pGEX-5X (Pharmacia Biotechnology, Uppsala, Sweden).…”

Section: Methodsmentioning

confidence: 99%

“…A family of proteins located at the centrosome that react with human autoantibodies include pericentrin, ninein, enolase, PCM 1 and a newer autoantigen, Mob1 [10,11]. These components are part of the pericentriolar material (PCM) that surrounds the centrioles and mediates functions such as microtubule organization and recruitment of proteins to the centrosome.…”

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confidence: 99%

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Spectrum of centrosome autoantibodies in childhood varicella and post-varicella acute cerebellar ataxia

et al. 2003

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BackgroundSera from children with post-varicella infections have autoantibodies that react with centrosomes in brain and tissue culture cells. We investigated the sera of children with infections and post-varicella ataxia and related conditions for reactivity to five recombinant centrosome proteins: γγ-enolase, pericentrin, ninein, PCM-1, and Mob1.MethodsSera from 12 patients with acute post-varicella ataxia, 1 with post-Epstein Barr virus (EBV) ataxia, 5 with uncomplicated varicella infections, and other conditions were tested for reactivity to cryopreserved cerebellum tissue and recombinant centrosome proteins. The distribution of pericentrin in the cerebellum was studied by indirect immunofluorescence (IIF) using rabbit antibodies to the recombinant protein. Antibodies to phospholipids (APL) were detected by ELISA.ResultsEleven of 12 children with post-varicella ataxia, 4/5 children with uncomplicated varicella infections, 1/1 with post-EBV ataxia, 2/2 with ADEM, 1/2 with neuroblastoma and ataxia, and 2/2 with cerebellitis had antibodies directed against 1 or more recombinant centrosome antigens. Antibodies to pericentrin were seen in 5/12 children with post-varicella ataxia but not in any of the other sera tested. IIF demonstrated that pericentrin is located in axons and centrosomes of cerebellar cells. APL were detected in 75% of the sera from children with post-varicella ataxia and 50% of children with varicella without ataxia and in none of the controls.ConclusionThis is the first study to show the antigen specificity of anti-centrosome antibodies in children with varicella. Our data suggest that children with post-varicella ataxia have unique autoantibody reactivity to pericentrin.

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Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome

Cited by 18 publications

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Giantin is the major Golgi autoantigen in human anti-Golgi complex sera

Spectrum of centrosome autoantibodies in childhood varicella and post-varicella acute cerebellar ataxia

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