Autoantibodies in Celiac Disease: Importance of Fibroblasts

“…It has also been suggested that gliadin degree of mucosal healing can serologically be monitored only using the gliadin antibody test. antibodies are not markers of coeliac disease and increase According to the experience of the Coeliac Disease Study Group in Tampere, Finland, a small-bowel biopsy should be performed in patients ingesting normal amounts of gluten and with a positive IgA class R1-type reticulin [1][2][3][4][5][6][7][8][9][10] or endomysial antibody test [1,4,[11][12][13]. Such a policy will disclose coeliac disease not only in symptomatic patients but also in clinically silent situations.…”

Section: Gliadin Antibodiesmentioning

confidence: 99%

“…However, it should be remembered that these antibody tests are not 100% specific for the presence of villous atrophy. A good correlation is seen between reticulin and endomysial antibody tests [1,13,14]. IgG class reticulin [15] or IgG class endomysial antibodies [4] must be determined in patients with selective IgA deficiency.…”

Section: Gliadin Antibodiesmentioning

confidence: 99%

“…The antibody positivity is mostly seen in patients [2,3,16]. They also usually disappatients ingesting normal amounts of gluten (n>60); II>coeliac dispear completely within 1 year on a gluten-free diet [13].…”

Section: Gliadin Antibodiesmentioning

confidence: 99%

“…We identified and purified extracellular matrix non-collagenous protein molecules in human fetal lung tissue which bound specifically to serum reticulin and endomysial IgA from patients with coeliac disease [21]. We further showed the auto-antigen to be expressed by human fibroblasts [22], and human umbilical cord Wharton's jelly-derived fibroblasts could be used as antigen in a novel whole-cell enzyme-linked immunosorbent assay (ELISA) [13]. …”

Section: Gliadin Antibodiesmentioning

confidence: 99%

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Antibodies in Relation to Gluten Intake

Mäki¹,

Sulkanen²,

Collin

1998

Dig Dis

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Serum reticulin, endomysial and gliadin antibody tests are used in serological screening and case finding of coeliac disease, as well as the novel tissue transglutaminase antibody test. None of these tests are 100% predictive, however. Reticulin/endomysial antibodies predict forthcoming coeliac disease in individuals with normal small-bowel mucosal morphology. The effect of a gluten-free diet can be monitored with serological tests. A positive test result often indicates an inadequate gluten-free diet. However, slight dietary transgressions and minor mucosal damages cannot always be revealed with the serological tests. Therefore, small-bowel mucosal biopsy remains the most sensitive method to monitor the effect of a gluten-free diet.

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Tissue Transglutaminase Is the Target in Both Rodent and Primate Tissues for Celiac Disease–Specific Autoantibodies

Korponay–Szabó,

Sulkanen,

Halttunen

et al. 2000

J. pediatr. gastroenterol. nutr.

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BackgroundEndomysial antibodies have recently been shown to react with tissue transglutaminase. This study was undertaken to investigate whether the tissue distribution of transglutaminase is also compatible with reticulin, jejunal, and fibroblast autoantibody binding patterns.MethodsSera from patients with and without celiac disease, monoclonal tissue transglutaminase antibodies, and sera from mice parenterally immunized against commercially available tissue transglutaminase, transglutaminase complexed with gliadin, or gliadin were used in indirect immunofluorescence and double‐staining studies using both rodent and primate tissues as substrates. Also, antibody competition, affinity chromatography, and potassium thiocyanate extraction studies were undertaken.ResultsTissue transglutaminase antibody binding patterns were identical with the extracellular binding patterns seen with celiac patient sera. Human umbilical cord–derived fibroblasts exhibited both cytoplasmic and extracellular matrix staining. Double staining with patients' sera and tissue transglutaminase antibodies showed complete overlapping. Tissue transglutaminase effectively absorbed reticulin‐endomysial antibodies from celiac sera, and patients' sera blocked the staining of the monoclonal tissue transglutaminase antibodies. Potassium thiocyanate extraction abolished the staining patterns, but they were elicited again after readdition of tissue transglutaminase.ConclusionsReticulin, endomysial, and jejunal antibodies detect transglutaminase in both rodent and primate tissues, indicating that these tissue autoantibodies are identical.

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Autoantibodies in Celiac Disease: Importance of Fibroblasts

Abstract: Wharton's jelly-derived fibroblast autoantibodies tested in a novel whole-cell enzyme-linked immunosorbent assay correlated well with the human umbilical cord but not with gliadin antibodies.

Cited by 15 publications

References 27 publications

No observed local immunological response at cell level after five years of oats in adult coeliac disease

No observed local immunological response at cell level after five years of oats in adult coeliac disease

Antibodies in Relation to Gluten Intake

Tissue Transglutaminase Is the Target in Both Rodent and Primate Tissues for Celiac Disease–Specific Autoantibodies

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